##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548169_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2765317
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14947978839316	31.0	31.0	33.0	30.0	34.0
2	31.539254993188845	31.0	31.0	34.0	30.0	34.0
3	31.634252420246938	31.0	31.0	34.0	30.0	34.0
4	35.45625655214212	37.0	35.0	37.0	33.0	37.0
5	35.31061719144677	37.0	35.0	37.0	33.0	37.0
6	35.35335695690584	37.0	35.0	37.0	33.0	37.0
7	35.77502579270297	37.0	35.0	37.0	35.0	37.0
8	35.76552380793956	37.0	35.0	37.0	35.0	37.0
9	37.490523871223445	39.0	37.0	39.0	35.0	39.0
10	36.80963231340204	39.0	37.0	39.0	32.0	39.0
11	36.49671122695879	39.0	35.0	39.0	32.0	39.0
12	35.961693361014305	37.0	35.0	39.0	31.0	39.0
13	35.755648629072184	37.0	35.0	39.0	30.0	39.0
14	36.69383799398044	39.0	35.0	41.0	31.0	41.0
15	36.90700559827318	39.0	35.0	41.0	31.0	41.0
16	36.9794631139938	38.0	35.0	41.0	32.0	41.0
17	36.84679333327789	38.0	35.0	41.0	31.0	41.0
18	36.77891974048545	38.0	35.0	40.0	31.0	41.0
19	36.70015047099483	38.0	35.0	40.0	31.0	41.0
20	36.59492636829702	38.0	35.0	40.0	31.0	41.0
21	36.45892170771018	38.0	35.0	40.0	30.0	41.0
22	36.38906751016249	38.0	35.0	40.0	30.0	41.0
23	36.32677302457548	38.0	35.0	40.0	30.0	41.0
24	36.16914118706825	38.0	34.0	40.0	30.0	41.0
25	36.03891995022632	38.0	34.0	40.0	30.0	41.0
26	35.994871835670196	38.0	34.0	40.0	30.0	41.0
27	35.99063904789216	38.0	34.0	40.0	30.0	41.0
28	36.01567234425565	38.0	35.0	40.0	30.0	41.0
29	36.04062861509187	38.0	35.0	40.0	30.0	41.0
30	35.94348532193597	38.0	35.0	40.0	30.0	41.0
31	35.804656392015815	38.0	34.0	40.0	29.0	41.0
32	35.658952300947774	38.0	34.0	40.0	29.0	41.0
33	35.52496259922461	38.0	34.0	40.0	28.0	41.0
34	35.365724797554854	38.0	34.0	40.0	27.0	41.0
35	35.189109602985845	38.0	34.0	40.0	26.0	41.0
36	35.043546182951175	38.0	34.0	40.0	25.0	41.0
37	34.9844061277604	38.0	34.0	40.0	25.0	41.0
38	34.90434514379364	38.0	34.0	40.0	25.0	41.0
39	34.86522123865004	37.0	34.0	40.0	25.0	41.0
40	34.737529910675704	37.0	33.0	40.0	24.0	41.0
41	34.67176963798364	37.0	33.0	40.0	24.0	41.0
42	34.622671831113756	37.0	33.0	40.0	24.0	41.0
43	34.52808954633411	37.0	33.0	40.0	24.0	41.0
44	34.39356319727539	37.0	33.0	40.0	23.0	41.0
45	34.311110444119066	37.0	33.0	40.0	23.0	41.0
46	34.200971534185776	36.0	33.0	40.0	23.0	41.0
47	34.15352742560798	36.0	33.0	40.0	23.0	41.0
48	34.053020322805665	36.0	33.0	40.0	23.0	41.0
49	33.94505150765717	36.0	33.0	40.0	23.0	41.0
50	33.81549059294106	36.0	33.0	40.0	23.0	41.0
51	33.45948764644343	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	8.0
12	8.0
13	15.0
14	39.0
15	137.0
16	284.0
17	697.0
18	1585.0
19	2979.0
20	5084.0
21	7936.0
22	11838.0
23	17013.0
24	24293.0
25	33987.0
26	44748.0
27	54003.0
28	60443.0
29	69147.0
30	82041.0
31	98386.0
32	121151.0
33	153740.0
34	235173.0
35	297273.0
36	221396.0
37	272827.0
38	389590.0
39	559413.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.09071509703951	24.26372817293641	29.867100227568848	13.778456502455233
2	30.921662869030932	25.440880738085365	30.3243353293673	13.313121063516405
3	28.855606789384364	25.620426157290467	31.776610059533862	13.74735699379131
4	25.841341155462466	27.840750264797855	31.243687432580064	15.07422114715962
5	22.772904516914334	32.86270615629239	30.345598714360776	14.0187906124325
6	22.07605854952615	40.87368645258392	26.819999298452945	10.230255699436992
7	88.78085224948894	3.333252571043392	6.455498592023988	1.430396587443682
8	89.5838343307476	2.5873706341804574	6.210535717966511	1.618259317105417
9	84.3418674965655	5.587713813642342	7.676552091496201	2.393866598295964
10	40.97273477145658	36.38823324776147	13.093435580803215	9.545596399978736
11	34.57354075500205	22.70792100869448	26.913984906612875	15.804553329690593
12	32.847445699715436	22.117319641834914	28.650422356641208	16.384812301808434
13	24.79654231323208	29.21256405685135	28.625072640858175	17.3658209890584
14	18.561090826115052	32.136062520137834	30.2611237698969	19.041722883850206
15	18.30777447938157	26.129264746139413	39.1392379246213	16.423722849857718
16	22.467514574278464	24.124431303897527	36.480121447197554	16.927932674626454
17	21.930180156560713	24.019452381047092	29.48410616215067	24.566261300241525
18	23.4185086194458	24.894867387717213	32.987393488703106	18.699230504133883
19	25.869077577724365	26.565959707331928	28.832101346789536	18.732861368154175
20	27.652742886258608	26.276083356808638	28.346442740561027	17.724731016371724
21	24.970808048408195	26.55637671919711	30.248358506456945	18.22445672593775
22	24.17415435554043	23.720716286776526	30.458497163254698	21.646632194428342
23	20.930656412989904	27.459383499251622	30.09748972721753	21.512470360540945
24	21.761483403168604	25.506370517376485	34.16874087129975	18.56340520815516
25	20.739683732461774	26.86860132129517	32.034157385934414	20.357557560308635
26	19.583541416770665	31.397123729395222	28.25574789436437	20.763586959469745
27	18.763707741282467	30.39185019294352	31.48803554890814	19.356406516865878
28	17.453044262194894	28.6055088801754	34.51716385499384	19.424283002635864
29	19.488651753126316	26.43913880397799	33.710457065139366	20.36175237775633
30	20.972821560783085	28.27259948859389	32.034048899276286	18.720530051346735
31	25.450355239562043	26.916154639775474	28.651796520977523	18.981693599684952
32	26.05173294779586	26.622879040630785	29.016022394539213	18.30936561703414
33	24.942456868416894	27.43725222099311	28.32373286679249	19.29655804379751
34	20.99560375899038	28.06991024898773	30.004299687883883	20.930186304138008
35	20.07560073582884	28.216005615269424	31.327077510462637	20.3813161384391
36	24.864129501247053	27.911013457046696	28.231410720723883	18.99344632098237
37	20.96736106565721	30.78793498177605	29.053052507180915	19.191651445385826
38	21.282876429718545	30.423492134898094	27.29271183014461	21.00091960523875
39	20.670541569013608	30.085990141455753	28.465778064504	20.777690225026642
40	23.043470242290486	27.897054840367307	27.853443203799056	21.20603171354315
41	19.49917495896492	26.363523603261395	29.320689092787553	24.816612344986126
42	21.232900242540005	26.995241413552222	27.852719959411527	23.919138384496243
43	21.335130836717816	26.75505195245247	28.478796463479593	23.43102074735012
44	19.87945685792985	28.466754444427167	29.49889650987572	22.15489218776726
45	19.54611351971582	31.08988951357114	27.381562403153055	21.982434563559984
46	20.991047319348922	30.848615185890083	27.829250679036072	20.331086815724923
47	20.241693809425826	28.422853510103906	29.36216715841258	21.97328552205769
48	21.108429883445552	26.729051316720653	31.20054590486371	20.96197289497009
49	21.271846952808666	26.437873126299806	30.562825166156358	21.72745475473517
50	20.30092752476479	29.709035166673477	29.114636766779363	20.875400541782373
51	19.702081171887347	29.84109959183703	28.077865937250596	22.37895329902503
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1445.0
1	2650.5
2	3856.0
3	16944.5
4	30033.0
5	21046.5
6	12060.0
7	12125.0
8	12190.0
9	12977.5
10	13765.0
11	14091.0
12	14417.0
13	14401.5
14	14386.0
15	13585.0
16	12784.0
17	12428.5
18	12073.0
19	11980.5
20	11888.0
21	12500.0
22	13112.0
23	14726.0
24	16340.0
25	19617.5
26	27616.5
27	32338.0
28	39778.0
29	47218.0
30	57198.0
31	67178.0
32	72535.5
33	77893.0
34	88368.0
35	98843.0
36	103154.0
37	107465.0
38	118413.0
39	129361.0
40	154088.5
41	178816.0
42	199969.0
43	221122.0
44	223656.5
45	226191.0
46	222816.0
47	219441.0
48	213562.0
49	207683.0
50	197444.5
51	187206.0
52	172784.5
53	158363.0
54	147179.5
55	135996.0
56	127091.5
57	118187.0
58	109795.5
59	101404.0
60	91227.5
61	81051.0
62	71004.0
63	60957.0
64	52465.5
65	43974.0
66	36203.5
67	28433.0
68	23170.5
69	17908.0
70	14676.5
71	11445.0
72	9179.5
73	6914.0
74	5704.5
75	3329.0
76	2163.0
77	1656.0
78	1149.0
79	822.5
80	496.0
81	328.0
82	160.0
83	135.5
84	111.0
85	85.0
86	59.0
87	42.0
88	25.0
89	19.5
90	14.0
91	10.0
92	6.0
93	5.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2765317.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.24348813737716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83409068662161	16.566074440476715
2	6.517101426407514	2.638577308311285
3	2.0161293022543063	1.224404688408106
4	1.0456337354247331	0.846690964764478
5	0.669219363952433	0.6773667127737084
6	0.467836731113547	0.5682388389915838
7	0.3265765244643857	0.46277335992584573
8	0.27217536332070386	0.44078229909351885
9	0.2418356486953552	0.44060373770034006
>10	3.683024487047847	19.066214789274703
>50	1.8951390520556837	27.6730527298732
>100	1.0288939626127211	28.343583731423074
>500	0.0018028584839876306	0.22277246501457346
>1k	3.6057169679752616E-4	0.17361215859671134
>5k	0.0	0.0
>10k+	1.8028584839876308E-4	0.655251775372158
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17954	0.6492564866885062	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3387	0.12248143702873848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.616221937665736E-5	0.0	0.0	0.021118736115967898	0.0
2	3.616221937665736E-5	0.0	0.0	0.06664697031117951	0.0
3	3.616221937665736E-5	0.0	0.0	0.09966307660206768	0.0
4	3.616221937665736E-5	0.0	0.0	0.16565912696446736	0.0
5	3.616221937665736E-5	0.0	0.0	0.2886106728451024	0.0
6	3.616221937665736E-5	0.0	0.0	0.49625413650586897	0.0
7	3.616221937665736E-5	0.0	0.0	0.6175783825145544	0.0
8	3.616221937665736E-5	0.0	0.0	0.9087927351547761	0.0
9	3.616221937665736E-5	0.0	0.0	1.0351073674374403	0.0
10	3.616221937665736E-5	0.0	0.0	1.2289730255157003	0.0
11	3.616221937665736E-5	0.0	0.0	1.442727904251122	0.0
12	3.616221937665736E-5	0.0	0.0	1.6177530460341436	0.0
13	7.232443875331472E-5	0.0	0.0	1.6989372285347395	0.0
14	7.232443875331472E-5	0.0	0.0	1.7400175097466222	0.0
15	7.232443875331472E-5	0.0	0.0	1.7852926084061973	0.0
16	7.232443875331472E-5	0.0	0.0	1.874721776924671	0.0
17	7.232443875331472E-5	0.0	0.0	1.9842209771971893	0.0
18	7.232443875331472E-5	0.0	0.0	2.115236697998819	0.0
19	7.232443875331472E-5	0.0	0.0	2.198807586978274	0.0
20	1.0848665812997207E-4	0.0	0.0	2.2957946593464693	0.0
21	1.0848665812997207E-4	0.0	0.0	2.4130325745655923	0.0
22	1.0848665812997207E-4	0.0	0.0	2.542565644372779	0.0
23	1.0848665812997207E-4	0.0	0.0	2.6800544024428303	0.0
24	1.0848665812997207E-4	0.0	0.0	2.7862990029714494	0.0
25	1.0848665812997207E-4	0.0	0.0	2.8776809313362626	0.0
26	1.0848665812997207E-4	0.0	0.0	2.9670739376353596	0.0
27	1.0848665812997207E-4	0.0	0.0	3.0646034432942044	0.0
28	1.0848665812997207E-4	0.0	0.0	3.1628923555599595	0.0
29	1.0848665812997207E-4	0.0	0.0	3.279551675269056	0.0
30	1.0848665812997207E-4	0.0	0.0	3.4302396434115874	0.0
31	1.0848665812997207E-4	0.0	0.0	3.5612915264325933	0.0
32	1.0848665812997207E-4	0.0	0.0	3.689558918561597	0.0
33	1.0848665812997207E-4	0.0	0.0	3.82010453051133	0.0
34	1.0848665812997207E-4	0.0	0.0	3.9548811221281324	0.0
35	1.0848665812997207E-4	0.0	0.0	4.111535856467812	0.0
36	1.4464887750662944E-4	0.0	0.0	4.248662992344096	0.0
37	1.4464887750662944E-4	0.0	0.0	4.398266093905328	0.0
38	1.4464887750662944E-4	0.0	0.0	4.577811513110432	0.0
39	1.4464887750662944E-4	0.0	0.0	4.837130788260442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	225	0.0	43.0	1
CGTTTTT	10200	0.0	41.73529	1
TAATGCG	125	0.0	39.600002	1
TACGGGA	1295	0.0	39.440155	4
ACGGGAC	965	0.0	39.404144	5
CGGTCTA	240	0.0	39.374996	31
TAGTACG	195	0.0	39.23077	1
ACGGGAT	1430	0.0	39.02098	5
GTTAGCG	145	0.0	38.793102	1
CGAACTA	35	6.2514773E-6	38.571426	42
GGCGATA	895	0.0	38.463684	8
AAGGGAT	4800	0.0	37.96875	5
GCGATAA	190	0.0	37.894737	9
ATAGGGA	4950	0.0	37.68182	4
TAGGGAC	2860	0.0	37.604897	5
TTTCGCG	90	0.0	37.5	1
CCTAACG	30	1.14033704E-4	37.499996	16
CGCAATA	60	1.5643309E-10	37.499996	27
TAGGGAT	4635	0.0	37.427185	5
GGGCGAT	3795	0.0	37.2332	7
>>END_MODULE