FastQCFastQC Report
Thu 26 May 2016
SRR1548166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548166_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1553227
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325372.0948000517632No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23610.15200611372323555No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG207.0340524E-445.00000440
ACGCCGA302.1659962E-645.00000412
GTTTCGA207.0340524E-445.00000438
CGACGGT1600.045.00000428
TCCGATT207.0340524E-445.00000444
CCGGATA207.0340524E-445.00000420
GGTCCGT207.0340524E-445.0000048
ATTCGCA207.0340524E-445.00000444
GTGTCGA302.1659962E-645.00000419
ACGTCGA207.0340524E-445.00000428
TTACGTC207.0340524E-445.00000416
TTACGCG302.1659962E-645.0000041
CCGCGTA207.0340524E-445.00000445
CGCACCG207.0340524E-445.00000430
CGACTTA207.0340524E-445.00000424
CGACTAT207.0340524E-445.00000422
ATTGACG700.045.0000041
TATCGTG302.1659962E-645.0000041
TATCGAG302.1659962E-645.0000041
CCGTTCC207.0340524E-445.00000433