##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548164_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1938803
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126073149257557	31.0	31.0	33.0	30.0	34.0
2	31.499439602682685	31.0	31.0	34.0	30.0	34.0
3	31.457983095755473	31.0	31.0	34.0	30.0	34.0
4	35.35799562926197	37.0	35.0	37.0	33.0	37.0
5	35.2543718985374	37.0	35.0	37.0	33.0	37.0
6	35.31528164542762	37.0	35.0	37.0	32.0	37.0
7	35.74987866224676	37.0	35.0	37.0	35.0	37.0
8	35.74949130984427	37.0	35.0	37.0	35.0	37.0
9	37.47595604091803	39.0	37.0	39.0	35.0	39.0
10	36.78605046515814	39.0	37.0	39.0	32.0	39.0
11	36.497274349173175	39.0	35.0	39.0	32.0	39.0
12	36.00289869574165	38.0	35.0	39.0	31.0	39.0
13	35.79857984539945	38.0	35.0	39.0	30.0	39.0
14	36.76782014469753	39.0	35.0	41.0	31.0	41.0
15	37.00439807448204	39.0	35.0	41.0	31.0	41.0
16	37.09089319544069	39.0	35.0	41.0	32.0	41.0
17	36.9554823259506	39.0	35.0	41.0	31.0	41.0
18	36.82411312546969	38.0	35.0	40.0	31.0	41.0
19	36.65813287889486	38.0	35.0	40.0	31.0	41.0
20	36.434093613430555	38.0	35.0	40.0	31.0	41.0
21	36.28279871652767	38.0	35.0	40.0	30.0	41.0
22	36.21808301307559	38.0	35.0	40.0	30.0	41.0
23	36.14427458591719	38.0	35.0	40.0	30.0	41.0
24	35.948385163423	37.0	34.0	40.0	30.0	41.0
25	35.798389521782255	37.0	34.0	40.0	30.0	41.0
26	35.71422160993149	37.0	34.0	40.0	30.0	41.0
27	35.64949094879676	37.0	34.0	40.0	29.0	41.0
28	35.63473957900828	37.0	34.0	40.0	29.0	41.0
29	35.61952400527542	37.0	34.0	40.0	29.0	41.0
30	35.49270555079603	37.0	34.0	40.0	29.0	41.0
31	35.30760938579113	37.0	34.0	40.0	27.0	41.0
32	35.058928627611984	37.0	34.0	40.0	26.0	41.0
33	34.79490850798147	37.0	33.0	40.0	25.0	41.0
34	34.470842576579464	37.0	33.0	40.0	23.0	41.0
35	34.178617941069824	37.0	33.0	40.0	23.0	41.0
36	33.9377002201874	36.0	33.0	40.0	21.0	41.0
37	33.83224701013976	36.0	33.0	40.0	21.0	41.0
38	33.72721467833504	36.0	33.0	40.0	20.0	41.0
39	33.66632349960259	36.0	33.0	40.0	20.0	41.0
40	33.5179463823813	36.0	33.0	40.0	18.0	41.0
41	33.43689688947253	36.0	33.0	40.0	18.0	41.0
42	33.358457254295566	36.0	32.0	40.0	18.0	41.0
43	33.24864723233872	35.0	32.0	40.0	18.0	41.0
44	33.08168287340178	35.0	32.0	40.0	18.0	41.0
45	33.001256445342825	35.0	32.0	40.0	18.0	41.0
46	32.908839629400205	35.0	32.0	40.0	18.0	41.0
47	32.867156178322396	35.0	31.0	40.0	18.0	41.0
48	32.73300226995729	35.0	31.0	39.0	18.0	41.0
49	32.63183882013799	35.0	31.0	39.0	18.0	41.0
50	32.48340238796825	35.0	31.0	39.0	17.0	41.0
51	32.15369379973107	35.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	6.0
12	6.0
13	10.0
14	35.0
15	102.0
16	275.0
17	711.0
18	1507.0
19	2809.0
20	4869.0
21	7728.0
22	11418.0
23	16512.0
24	23494.0
25	33973.0
26	45876.0
27	51946.0
28	52926.0
29	54963.0
30	61894.0
31	71547.0
32	85656.0
33	107299.0
34	159991.0
35	209321.0
36	164225.0
37	183710.0
38	245364.0
39	340577.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.666602021969226	23.288028747634492	27.364874100153546	18.680495130242733
2	35.34201257167438	24.167334174745964	27.905723273586847	12.58492997999281
3	26.914957321605137	24.358895669131932	35.486225263732315	13.23992174553062
4	24.375761745778192	26.802362075981932	35.11264424492844	13.70923193331143
5	21.784472171747208	30.854346728367965	33.84397486490376	13.517206234981069
6	20.766318187046338	38.73529182696747	30.51831465084385	9.980075335142354
7	83.24858172800434	3.555389588318153	11.641873877851436	1.554154805826069
8	84.0129193115546	2.8221020908261436	11.542070029807052	1.6229085678122017
9	78.98326957406194	5.386828883594672	13.051764413403527	2.5781371289398667
10	39.65539562296943	30.878588489908466	19.10947115307744	10.356544734044666
11	32.92644997970397	22.703286512348082	29.211683703811065	15.158579804136881
12	29.94971639717908	21.834038837365117	31.61883904656636	16.597405718889437
13	21.528334750874638	29.739689901449502	32.575305484879074	16.15666986279679
14	17.00301680985639	32.603776660135146	33.97880032164176	16.414406208366707
15	16.52643409361343	25.881845654251617	41.6329560042975	15.958764247837454
16	19.666567464564476	24.38896576908536	39.672622747127996	16.271844019222172
17	19.72603714766276	24.61116472380123	32.43196962249388	23.23082850604213
18	21.531790491349557	25.716228002535583	35.292549062488554	17.459432443626298
19	24.46669414066308	27.213440457849508	30.315560683576415	18.004304717910998
20	25.582692001198676	27.25516723462879	30.43707896057516	16.725061803597374
21	22.28411035056166	27.210500499535023	33.20693231854913	17.29845683135419
22	22.21210716096478	24.72226420115917	32.07685360503362	20.98877503284243
23	19.30139369497571	28.31907109696034	31.96822988204578	20.411305326018166
24	19.029009136049407	26.455240682008434	37.11522006103766	17.400530120904495
25	19.276997198787086	27.741034029759597	34.274652968867905	18.70731580258541
26	18.364269087679357	31.64158503984159	30.865074997305037	19.129070875174012
27	17.942359280442624	30.913197472873726	32.91499961574229	18.229443630941358
28	16.66017640781451	29.021927446986624	36.61021774775467	17.707678397444194
29	18.205975542641518	26.988095231954972	35.88693642417512	18.918992801228388
30	18.72572922571298	29.332015681840808	33.81870153904239	18.123553553403827
31	23.162538948000392	27.906548525043544	30.37977556255071	18.55113696440536
32	24.351004202077263	27.944819561347906	30.148034637866765	17.55614159870807
33	23.161507383679517	28.4493576706865	29.414747140374757	18.974387805259223
34	19.22428426199052	29.439865731587993	31.15164356564334	20.184206440778148
35	19.499402466367137	29.540288518224905	31.52197515683646	19.4383338585715
36	23.873957281889908	29.13096379570281	28.57092752590129	18.424151396505987
37	19.71102788679407	31.95471638944235	29.708330346094986	18.625925377668594
38	19.88458858378082	31.95466481122631	28.192394998357234	19.96835160663564
39	19.40573642603194	30.854088837287748	29.001502473433348	20.73867226324696
40	21.42992351466343	29.166707499421037	28.672897659019508	20.730471326896026
41	18.468818131599754	27.773992509811467	29.912167455899336	23.845021902689442
42	20.26528739639871	28.53771115476921	28.42176332510317	22.77523812372892
43	20.436733386527667	28.567884411154722	29.39808737659267	21.597294825724944
44	19.773953310367272	30.90200499999226	29.276156473865576	20.047885215774887
45	18.725522912848806	32.88957155523279	27.50274267163812	20.88216286028029
46	20.39737920768639	31.568034503763403	28.25330887150474	19.781277417045466
47	19.82192105128783	29.80834050700355	29.666500412883618	20.703238028825
48	20.45818992440181	28.451420799328247	31.002582521277304	20.08780675499264
49	20.413935815036393	27.82964540492252	30.91856160734226	20.837857172698826
50	19.06516546549598	30.943731776771543	29.78966919279576	20.201433564936718
51	18.222222680695253	32.06916845084312	28.45791965454974	21.250689213911883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1546.0
1	2200.0
2	2854.0
3	27217.5
4	51581.0
5	34830.5
6	18080.0
7	18519.0
8	18958.0
9	21006.0
10	23054.0
11	24603.0
12	26152.0
13	26046.0
14	25940.0
15	24238.0
16	22536.0
17	20355.0
18	18174.0
19	16908.5
20	15643.0
21	15078.0
22	14513.0
23	14987.5
24	15462.0
25	16521.0
26	20760.0
27	23940.0
28	27551.5
29	31163.0
30	34796.5
31	38430.0
32	44049.0
33	49668.0
34	54689.5
35	59711.0
36	65262.5
37	70814.0
38	77736.5
39	84659.0
40	98979.0
41	113299.0
42	125196.5
43	137094.0
44	141715.5
45	146337.0
46	145205.0
47	144073.0
48	140490.0
49	136907.0
50	130275.0
51	123643.0
52	113719.0
53	103795.0
54	93677.5
55	83560.0
56	78023.5
57	72487.0
58	66793.5
59	61100.0
60	56180.0
61	51260.0
62	45896.5
63	40533.0
64	35541.0
65	30549.0
66	25759.5
67	20970.0
68	18566.0
69	16162.0
70	13656.5
71	11151.0
72	9251.0
73	7351.0
74	5687.5
75	3108.0
76	2192.0
77	1533.5
78	875.0
79	685.0
80	495.0
81	386.5
82	278.0
83	201.5
84	125.0
85	78.0
86	31.0
87	29.5
88	28.0
89	22.0
90	16.0
91	11.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1938803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.103617335908304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.87950953565162	20.85144149898688
2	6.439612381987005	3.361943548219315
3	2.5535760155036598	1.9997271344058296
4	1.4837331535007712	1.5492320987033852
5	0.9782215790812254	1.2767560885032136
6	0.6646593885560863	1.041000860255212
7	0.5193025845479483	0.9488973164011469
8	0.43057114881811015	0.89915716037043
9	0.3588286276057689	0.8430052665771121
>10	5.15109488129495	32.592819386672474
>50	1.2556166561843451	22.550574818978316
>100	0.28307963336999203	9.614709870233439
>500	0.0011969530355520365	0.22329619931839806
>1k	7.979686903680243E-4	0.3125001147643906
>5k	0.0	0.0
>10k+	1.9949217259200607E-4	1.9349386376104751
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37157	1.9164917735324323	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0315643208722082E-4	0.0	0.0	0.028161705959811285	0.0
2	1.0315643208722082E-4	0.0	0.0	0.08474300895965191	0.0
3	1.0315643208722082E-4	0.0	0.0	0.15978931330310506	0.0
4	1.0315643208722082E-4	0.0	0.0	0.24009659568300648	0.0
5	1.0315643208722082E-4	0.0	0.0	0.43464962659950496	0.0
6	1.0315643208722082E-4	0.0	0.0	0.8265924903149005	0.0
7	1.0315643208722082E-4	0.0	0.0	1.1098084746103654	0.0
8	1.0315643208722082E-4	0.0	0.0	1.5583326413256013	0.0
9	1.0315643208722082E-4	0.0	0.0	1.810859587075118	0.0
10	1.0315643208722082E-4	0.0	0.0	2.094952401043324	0.0
11	1.0315643208722082E-4	0.0	0.0	2.3375763293124674	0.0
12	1.0315643208722082E-4	0.0	0.0	2.524341049606381	0.0
13	1.0315643208722082E-4	0.0	0.0	2.6209470482560633	0.0
14	1.0315643208722082E-4	0.0	0.0	2.678817806656994	0.0
15	1.0315643208722082E-4	0.0	0.0	2.7271981733059008	0.0
16	1.0315643208722082E-4	0.0	0.0	2.814365358419602	0.0
17	1.0315643208722082E-4	0.0	0.0	2.910249262044674	0.0
18	1.0315643208722082E-4	0.0	0.0	3.0318706954755075	0.0
19	1.0315643208722082E-4	0.0	0.0	3.1006760356776835	0.0
20	1.0315643208722082E-4	0.0	0.0	3.1785591419035355	0.0
21	1.0315643208722082E-4	0.0	0.0	3.276454595954308	0.0
22	1.0315643208722082E-4	0.0	0.0	3.3860067268309364	0.0
23	1.0315643208722082E-4	0.0	0.0	3.507989207774075	0.0
24	1.0315643208722082E-4	0.0	0.0	3.603357329238711	0.0
25	1.0315643208722082E-4	0.0	0.0	3.6875845560379266	0.0
26	1.0315643208722082E-4	0.0	0.0	3.7698002324114417	0.0
27	1.0315643208722082E-4	0.0	0.0	3.8619705044813735	0.0
28	1.0315643208722082E-4	0.0	0.0	3.9539344636871308	0.0
29	1.0315643208722082E-4	0.0	0.0	4.059463493712357	0.0
30	1.0315643208722082E-4	0.0	0.0	4.187686938796773	0.0
31	1.0315643208722082E-4	0.0	0.0	4.300385340852062	0.0
32	1.0315643208722082E-4	0.0	0.0	4.413754259715917	0.0
33	1.0315643208722082E-4	0.0	0.0	4.527381069659992	0.0
34	1.0315643208722082E-4	0.0	0.0	4.647970938769952	0.0
35	1.0315643208722082E-4	0.0	0.0	4.789192094297357	0.0
36	1.0315643208722082E-4	0.0	0.0	4.907821991197662	0.0
37	1.0315643208722082E-4	0.0	0.0	5.03537491947351	0.0
38	1.0315643208722082E-4	0.0	0.0	5.185364371728329	0.0
39	1.0315643208722082E-4	0.0	0.0	5.406789653203549	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	17265	0.0	43.631626	1
GCGATAC	115	0.0	43.043476	9
CGTTAGG	370	0.0	41.959457	2
CGGTCTA	185	0.0	41.351353	31
TATAGCG	120	0.0	41.250004	1
CTCACGA	175	0.0	41.14286	24
GCGTTAG	225	0.0	41.0	1
TCACGAC	170	0.0	39.705883	25
GGCGATA	570	0.0	39.473682	8
GGGCGAT	3065	0.0	38.760197	7
CAATCGT	35	6.2505096E-6	38.57143	15
GTCGATG	100	0.0	38.25	1
ATAGCGG	330	0.0	38.181816	2
TTAGGGA	2280	0.0	38.092102	4
GTTTTTT	20515	0.0	37.673653	2
TCACGCG	60	1.5643309E-10	37.500004	1
ACGCGTT	30	1.140211E-4	37.500004	10
TTAGACG	90	0.0	37.5	1
CACGACG	180	0.0	37.5	26
TAAGGGA	2410	0.0	37.3444	4
>>END_MODULE