FastQCFastQC Report
Thu 26 May 2016
SRR1548162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548162_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2235622
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT525192.349189621501309No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTT29200.1306124201676312No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT25820.1154935852304191No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22940.10261126433717328No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.034718E-445.00000421
CGTTCCG207.034718E-445.00000440
CGTACTC207.034718E-445.00000442
CCGGTCA207.034718E-445.00000431
CGTTTTT239000.043.9079551
ACGCGAG800.042.1875041
CGGGACG1500.040.56
ATACCGG1000.040.52
ACGGGAC8000.039.93755
AGGGCGA20550.039.7445266
TATAGCG1250.039.6000021
GGGCGAT39500.039.5886087
ATAGCGG3850.039.1558462
GGCGATC5550.038.918928
ACGTTCG356.250939E-638.5714313
CGTTCGA356.250939E-638.5714314
AGTTCGT356.250939E-638.5714328
TAACGGG7350.038.2653053
GTTTTTT277400.038.2191772
GGACCGA10050.038.05978