##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548165_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	443912
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899926111481555	31.0	31.0	33.0	31.0	34.0
2	32.34028365982447	33.0	31.0	34.0	31.0	34.0
3	32.187944907999785	33.0	31.0	34.0	30.0	34.0
4	36.064224440880174	37.0	35.0	37.0	35.0	37.0
5	36.07444944042963	37.0	35.0	37.0	35.0	37.0
6	36.159335634089636	37.0	35.0	37.0	35.0	37.0
7	36.29669393933933	37.0	36.0	37.0	35.0	37.0
8	36.31121032997531	37.0	37.0	37.0	35.0	37.0
9	38.170815386833425	39.0	39.0	39.0	37.0	39.0
10	37.837625475319435	39.0	38.0	39.0	35.0	39.0
11	37.629663086377484	39.0	37.0	39.0	35.0	39.0
12	37.43957135648507	39.0	37.0	39.0	35.0	39.0
13	37.4099866640235	39.0	37.0	39.0	35.0	39.0
14	38.695124258862116	40.0	38.0	41.0	35.0	41.0
15	38.774401232676745	40.0	38.0	41.0	35.0	41.0
16	38.803431761249975	40.0	38.0	41.0	35.0	41.0
17	38.72315458919786	40.0	38.0	41.0	35.0	41.0
18	38.43580709690209	40.0	38.0	41.0	35.0	41.0
19	38.19869929175152	40.0	37.0	41.0	35.0	41.0
20	37.901250698336604	40.0	35.0	41.0	34.0	41.0
21	37.84279767161059	40.0	35.0	41.0	34.0	41.0
22	37.76819955306457	39.0	35.0	41.0	34.0	41.0
23	37.676334498729474	39.0	35.0	41.0	34.0	41.0
24	37.52632729009353	39.0	35.0	41.0	34.0	41.0
25	37.42817495359441	39.0	35.0	41.0	33.0	41.0
26	37.39307340193552	39.0	35.0	41.0	33.0	41.0
27	37.35083079529276	39.0	35.0	41.0	33.0	41.0
28	37.3283015552632	39.0	35.0	41.0	33.0	41.0
29	37.25227072032295	39.0	35.0	41.0	33.0	41.0
30	37.12321360990467	39.0	35.0	41.0	33.0	41.0
31	36.91242858945016	39.0	35.0	41.0	33.0	41.0
32	36.5636680242931	39.0	35.0	41.0	32.0	41.0
33	36.13068806430103	39.0	35.0	41.0	30.0	41.0
34	35.63024653534935	39.0	35.0	41.0	26.0	41.0
35	35.20148588008434	39.0	35.0	41.0	23.0	41.0
36	34.98468390131377	38.0	35.0	41.0	21.0	41.0
37	34.862986357656474	38.0	35.0	41.0	21.0	41.0
38	34.74298284344645	38.0	35.0	41.0	20.0	41.0
39	34.63495017030402	38.0	35.0	41.0	18.0	41.0
40	34.54174250752401	38.0	35.0	40.0	18.0	41.0
41	34.42254545946043	38.0	35.0	40.0	18.0	41.0
42	34.34374831047595	38.0	34.0	40.0	18.0	41.0
43	34.258918434284276	38.0	34.0	40.0	18.0	41.0
44	34.127363080971	38.0	34.0	40.0	16.0	41.0
45	34.05513254879345	38.0	34.0	40.0	17.0	41.0
46	33.98011993368055	38.0	34.0	40.0	17.0	41.0
47	33.93521914253275	38.0	34.0	40.0	17.0	41.0
48	33.810460181297195	37.0	34.0	40.0	17.0	41.0
49	33.719809331579235	37.0	33.0	40.0	15.0	41.0
50	33.630311863612604	37.0	33.0	40.0	15.0	41.0
51	33.29707915082268	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	8.0
15	7.0
16	22.0
17	58.0
18	75.0
19	199.0
20	393.0
21	612.0
22	1090.0
23	1813.0
24	3145.0
25	6063.0
26	10478.0
27	12199.0
28	11000.0
29	9178.0
30	8216.0
31	8410.0
32	10155.0
33	13519.0
34	23761.0
35	38256.0
36	36793.0
37	43491.0
38	73329.0
39	131605.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.98572690082719	21.587837229000343	26.61743769035304	23.808998179819422
2	39.519319144334915	22.445439636684746	26.322784696065888	11.71245652291445
3	25.21828650723567	23.23230730415037	39.59793833011948	11.951467858494476
4	22.938780659229757	25.012615112905262	39.49949539548379	12.549108832381192
5	20.925769071347474	28.76425958298041	38.00978572329651	12.300185622375606
6	19.321847573392926	36.526608877435166	34.797662599794556	9.353880949377354
7	79.73292003820576	2.7453639460073167	16.29331939663717	1.2283966191497413
8	80.63715330966498	2.2218367604390057	16.018715421074447	1.1222945088215683
9	76.01844509722648	5.174674259763196	17.05540737803889	1.7514732649714357
10	33.166032907423094	37.36776658436807	21.61329272468417	7.852907783524662
11	23.74614788516643	23.6028762457424	37.06320171565535	15.587774153435815
12	23.508262898952946	22.587584926742238	38.022851375948385	15.881300798356431
13	20.6525167150246	25.108354809061257	37.90030456486871	16.338823911045434
14	16.94998107733064	28.505424498549264	37.613986555893966	16.930607868226137
15	16.523770477031483	26.791571302420298	41.188118365802225	15.496539854745986
16	20.009371226729623	26.320531997332804	39.16451909387446	14.505577682063112
17	19.711113914469536	26.003577285588136	36.14027104471156	18.145037755230767
18	19.697372452197733	26.53206040836923	37.99198940330516	15.77857773612788
19	21.107336589233903	27.825785290778356	35.2015714826362	15.865306637351548
20	22.363216132927246	27.823307322171964	35.14390239506929	14.669574149831499
21	20.99830597055272	26.945655895763128	36.7439943051776	15.31204382850655
22	20.78542594027645	24.68304528825533	37.24837355151471	17.283155219953507
23	17.985321415055235	27.787489412315953	37.108255690317	17.11893348231181
24	17.8609724449891	27.61786119771486	38.60427291895691	15.91689343833913
25	17.96437131683757	28.918569446196546	36.38806790535061	16.728991331615276
26	17.377768566742958	30.785831426048404	35.138270648236585	16.69812935897205
27	16.668619005568672	29.928679558110616	36.910243471679074	16.49245796464164
28	16.24150732577628	29.131449476472817	38.47226477319829	16.154778424552614
29	17.167591774946388	27.631602659986665	38.31975706896863	16.881048496098323
30	17.942520139126675	28.456766205914686	37.367541314494765	16.233172340463874
31	19.83636396402891	28.496188433743626	35.368045919010974	16.299401683216495
32	19.89020346374957	28.969029897817585	34.88709473949792	16.253671898934925
33	19.326127700985783	29.53423200994792	34.08783722900034	17.05180306006596
34	18.075654634251833	30.367730541188344	34.14325361783416	17.413361206725657
35	18.017084467191697	30.826605273117195	33.42982392906702	17.726486330624088
36	19.965668871307827	31.209564057741172	31.855412784515853	16.96935428643515
37	18.817468327055813	32.37578619185785	31.76057416785309	17.046171313233252
38	18.685685451170503	32.440663915370614	31.01830993530249	17.85534069815639
39	18.776469210113717	31.854511705022613	31.29584241921822	18.073176665645445
40	19.81451278631801	31.137027158535922	31.150318081061112	17.898141974084954
41	18.15337274054317	30.975283389500625	31.496783146209157	19.37456072374705
42	18.948124853574583	31.723855178503847	30.409856007496984	18.91816396042459
43	18.99362936798284	31.428526374596764	31.099407089693454	18.478437167726938
44	18.73141523545207	32.35033069617402	30.818720827551406	18.099533240822506
45	17.957838490511634	32.82182054100813	30.447926616086068	18.77241435239417
46	19.173619996756113	32.29108471949395	30.68783903115933	17.847456252590604
47	18.448025734830324	31.705608318765883	31.399016021193386	18.447349925210403
48	18.623961505884047	31.01425507758294	32.02076087152408	18.34102254500892
49	18.734794283551697	30.448827695579304	32.254591000018024	18.56178702085098
50	17.82019859792031	31.682180251941826	32.11740164717332	18.38021950296455
51	17.477337850745194	32.654670295013425	31.268134224801315	18.59985762944007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	345.0
1	516.0
2	687.0
3	10598.5
4	20510.0
5	13675.5
6	6841.0
7	6864.0
8	6887.0
9	7368.0
10	7849.0
11	8420.0
12	8991.0
13	8699.5
14	8408.0
15	7873.0
16	7338.0
17	6694.5
18	6051.0
19	5455.0
20	4859.0
21	4687.0
22	4515.0
23	4623.0
24	4731.0
25	5039.0
26	5949.5
27	6552.0
28	7457.5
29	8363.0
30	9257.5
31	10152.0
32	11493.0
33	12834.0
34	14529.5
35	16225.0
36	17441.5
37	18658.0
38	19653.0
39	20648.0
40	22304.0
41	23960.0
42	25695.5
43	27431.0
44	28162.0
45	28893.0
46	28685.0
47	28477.0
48	27891.5
49	27306.0
50	26290.0
51	25274.0
52	23209.5
53	21145.0
54	19434.5
55	17724.0
56	16215.5
57	14707.0
58	13481.5
59	12256.0
60	11215.0
61	10174.0
62	8500.5
63	6827.0
64	5793.5
65	4760.0
66	3907.0
67	3054.0
68	2546.0
69	2038.0
70	1680.5
71	1323.0
72	1044.5
73	766.0
74	617.5
75	369.5
76	270.0
77	196.5
78	123.0
79	97.0
80	71.0
81	49.0
82	27.0
83	22.5
84	18.0
85	16.0
86	14.0
87	9.0
88	4.0
89	4.0
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.717172584101945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.44491296065851	29.047837272367406
2	11.010292097441182	9.626776796504197
3	6.240009018106547	8.18386653512749
4	4.148745624551154	7.254857139041623
5	3.102404024912097	6.781416609134733
6	2.37465717081057	6.228797841864056
7	1.7464967647794933	5.344633033840856
8	1.2983261539854238	4.540731883538728
9	0.9629951724198933	3.7889483535303734
>10	2.6463370320213206	14.904833068374629
>50	0.014997821439600864	0.4444938540274486
>100	0.009308992617659962	0.719193768900573
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	5.171662565366644E-4	3.13361384374786
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13860	3.122240444051974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014867811638342734	0.0
2	0.0	0.0	0.0	0.04753194326803511	0.0
3	0.0	0.0	0.0	0.08627836147704951	0.0
4	0.0	0.0	0.0	0.12817855791237903	0.0
5	0.0	0.0	0.0	0.23811025608679198	0.0
6	0.0	0.0	0.0	0.49739588026455694	0.0
7	0.0	0.0	0.0	0.6985618771288004	0.0
8	0.0	0.0	0.0	0.9637045180125791	0.0
9	0.0	0.0	0.0	1.1648705148768224	0.0
10	0.0	0.0	0.0	1.3565751770621204	0.0
11	0.0	0.0	0.0	1.4942150696534449	0.0
12	0.0	0.0	0.0	1.5996413703616934	0.0
13	0.0	0.0	0.0	1.6735298888067904	0.0
14	0.0	0.0	0.0	1.7228639910612915	0.0
15	0.0	0.0	0.0	1.758681900917299	0.0
16	0.0	0.0	0.0	1.799005208239471	0.0
17	0.0	0.0	0.0	1.844509722647732	0.0
18	0.0	0.0	0.0	1.903980969201103	0.0
19	0.0	0.0	0.0	1.9456558957631243	0.0
20	0.0	0.0	0.0	1.9925120294112346	0.0
21	0.0	0.0	0.0	2.0386923534394206	0.0
22	0.0	0.0	0.0	2.0945592820198597	0.0
23	0.0	0.0	0.0	2.159437005532628	0.0
24	0.0	0.0	0.0	2.202688821207807	0.0
25	0.0	0.0	0.0	2.2524734632089243	0.0
26	0.0	0.0	0.0	2.302032835336733	0.0
27	0.0	0.0	0.0	2.3549712555641658	0.0
28	0.0	0.0	0.0	2.4076844059182902	0.0
29	0.0	0.0	0.0	2.468056731964894	0.0
30	0.0	0.0	0.0	2.545549568382923	0.0
31	0.0	0.0	0.0	2.6038944655697525	0.0
32	0.0	0.0	0.0	2.6703490781956782	0.0
33	0.0	0.0	0.0	2.731397213862207	0.0
34	0.0	0.0	0.0	2.796049667501667	0.0
35	0.0	0.0	0.0	2.8744435834129285	0.0
36	0.0	0.0	0.0	2.9415740056587794	0.0
37	0.0	0.0	0.0	3.01501198435726	0.0
38	0.0	0.0	0.0	3.1123285696264125	0.0
39	0.0	0.0	0.0	3.238029158932401	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTCG	20	7.0286036E-4	45.0	18
ATATGCG	20	7.0286036E-4	45.0	1
CGCGAAT	20	7.0286036E-4	45.0	24
GCGATTC	20	7.0286036E-4	45.0	9
TTGCACG	20	7.0286036E-4	45.0	1
TATACGG	25	3.8869235E-5	45.0	2
ACGCGCA	20	7.0286036E-4	45.0	17
TATTGCG	30	2.16248E-6	44.999996	1
CGTTTTT	6155	0.0	43.90333	1
GACCGAT	240	0.0	41.249996	9
TGGGCGA	155	0.0	40.64516	6
ATTAGCG	50	1.0786607E-9	40.5	1
TCGTAGG	95	0.0	40.263157	2
ATTGCGG	95	0.0	40.263157	2
AATGCGG	90	0.0	40.0	2
TAGGGAC	585	0.0	40.0	5
GGACCGA	295	0.0	39.66102	8
TTTACGG	80	0.0	39.375	2
AATCGAG	40	3.4518234E-7	39.375	1
GGGACCG	355	0.0	39.295773	7
>>END_MODULE