FastQCFastQC Report
Thu 26 May 2016
SRR1548165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548165_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences443912
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138603.122240444051974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG207.0286036E-445.018
ATATGCG207.0286036E-445.01
CGCGAAT207.0286036E-445.024
GCGATTC207.0286036E-445.09
TTGCACG207.0286036E-445.01
TATACGG253.8869235E-545.02
ACGCGCA207.0286036E-445.017
TATTGCG302.16248E-644.9999961
CGTTTTT61550.043.903331
GACCGAT2400.041.2499969
TGGGCGA1550.040.645166
ATTAGCG501.0786607E-940.51
TCGTAGG950.040.2631572
ATTGCGG950.040.2631572
AATGCGG900.040.02
TAGGGAC5850.040.05
GGACCGA2950.039.661028
TTTACGG800.039.3752
AATCGAG403.4518234E-739.3751
GGGACCG3550.039.2957737
Produced by FastQC (version 0.11.5)