FastQCFastQC Report
Thu 26 May 2016
SRR1548169_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548169_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2765317
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179540.6492564866885062No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33870.12248143702873848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG2250.043.01
CGTTTTT102000.041.735291
TAATGCG1250.039.6000021
TACGGGA12950.039.4401554
ACGGGAC9650.039.4041445
CGGTCTA2400.039.37499631
TAGTACG1950.039.230771
ACGGGAT14300.039.020985
GTTAGCG1450.038.7931021
CGAACTA356.2514773E-638.57142642
GGCGATA8950.038.4636848
AAGGGAT48000.037.968755
GCGATAA1900.037.8947379
ATAGGGA49500.037.681824
TAGGGAC28600.037.6048975
TTTCGCG900.037.51
CCTAACG301.14033704E-437.49999616
CGCAATA601.5643309E-1037.49999627
TAGGGAT46350.037.4271855
GGGCGAT37950.037.23327
Produced by FastQC (version 0.11.5)