FastQCFastQC Report
Thu 26 May 2016
SRR1548168_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548168_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1836932
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92100.5013794740360558No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39070.21269159664048534No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG27380.149052877297581No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC22610.12308566675304257No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC20680.11257901762286247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT302.1662145E-645.00000410
CCGAATC650.045.00000416
TAATGCG700.045.0000041
CGAACGT207.0343906E-445.038
CGGTTGC207.0343906E-445.037
CGCGATT207.0343906E-445.043
TCTACGT207.0343906E-445.043
GTATGCG406.8193913E-945.01
TCGCTCA207.0343906E-445.019
ACGTGCG502.1827873E-1145.01
GTACTAC253.8917202E-545.011
CGAATCT406.8193913E-945.017
CGTCGGA207.0343906E-445.025
TACTACG253.8917202E-545.01
CCGGTAC207.0343906E-445.011
CGACTCA207.0343906E-445.020
CGACTAT253.8917202E-545.021
CGCAAAT207.0343906E-445.031
CGTCATT207.0343906E-445.038
ATCGCGA207.0343906E-445.010