FastQCFastQC Report
Thu 26 May 2016
SRR1548163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548163_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1602599
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400332.4980048034474No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTT19830.12373650551385593No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCT17030.10626488597584298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG302.1660398E-645.0000041
CGCCGGT600.045.00000428
CGACGGT551.8189894E-1245.028
TAACGCC453.8562575E-1045.012
CGTTTTT219950.044.0691071
GCGATTC501.0822987E-940.5000049
TACGGGA8250.039.818184
ACGGGAT8200.039.7865875
GTACGCG403.459354E-739.3750041
TAGGGCG4200.039.1071435
GTTTTTT252150.038.6555632
AGTCGGC356.249833E-638.57142630
CGTTCGA659.094947E-1238.0769214
TAGGGAT28600.037.9982535
AGGGCGA11400.037.8947376
ACTCGCC301.1401226E-437.50000442
ATAACGC601.5643309E-1037.50000411
GCGCGAC1800.037.59
CGAGGGA11600.037.435354
GGCACCG5550.037.29738