FastQCFastQC Report
Thu 26 May 2016
SRR1548164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548164_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1938803
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371571.9164917735324323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT172650.043.6316261
GCGATAC1150.043.0434769
CGTTAGG3700.041.9594572
CGGTCTA1850.041.35135331
TATAGCG1200.041.2500041
CTCACGA1750.041.1428624
GCGTTAG2250.041.01
TCACGAC1700.039.70588325
GGCGATA5700.039.4736828
GGGCGAT30650.038.7601977
CAATCGT356.2505096E-638.5714315
GTCGATG1000.038.251
ATAGCGG3300.038.1818162
TTAGGGA22800.038.0921024
GTTTTTT205150.037.6736532
TCACGCG601.5643309E-1037.5000041
ACGCGTT301.140211E-437.50000410
TTAGACG900.037.51
CACGACG1800.037.526
TAAGGGA24100.037.34444
Produced by FastQC (version 0.11.5)