##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548166_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1553227
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15765177916686	31.0	31.0	33.0	30.0	34.0
2	31.519644585112157	31.0	31.0	34.0	30.0	34.0
3	31.439520430690425	31.0	31.0	34.0	30.0	34.0
4	35.337460654495445	37.0	35.0	37.0	33.0	37.0
5	35.24839318399693	37.0	35.0	37.0	33.0	37.0
6	35.31960685720761	37.0	35.0	37.0	33.0	37.0
7	35.795394362832994	37.0	35.0	37.0	35.0	37.0
8	35.80553389813595	37.0	35.0	37.0	35.0	37.0
9	37.56234278698477	39.0	37.0	39.0	35.0	39.0
10	36.815127473318455	39.0	37.0	39.0	32.0	39.0
11	36.51674288433049	39.0	35.0	39.0	32.0	39.0
12	35.81656705684359	37.0	35.0	39.0	31.0	39.0
13	35.517798750601166	37.0	35.0	39.0	30.0	39.0
14	36.515392148089106	38.0	35.0	41.0	31.0	41.0
15	36.80097307090335	38.0	35.0	41.0	31.0	41.0
16	36.94428824634133	38.0	35.0	41.0	32.0	41.0
17	36.84026932315753	38.0	35.0	41.0	32.0	41.0
18	36.701753188683945	38.0	35.0	40.0	31.0	41.0
19	36.51316130868186	37.0	35.0	40.0	31.0	41.0
20	36.28640887648747	37.0	35.0	40.0	31.0	41.0
21	36.132176430103264	37.0	35.0	40.0	31.0	41.0
22	36.02753106918693	37.0	34.0	40.0	30.0	41.0
23	35.99315103330035	37.0	34.0	40.0	31.0	41.0
24	35.793480927127845	36.0	34.0	40.0	30.0	41.0
25	35.64838816219394	36.0	34.0	40.0	30.0	41.0
26	35.54270882491741	36.0	34.0	40.0	30.0	41.0
27	35.429865048701835	36.0	34.0	40.0	30.0	41.0
28	35.42839842469903	36.0	34.0	40.0	29.0	41.0
29	35.4401996617365	36.0	34.0	40.0	30.0	41.0
30	35.31999958795463	36.0	34.0	40.0	29.0	41.0
31	35.11152587483993	36.0	34.0	40.0	29.0	41.0
32	34.80895645002308	35.0	34.0	40.0	27.0	41.0
33	34.52519689652575	35.0	33.0	40.0	25.0	41.0
34	34.22909529643768	35.0	33.0	40.0	23.0	41.0
35	33.93131461145087	35.0	33.0	40.0	22.0	41.0
36	33.66770536438009	35.0	33.0	40.0	21.0	41.0
37	33.53152887504531	35.0	33.0	40.0	20.0	41.0
38	33.462049011509585	35.0	33.0	40.0	20.0	41.0
39	33.43923650567496	35.0	33.0	40.0	20.0	41.0
40	33.30920335533698	35.0	33.0	40.0	18.0	41.0
41	33.26343992217493	35.0	33.0	40.0	18.0	41.0
42	33.18886550388321	35.0	33.0	40.0	18.0	41.0
43	33.0709117212101	35.0	32.0	40.0	18.0	41.0
44	32.94253383439768	35.0	32.0	40.0	18.0	41.0
45	32.87646944071923	35.0	32.0	40.0	18.0	41.0
46	32.74950924752145	35.0	32.0	39.0	18.0	41.0
47	32.72409184233857	35.0	32.0	39.0	18.0	41.0
48	32.61108196033162	35.0	32.0	39.0	18.0	41.0
49	32.518226891497505	35.0	31.0	39.0	17.0	41.0
50	32.3506731469386	35.0	31.0	39.0	15.0	41.0
51	31.996686897665313	35.0	31.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	6.0
13	5.0
14	22.0
15	70.0
16	161.0
17	451.0
18	1115.0
19	1999.0
20	3633.0
21	5885.0
22	9031.0
23	13480.0
24	19478.0
25	28634.0
26	38196.0
27	41284.0
28	40953.0
29	43071.0
30	48702.0
31	57795.0
32	70669.0
33	90187.0
34	143741.0
35	207150.0
36	130336.0
37	132530.0
38	173996.0
39	250587.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.327247723610263	23.638077370532447	28.03434398191636	18.00033092394093
2	35.05585468189775	25.030790734387182	28.093768650686602	11.819585933028463
3	26.283666199467305	23.724993191594017	36.653689383457795	13.337651225480887
4	23.41280443875879	26.81932518556528	36.098587006277896	13.669283369398034
5	21.641653151793008	30.48530575376297	35.26464579871455	12.608395295729471
6	19.534749267170863	39.09312676125254	31.949740765515923	9.422383206060672
7	82.159465422633	3.989951243443489	12.265882578657209	1.584700755266294
8	83.2782329949196	3.1294202328442653	11.87179980775508	1.720546964481045
9	79.00609505242956	5.562676930030189	13.276359476110061	2.1548685414301967
10	44.6823934943186	23.375849119285206	21.000407538627645	10.94134984776855
11	37.89504045448604	23.889489430714246	24.830948728035246	13.384521386764458
12	33.55652457754083	23.239101560815	28.925971541828723	14.278402319815456
13	19.243935368107817	37.85332086037649	29.314710599287807	13.588033172227885
14	14.976175407715678	39.978766786825105	31.060366578742194	13.984691226717022
15	13.705594867974868	28.118620137301242	45.67323385442051	12.50255114030338
16	14.873614738863026	24.12686619534685	44.957047488873165	16.04247157691696
17	15.442816793681798	25.178869540640232	31.786532168189197	27.591781497488775
18	19.150903248527097	27.935581856354545	35.78343667731761	17.130078217800744
19	24.122037538621203	29.86453364511433	29.231142646889346	16.782286169375112
20	26.07809418713427	27.615667252758293	29.65393982978663	16.652298730320812
21	19.34688232949852	31.919803093816938	31.351953062881343	17.3813615138032
22	19.69866606748402	28.241783074849973	29.940762039289815	22.118788818376196
23	16.29356172665039	33.57596796862274	28.905498037311993	21.22497226741487
24	17.39880906010519	28.690204329438	38.74610729790301	15.1648793125538
25	15.306391145659969	30.348107520665042	35.99918105981933	18.346320273855657
26	15.110154536329848	37.6375120957851	30.53603884042706	16.716294527457993
27	15.684507158322642	36.99588019008168	31.925339953529008	15.394272698066668
28	14.516616051613834	32.68569243259356	38.256803416371206	14.540888099421398
29	14.662699013086947	29.234876808090508	37.597917110634825	18.504507068187714
30	16.368888771570415	34.099716268130805	33.5825993238593	15.948795636439488
31	23.578910230120904	31.446723498883294	28.65228327861929	16.322082992376515
32	24.225885849267364	31.022059235385424	29.76551399119382	14.986540924153392
33	23.312175232596395	32.18087246745002	27.439002798689437	17.067949501264142
34	17.581847341051887	32.45945376947478	28.987456437468573	20.971242452004763
35	17.915089037210915	31.732579977041347	31.342810806147458	19.00952017960028
36	24.701540727788018	30.601064750999047	27.728850966407357	16.968543554805578
37	18.110038004747537	36.023517489716575	29.94629889900188	15.920145606534009
38	18.16109300186	36.761915676201866	26.1544513454891	18.92253997644903
39	18.533285862272546	36.275058314077725	28.095506967107838	17.096148856541895
40	21.387537043844848	31.6042664723186	26.948089364915752	20.060107118920804
41	16.12642582185347	30.66647695410909	28.736366287735148	24.470730936302292
42	19.938296205255252	30.95053073375624	26.631458247892937	22.479714813095576
43	19.64973567933084	31.770565409949736	27.47331845248634	21.106380458233083
44	17.357218230174983	35.062099744596246	28.8381543715117	18.74252765371707
45	16.151084162199087	39.88863186127977	24.674693396393444	19.285590580127696
46	20.03242282036045	36.28825664246115	26.07893115430005	17.600389382878355
47	19.030637505013758	33.01455614665467	27.550705724275975	20.404100624055594
48	20.407062200180658	30.152450350141997	30.404699377489575	19.035788072187774
49	18.890606459970112	29.853717454048894	30.82884858427004	20.426827501710953
50	17.468728009492494	35.4355802468023	28.440723731946456	18.654968011758744
51	16.891864486002365	36.76648680456881	25.88050555392097	20.461143155507855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1472.0
1	1778.0
2	2084.0
3	21649.5
4	41215.0
5	27455.5
6	13696.0
7	14861.5
8	16027.0
9	19271.5
10	22516.0
11	24706.0
12	26896.0
13	27232.0
14	27568.0
15	25759.0
16	23950.0
17	21535.0
18	19120.0
19	17258.0
20	15396.0
21	14577.5
22	13759.0
23	13506.5
24	13254.0
25	14328.5
26	16687.5
27	17972.0
28	21866.0
29	25760.0
30	27381.5
31	29003.0
32	34150.0
33	39297.0
34	42287.0
35	45277.0
36	48114.5
37	50952.0
38	60329.0
39	69706.0
40	86827.5
41	103949.0
42	120443.0
43	136937.0
44	141816.5
45	146696.0
46	142860.0
47	139024.0
48	131981.5
49	124939.0
50	113575.0
51	102211.0
52	90069.0
53	77927.0
54	67275.0
55	56623.0
56	48751.5
57	40880.0
58	36025.0
59	31170.0
60	27555.0
61	23940.0
62	19701.5
63	15463.0
64	12132.5
65	8802.0
66	7491.5
67	6181.0
68	4885.5
69	3590.0
70	2806.5
71	2023.0
72	1486.5
73	950.0
74	715.0
75	403.5
76	327.0
77	311.0
78	295.0
79	301.0
80	307.0
81	206.0
82	105.0
83	72.5
84	40.0
85	26.5
86	13.0
87	13.0
88	13.0
89	11.5
90	10.0
91	7.5
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1553227.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.321680181168915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50608846296939	21.72239921440973
2	7.5917261807771474	4.148374294684003
3	2.9805901255619487	2.4430419048526097
4	1.7233516633289752	1.88339451940639
5	1.1233475570518303	1.5345871343033752
6	0.7962220904856018	1.3052475185657608
7	0.5495364182759522	1.0509980787628446
8	0.4372402779643173	0.9556911229493179
9	0.35210749988490153	0.8658151651121625
>10	3.3893228869394165	20.987294902691705
>50	1.022986155678874	19.931605898903225
>100	0.5236875615913105	20.225936807925653
>500	0.0028448396177881232	0.5379272950696706
>1k	7.112099044470308E-4	0.30021589556266387
>5k	0.0	0.0
>10k+	2.3706996814901025E-4	2.107470246800948
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32537	2.0948000517632	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2361	0.15200611372323555	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0545316299549261	0.0
2	0.0	0.0	0.0	0.1711919764464563	0.0
3	0.0	0.0	0.0	0.27993332590793235	0.0
4	0.0	0.0	0.0	0.4102426754106129	0.0
5	0.0	0.0	0.0	0.7226245745148648	0.0
6	0.0	0.0	0.0	1.235427918778131	0.0
7	0.0	0.0	0.0	1.5751078239046836	0.0
8	0.0	0.0	0.0	2.233865365461713	0.0
9	0.0	0.0	0.0	2.543543216799605	0.0
10	0.0	0.0	0.0	2.952755778775414	0.0
11	0.0	0.0	0.0	3.402335910977597	0.0
12	0.0	0.0	0.0	3.727722992196247	0.0
13	0.0	0.0	0.0	3.8903521507158967	0.0
14	0.0	0.0	0.0	3.9661942523533265	0.0
15	0.0	0.0	0.0	4.051822431621392	0.0
16	0.0	0.0	0.0	4.224108903592327	0.0
17	0.0	0.0	0.0	4.429874062194386	0.0
18	0.0	0.0	0.0	4.68720927462631	0.0
19	0.0	0.0	0.0	4.831103245050466	0.0
20	0.0	0.0	0.0	4.973323281143065	0.0
21	0.0	0.0	0.0	5.156941000896842	0.0
22	0.0	0.0	0.0	5.356461096800404	0.0
23	0.0	0.0	0.0	5.596219998750987	0.0
24	0.0	0.0	0.0	5.763227139368554	0.0
25	0.0	0.0	0.0	5.9134305545808825	0.0
26	0.0	0.0	0.0	6.051594519023942	0.0
27	0.0	0.0	0.0	6.187955784956094	0.0
28	0.0	0.0	0.0	6.337708525540697	0.0
29	0.0	0.0	0.0	6.498148693011388	0.0
30	0.0	0.0	0.0	6.700372836681309	0.0
31	6.438208967523743E-5	0.0	0.0	6.878839989261068	0.0
32	6.438208967523743E-5	0.0	0.0	7.047842974658566	0.0
33	6.438208967523743E-5	0.0	0.0	7.213369327213601	0.0
34	6.438208967523743E-5	0.0	0.0	7.387394115605768	0.0
35	6.438208967523743E-5	0.0	0.0	7.58607724434355	0.0
36	6.438208967523743E-5	0.0	0.0	7.775424970078424	0.0
37	6.438208967523743E-5	0.0	0.0	7.971468433139521	0.0
38	6.438208967523743E-5	0.0	0.0	8.160687394695044	0.0
39	6.438208967523743E-5	0.0	0.0	8.349970738340243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	20	7.0340524E-4	45.000004	40
ACGCCGA	30	2.1659962E-6	45.000004	12
GTTTCGA	20	7.0340524E-4	45.000004	38
CGACGGT	160	0.0	45.000004	28
TCCGATT	20	7.0340524E-4	45.000004	44
CCGGATA	20	7.0340524E-4	45.000004	20
GGTCCGT	20	7.0340524E-4	45.000004	8
ATTCGCA	20	7.0340524E-4	45.000004	44
GTGTCGA	30	2.1659962E-6	45.000004	19
ACGTCGA	20	7.0340524E-4	45.000004	28
TTACGTC	20	7.0340524E-4	45.000004	16
TTACGCG	30	2.1659962E-6	45.000004	1
CCGCGTA	20	7.0340524E-4	45.000004	45
CGCACCG	20	7.0340524E-4	45.000004	30
CGACTTA	20	7.0340524E-4	45.000004	24
CGACTAT	20	7.0340524E-4	45.000004	22
ATTGACG	70	0.0	45.000004	1
TATCGTG	30	2.1659962E-6	45.000004	1
TATCGAG	30	2.1659962E-6	45.000004	1
CCGTTCC	20	7.0340524E-4	45.000004	33
>>END_MODULE