FastQCFastQC Report
Thu 26 May 2016
SRR1548167_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548167_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2780834
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249210.8961699979214869No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC29330.10547195553564147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140050.042.2367021
CGACGGT2150.040.81395728
GACCGAT13250.040.2452859
TACGGGA13050.039.482764
GCGTTAG2000.039.3750041
GCGATAT2350.039.2553189
GGGCGAT39550.038.343877
CGGGTAT1600.037.968756
TTAGGGA47000.037.9148944
GGACCGA16150.037.7554178
TAGGGAC38650.037.7231565
GGCGATA8600.037.674428
AGGGATT63550.037.5295076
TCACGAC2400.037.49999625
CGGTCTA2400.037.49999631
AACGGGA11450.037.1397364
ACGGGAC9900.037.0454525
CGTTAGG4800.037.0312462
ACGGGAT17500.036.8999985
CGAGGGA22700.036.8722464
Produced by FastQC (version 0.11.5)