##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548168_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1836932
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17730378696653	31.0	31.0	33.0	30.0	34.0
2	31.564164051799413	31.0	31.0	34.0	30.0	34.0
3	31.663824790465842	31.0	31.0	34.0	30.0	34.0
4	35.47504806928074	37.0	35.0	37.0	33.0	37.0
5	35.30655571354846	37.0	35.0	37.0	33.0	37.0
6	35.36556769657233	37.0	35.0	37.0	33.0	37.0
7	35.749383210701325	37.0	35.0	37.0	35.0	37.0
8	35.74146783876594	37.0	35.0	37.0	35.0	37.0
9	37.48149795419754	39.0	37.0	39.0	35.0	39.0
10	36.8510712427025	39.0	37.0	39.0	32.0	39.0
11	36.541450091783474	39.0	35.0	39.0	32.0	39.0
12	35.708877628567635	37.0	35.0	39.0	31.0	39.0
13	35.33282288075988	37.0	35.0	39.0	30.0	39.0
14	36.269207025627516	38.0	35.0	40.0	30.0	41.0
15	36.57757608882637	38.0	35.0	40.0	31.0	41.0
16	36.75459788386288	38.0	35.0	40.0	32.0	41.0
17	36.61801253394246	38.0	35.0	40.0	31.0	41.0
18	36.57906389566952	38.0	35.0	40.0	31.0	41.0
19	36.50839878667256	38.0	35.0	40.0	31.0	41.0
20	36.360534303937214	38.0	35.0	40.0	31.0	41.0
21	36.20355081189723	38.0	34.0	40.0	30.0	41.0
22	36.095695431295226	38.0	34.0	40.0	30.0	41.0
23	36.03289071125115	37.0	34.0	40.0	30.0	41.0
24	35.895661897119766	37.0	34.0	40.0	30.0	41.0
25	35.7892066772205	37.0	34.0	40.0	30.0	41.0
26	35.72728168489634	37.0	34.0	40.0	30.0	41.0
27	35.69353574329371	37.0	34.0	40.0	30.0	41.0
28	35.73801044350036	37.0	34.0	40.0	30.0	41.0
29	35.81276933495633	37.0	34.0	40.0	30.0	41.0
30	35.791024381958614	37.0	34.0	40.0	30.0	41.0
31	35.638466203430504	37.0	34.0	40.0	30.0	41.0
32	35.46593559260767	37.0	34.0	40.0	29.0	41.0
33	35.34912724042044	37.0	34.0	40.0	29.0	41.0
34	35.242523403152646	37.0	34.0	40.0	28.0	41.0
35	35.09947074796454	37.0	34.0	40.0	27.0	41.0
36	34.894374968697804	37.0	34.0	40.0	26.0	41.0
37	34.78585761476201	37.0	33.0	40.0	25.0	41.0
38	34.7449758619263	36.0	33.0	40.0	26.0	41.0
39	34.73703653700845	36.0	34.0	40.0	26.0	41.0
40	34.5760964477727	36.0	33.0	40.0	24.0	41.0
41	34.56265174758783	36.0	33.0	40.0	25.0	41.0
42	34.510665609832046	36.0	33.0	40.0	24.0	41.0
43	34.392722757293136	36.0	33.0	40.0	24.0	41.0
44	34.27661448545727	36.0	33.0	40.0	24.0	41.0
45	34.17842903275679	35.0	33.0	40.0	24.0	41.0
46	34.07229608934898	35.0	33.0	40.0	23.0	41.0
47	34.04334891003042	35.0	33.0	40.0	23.0	41.0
48	33.9699978006807	35.0	33.0	40.0	23.0	41.0
49	33.88664958746431	35.0	33.0	39.0	24.0	41.0
50	33.72377638366581	35.0	33.0	39.0	24.0	41.0
51	33.35541653147749	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	1.0
13	7.0
14	27.0
15	67.0
16	154.0
17	443.0
18	919.0
19	1810.0
20	3190.0
21	5194.0
22	8116.0
23	11557.0
24	15994.0
25	21838.0
26	29038.0
27	34547.0
28	40253.0
29	46221.0
30	55030.0
31	67708.0
32	84600.0
33	109071.0
34	178444.0
35	244532.0
36	136232.0
37	166338.0
38	234804.0
39	340717.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.217397268924486	23.90611084133762	29.510401038252915	13.366090851484977
2	29.44382263469742	26.71726552752089	30.22757510893163	13.611336728850059
3	28.40132351115882	26.855485124109112	31.6051982327054	13.137993132026663
4	26.498204615086458	27.10617486112714	31.690830145046196	14.704790378740205
5	24.220929245067317	32.627065128159344	29.307508388987724	13.844497237785614
6	21.557847541444104	41.456896608039926	26.299884807929747	10.685371042586226
7	87.54864088599905	4.275661810018009	6.659527952041774	1.5161693519411716
8	88.20370051803769	4.05850624846211	5.873924565525561	1.8638686679746448
9	83.5222534094893	6.035008372656146	7.204458303301374	3.2382799145531793
10	45.727005681211935	32.73496242648067	11.9908630259585	9.54716886634889
11	43.01405822316776	20.621939189910133	22.914892875729752	13.449109711192358
12	38.906448360636105	21.56770092741593	24.537544122482487	14.988306589465477
13	22.822673893208894	36.37554356938635	25.365555175695125	15.436227361709633
14	16.697243011717365	39.81214329109624	27.433514142058606	16.057099555127788
15	14.996853449120598	26.251761088597725	43.77875718861667	14.972628273665004
16	18.145690749575923	22.14039496290554	42.15403727519582	17.55987701232272
17	17.634240135181923	22.825069191456187	28.93612828346395	30.60456238989794
18	21.757582752110586	25.69588857943571	32.13793433834241	20.408594330111292
19	27.71229419488582	26.129437562196095	26.539904580028	19.61836366289008
20	30.487138337183957	25.263373930009386	26.308540544777927	17.940947188028737
21	23.215230612782616	27.685347089603752	29.268203722293478	19.831218575320154
22	24.149179174841528	24.211892438043435	27.32240496654204	24.316523420573
23	19.951418996457136	29.83469175777873	27.150487878702094	23.063401367062035
24	20.651118277649907	25.49713326350676	36.29040160441432	17.561346854429015
25	17.968765310855275	27.090714299712783	34.076002813386665	20.864517576045273
26	18.020373100365177	34.494308989118814	28.023682967034162	19.461634943481847
27	19.094827680066544	34.02118314668153	29.431900582057473	17.452088591194446
28	16.066299677941263	30.59367467059205	36.81137897320097	16.52864667826572
29	16.74661881876956	26.134391474480275	36.512402201061334	20.606587505688832
30	19.430332750477426	29.98989619648414	31.965418425940644	18.61435262709779
31	27.271069370014782	27.32425587882404	26.584108720409898	18.820566030751273
32	27.72056886155829	27.984541616129505	27.59759207199831	16.69729745031389
33	25.66632842152023	28.44824958136719	25.90847129888314	19.97695069822944
34	19.452652575054493	29.38813195044781	28.536984493710165	22.62223098078753
35	20.29144247037996	27.212602317342178	30.30155716161513	22.194398050662738
36	27.689429984343462	26.573275439700545	27.620184089558027	18.117110486397973
37	20.61932613727672	32.13907754886953	29.421720564506472	17.81987574934728
38	20.27217120720854	32.98701312841194	24.636295736586874	22.104519927792644
39	20.88700071641193	30.8231333549636	28.108607177620076	20.181258751004393
40	23.5270548937032	27.26121598404296	26.732780527531773	22.47894859472207
41	17.554106521090603	26.722382755594655	28.2533049671953	27.47020575611944
42	21.940605313642532	26.657274194145458	26.391668281678367	25.01045221053365
43	23.055344454775682	26.47789901858098	26.644807755540217	23.82194877110312
44	19.860506540252988	31.364144127273086	27.8604216160424	20.914927716431528
45	18.043563942486713	35.24256749841584	24.853179105160127	21.860689453937326
46	21.678864541529027	32.72886530366938	25.738840632097432	19.85342952270416
47	21.416524944853702	28.530887370898867	28.200445089965225	21.852142594282206
48	23.459605472603233	25.75087156193044	30.076181371983285	20.713341593483047
49	21.17182345345391	25.07556077198285	31.600081004631637	22.152534769931602
50	19.025745101070697	31.716688478397675	28.952350985229717	20.305215435301903
51	18.926285785211427	32.226124864720084	25.96437973751886	22.88320961254962
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1220.0
1	1868.5
2	2517.0
3	8932.5
4	15348.0
5	11083.5
6	6819.0
7	7036.0
8	7253.0
9	7775.5
10	8298.0
11	8583.0
12	8868.0
13	8522.5
14	8177.0
15	7830.5
16	7484.0
17	7096.0
18	6708.0
19	6920.0
20	7132.0
21	7599.5
22	8067.0
23	9068.0
24	10069.0
25	11830.0
26	16485.5
27	19380.0
28	23078.0
29	26776.0
30	32468.0
31	38160.0
32	42966.0
33	47772.0
34	50256.0
35	52740.0
36	57771.5
37	62803.0
38	73982.5
39	85162.0
40	108687.0
41	132212.0
42	154347.0
43	176482.0
44	178546.0
45	180610.0
46	177555.0
47	174500.0
48	160311.0
49	146122.0
50	137978.0
51	129834.0
52	118161.5
53	106489.0
54	93725.5
55	80962.0
56	73981.5
57	67001.0
58	61578.0
59	56155.0
60	52315.0
61	48475.0
62	40573.0
63	32671.0
64	27293.0
65	21915.0
66	18472.5
67	15030.0
68	12145.0
69	9260.0
70	7895.0
71	6530.0
72	5202.0
73	3874.0
74	2784.5
75	1589.5
76	1484.0
77	978.0
78	472.0
79	522.0
80	572.0
81	343.0
82	114.0
83	105.0
84	96.0
85	56.0
86	16.0
87	12.0
88	8.0
89	6.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1836932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.583989589769775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50600558617977	15.332907489070516
2	7.245950831519893	2.693173496418987
3	2.088351830676269	1.164297260431933
4	0.9630765521770134	0.7159121847923593
5	0.5524680463963544	0.5133530211455147
6	0.38074059014560857	0.4245407498201274
7	0.2837661046367452	0.3691454434149155
8	0.21362434258247487	0.31759940469393
9	0.17173004207420767	0.2872286382742021
>10	2.50442796362154	12.128589282702215
>50	1.5735336669571647	21.471887055796948
>100	1.5074777426652082	42.48857462673798
>500	0.006487580269680252	0.7741925043164436
>1k	0.0020642300858073528	0.8138693906422809
>5k	2.9489001225819323E-4	0.5047294517416611
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9210	0.5013794740360558	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3907	0.21269159664048534	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	2738	0.149052877297581	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	2261	0.12308566675304257	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	2068	0.11257901762286247	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.032554280724599496	0.0
2	0.0	0.0	0.0	0.11981935096127674	0.0
3	0.0	0.0	0.0	0.16249921064035033	0.0
4	0.0	0.0	0.0	0.31699594759087435	0.0
5	0.0	0.0	0.0	0.614938386396448	0.0
6	0.0	0.0	0.0	0.839987544449114	0.0
7	0.0	0.0	0.0	0.9782071410373383	0.0
8	0.0	0.0	0.0	1.329499404441754	0.0
9	0.0	0.0	0.0	1.4328782992511426	0.0
10	0.0	0.0	0.0	1.7812308784429691	0.0
11	0.0	0.0	0.0	2.027511089142113	0.0
12	5.443859652943059E-5	0.0	0.0	2.3732506157005266	0.0
13	5.443859652943059E-5	0.0	0.0	2.4667216859415593	0.0
14	5.443859652943059E-5	0.0	0.0	2.505754159653161	0.0
15	5.443859652943059E-5	0.0	0.0	2.583002528128423	0.0
16	5.443859652943059E-5	0.0	0.0	2.7064692650571716	0.0
17	5.443859652943059E-5	0.0	0.0	2.844525545855807	0.0
18	5.443859652943059E-5	0.0	0.0	3.0179669143985732	0.0
19	5.443859652943059E-5	0.0	0.0	3.136044230270908	0.0
20	5.443859652943059E-5	0.0	0.0	3.2482421777180646	0.0
21	5.443859652943059E-5	0.0	0.0	3.386189581323642	0.0
22	5.443859652943059E-5	0.0	0.0	3.533010476163516	0.0
23	5.443859652943059E-5	0.0	0.0	3.682825493812509	0.0
24	5.443859652943059E-5	0.0	0.0	3.7954045114353714	0.0
25	5.443859652943059E-5	0.0	0.0	3.892631844836935	0.0
26	5.443859652943059E-5	0.0	0.0	3.9884437747287325	0.0
27	5.443859652943059E-5	0.0	0.0	4.085344476551119	0.0
28	1.0887719305886118E-4	0.0	0.0	4.193568406451627	0.0
29	1.0887719305886118E-4	0.0	0.0	4.305221967933489	0.0
30	1.0887719305886118E-4	0.0	0.0	4.435493529428417	0.0
31	1.0887719305886118E-4	0.0	0.0	4.5721344067172875	0.0
32	1.0887719305886118E-4	0.0	0.0	4.699847354175332	0.0
33	1.0887719305886118E-4	0.0	0.0	4.816291512151784	0.0
34	1.0887719305886118E-4	0.0	0.0	4.944222213995945	0.0
35	1.0887719305886118E-4	0.0	0.0	5.092621828135173	0.0
36	1.0887719305886118E-4	0.0	0.0	5.228119494896926	0.0
37	1.0887719305886118E-4	0.0	0.0	5.379839863424449	0.0
38	1.0887719305886118E-4	0.0	0.0	5.515718600361907	0.0
39	1.0887719305886118E-4	0.0	0.0	5.6619951092364875	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	30	2.1662145E-6	45.000004	10
CCGAATC	65	0.0	45.000004	16
TAATGCG	70	0.0	45.000004	1
CGAACGT	20	7.0343906E-4	45.0	38
CGGTTGC	20	7.0343906E-4	45.0	37
CGCGATT	20	7.0343906E-4	45.0	43
TCTACGT	20	7.0343906E-4	45.0	43
GTATGCG	40	6.8193913E-9	45.0	1
TCGCTCA	20	7.0343906E-4	45.0	19
ACGTGCG	50	2.1827873E-11	45.0	1
GTACTAC	25	3.8917202E-5	45.0	11
CGAATCT	40	6.8193913E-9	45.0	17
CGTCGGA	20	7.0343906E-4	45.0	25
TACTACG	25	3.8917202E-5	45.0	1
CCGGTAC	20	7.0343906E-4	45.0	11
CGACTCA	20	7.0343906E-4	45.0	20
CGACTAT	25	3.8917202E-5	45.0	21
CGCAAAT	20	7.0343906E-4	45.0	31
CGTCATT	20	7.0343906E-4	45.0	38
ATCGCGA	20	7.0343906E-4	45.0	10
>>END_MODULE