##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548162_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2235622
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139374634889084	31.0	31.0	33.0	30.0	34.0
2	31.515161328704046	31.0	31.0	34.0	30.0	34.0
3	31.39657911757891	31.0	31.0	34.0	30.0	34.0
4	35.312167262623106	37.0	35.0	37.0	33.0	37.0
5	35.21781723386154	37.0	35.0	37.0	33.0	37.0
6	35.28216576863173	37.0	35.0	37.0	32.0	37.0
7	35.79493894763963	37.0	35.0	37.0	35.0	37.0
8	35.81912103208861	37.0	35.0	37.0	35.0	37.0
9	37.545403918909365	39.0	37.0	39.0	35.0	39.0
10	36.83766799575241	39.0	37.0	39.0	32.0	39.0
11	36.514366471612824	39.0	35.0	39.0	32.0	39.0
12	36.122438855942555	38.0	35.0	39.0	31.0	39.0
13	35.958450489394004	38.0	35.0	39.0	31.0	39.0
14	36.96045395867459	39.0	35.0	41.0	31.0	41.0
15	37.163868489395796	39.0	35.0	41.0	32.0	41.0
16	37.241122604805284	39.0	35.0	41.0	32.0	41.0
17	37.086856364805854	39.0	35.0	41.0	32.0	41.0
18	36.91133161151572	39.0	35.0	40.0	31.0	41.0
19	36.72820494699015	38.0	35.0	40.0	31.0	41.0
20	36.49974906312427	38.0	35.0	40.0	31.0	41.0
21	36.36215961374508	38.0	35.0	40.0	31.0	41.0
22	36.2847180784587	38.0	35.0	40.0	31.0	41.0
23	36.209415992506784	38.0	35.0	40.0	30.0	41.0
24	36.02142177881592	37.0	35.0	40.0	30.0	41.0
25	35.91904534845336	37.0	34.0	40.0	30.0	41.0
26	35.835490972982015	37.0	34.0	40.0	30.0	41.0
27	35.74825126966902	37.0	34.0	40.0	30.0	41.0
28	35.73057073154585	37.0	34.0	40.0	30.0	41.0
29	35.709467879632605	37.0	34.0	40.0	30.0	41.0
30	35.57457700809886	37.0	34.0	40.0	29.0	41.0
31	35.359872554483715	37.0	34.0	40.0	28.0	41.0
32	35.053802029144464	37.0	34.0	40.0	26.0	41.0
33	34.72850240335799	37.0	33.0	40.0	24.0	41.0
34	34.36646087755444	37.0	33.0	40.0	23.0	41.0
35	34.0287105780852	37.0	33.0	40.0	21.0	41.0
36	33.78854475398793	37.0	33.0	40.0	19.0	41.0
37	33.67315852143162	37.0	33.0	40.0	18.0	41.0
38	33.555580505112225	36.0	33.0	40.0	18.0	41.0
39	33.50885391179725	36.0	33.0	40.0	18.0	41.0
40	33.38279905994842	36.0	33.0	40.0	18.0	41.0
41	33.298083933688254	36.0	32.0	40.0	18.0	41.0
42	33.23824734235036	36.0	32.0	40.0	17.0	41.0
43	33.123304834180374	36.0	32.0	40.0	17.0	41.0
44	32.96901533443489	36.0	32.0	40.0	15.0	41.0
45	32.879744876369976	35.0	31.0	40.0	15.0	41.0
46	32.77011453635722	35.0	31.0	40.0	15.0	41.0
47	32.72989262048772	35.0	31.0	40.0	15.0	41.0
48	32.59696272446773	35.0	31.0	40.0	15.0	41.0
49	32.48199874576292	35.0	31.0	40.0	15.0	41.0
50	32.340596039938774	35.0	31.0	39.0	13.0	41.0
51	32.03953172763553	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	11.0
14	33.0
15	90.0
16	278.0
17	734.0
18	1710.0
19	3366.0
20	5737.0
21	8848.0
22	13239.0
23	19660.0
24	28307.0
25	42030.0
26	58005.0
27	63864.0
28	62747.0
29	63487.0
30	69436.0
31	81112.0
32	95697.0
33	119013.0
34	173226.0
35	233537.0
36	183041.0
37	210684.0
38	284866.0
39	412781.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.523883733475518	23.00165233657568	28.422112503813256	21.052351426135544
2	35.95017404552291	23.923498695217706	27.675161543409395	12.451165715849996
3	25.706313500224994	23.74972155400153	38.12433407794341	12.41963086783007
4	22.4031611784103	26.633840604538694	37.34875573777678	13.614242479274225
5	20.84654740380977	30.20846100101001	36.15647010093836	12.78852149424187
6	20.083717193693744	37.66808521297428	32.7900691619603	9.458128431371673
7	82.11790723118668	2.906663112100346	13.540124403857181	1.4353052528558048
8	83.22328193227656	2.1011154837445685	13.195477589681975	1.480124994296889
9	78.59562126334416	4.936523258404149	14.502720048380272	1.9651354298714185
10	36.08807750147386	36.08642248108133	19.4431795715018	8.382320445943007
11	27.91178472926103	23.253260166521887	34.81599304354672	14.018962060670365
12	27.045314458347608	22.55318654047956	34.877184067789635	15.524314933383193
13	19.90707731450129	29.429080586968638	35.77089507975856	14.89294701877151
14	15.7692132211975	32.91263013156965	35.83262286737203	15.485533779860816
15	14.8750101761389	26.810391023169387	44.01464111553742	14.299957685154288
16	17.963233498328428	24.941425697188524	42.30030837055638	14.795032433926666
17	18.07470135827971	24.096112849131025	35.790039639974914	22.03914615261435
18	19.161691913928205	26.202953808828145	37.829337875544255	16.80601640169939
19	22.214533583942185	27.085213868891966	33.86583241710808	16.834420130057765
20	23.496906006471576	27.60238537641873	32.77052202921603	16.13018658789366
21	20.645082218729286	27.959735590363664	35.23444482117281	16.16073736973424
22	19.98660775390473	25.032899121586745	35.08267497814926	19.89781814635927
23	16.76844296576076	29.608404283013854	34.14830414086102	19.474848610364365
24	17.02438963295226	28.159322103647217	38.65980027034982	16.156487993050703
25	16.797741299736717	29.24769929800297	36.591024779681	17.36353462257931
26	16.728767206620798	32.577466136940856	33.32088340515526	17.37288325128309
27	16.298909207370478	31.592371161135468	35.65634083042661	16.452378801067443
28	15.381401685973747	30.112738199928252	38.643250066424464	15.862610047673535
29	16.411271672939343	27.183620486826488	38.872984789020684	17.53212305121349
30	18.22441360838281	28.51336227680708	36.6974828481738	16.56474126663631
31	21.621365329201446	28.24426490703706	33.71643327897113	16.417936484790363
32	22.088438922143368	28.3804686123146	33.691160670274314	15.839931795267715
33	21.598776537357388	28.810281881284045	32.68311906037783	16.90782252098074
34	18.042137713799562	29.429348968653912	33.89463871799436	18.63387459955216
35	18.223474272484346	29.58241598982297	33.695812619485764	18.498297118206924
36	22.203395744003235	29.192546861678764	31.575642036086606	17.0284153582314
37	18.826438458737655	31.400970289252832	32.825495544416725	16.947095707592787
38	18.502949067418374	32.370767508997496	30.466823103368995	18.659460320215135
39	18.817179290595636	31.843978991081677	30.725364126851495	18.613477591471188
40	20.53477734608087	30.445084186861642	30.335629189549934	18.68450927750756
41	17.668952980423345	29.445362409208713	31.361562911798146	21.524121698569797
42	20.13122075198759	30.03378925417624	29.624104611602498	20.21088538223367
43	19.767116265629877	30.515981682055376	30.17115594675665	19.545746105558095
44	19.037610114768956	32.02330268712689	30.522557033344633	18.416530164759518
45	18.060790240926238	33.736159332838916	28.545389157916677	19.65766126831817
46	20.315196397244257	32.22588612922936	29.19621474471087	18.262702728815515
47	19.03380804089421	31.162065859076353	30.187974532367278	19.616151567662154
48	19.8784946650194	29.66829812911127	31.253628744036337	19.199578461832996
49	19.76291161922722	28.471271082499634	31.611515721351818	20.15430157692132
50	18.311503465254862	32.153288883362215	30.670837914459597	18.864369736923326
51	17.65128451947601	32.645948196967105	29.46871161582772	20.23405566772916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1759.0
1	2386.0
2	3013.0
3	37049.5
4	71086.0
5	47569.0
6	24052.0
7	24235.5
8	24419.0
9	27029.0
10	29639.0
11	31745.0
12	33851.0
13	34539.5
14	35228.0
15	33940.5
16	32653.0
17	30294.5
18	27936.0
19	26442.0
20	24948.0
21	23422.0
22	21896.0
23	23034.0
24	24172.0
25	24448.0
26	27984.0
27	31244.0
28	37026.0
29	42808.0
30	47054.0
31	51300.0
32	57994.0
33	64688.0
34	70564.0
35	76440.0
36	85469.5
37	94499.0
38	102724.5
39	110950.0
40	127108.5
41	143267.0
42	155965.0
43	168663.0
44	172582.0
45	176501.0
46	181368.5
47	186236.0
48	177725.5
49	169215.0
50	158140.5
51	147066.0
52	129081.5
53	111097.0
54	98096.5
55	85096.0
56	73188.5
57	61281.0
58	53303.5
59	45326.0
60	39071.0
61	32816.0
62	27510.5
63	22205.0
64	18466.5
65	14728.0
66	11150.5
67	7573.0
68	5963.0
69	4353.0
70	4056.5
71	3760.0
72	2893.0
73	2026.0
74	1735.0
75	1210.0
76	976.0
77	668.0
78	360.0
79	277.0
80	194.0
81	133.5
82	73.0
83	53.0
84	33.0
85	22.5
86	12.0
87	10.5
88	9.0
89	8.0
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2235622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.174238390273548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81861792278212	21.418359130255684
2	7.131837361922713	3.876044972670948
3	2.9581112563294707	2.4115326139334585
4	1.8039340262987018	1.9608213308386766
5	1.1939668281028877	1.6222569608473316
6	0.8148999080013026	1.3286570618543627
7	0.6106397190100668	1.1615568506464444
8	0.4622321758017029	1.0048645869512245
9	0.3855546098136293	0.9429437591590019
>10	4.418340096658154	27.91608239784728
>50	1.0294375525467592	19.657829639559793
>100	0.369272409000001	13.381369139715071
>500	0.0016611230170255923	0.2851478188180342
>1k	0.0013288984136204738	0.6618390564366025
>5k	0.0	0.0
>10k+	1.6611230170255923E-4	2.3706946804660975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52519	2.349189621501309	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTT	2920	0.1306124201676312	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCT	2582	0.1154935852304191	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2294	0.10261126433717328	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.473028087932575E-5	0.0	0.0	0.03457650711971881	0.0
2	4.473028087932575E-5	0.0	0.0	0.11598561832009167	0.0
3	4.473028087932575E-5	0.0	0.0	0.22915322894478585	0.0
4	4.473028087932575E-5	0.0	0.0	0.3466596768147746	0.0
5	4.473028087932575E-5	0.0	0.0	0.6311889934881657	0.0
6	4.473028087932575E-5	0.0	0.0	1.2163952582323845	0.0
7	4.473028087932575E-5	0.0	0.0	1.650771015851517	0.0
8	4.473028087932575E-5	0.0	0.0	2.3629665480121416	0.0
9	4.473028087932575E-5	0.0	0.0	2.7695200709243335	0.0
10	4.473028087932575E-5	0.0	0.0	3.188955914729771	0.0
11	4.473028087932575E-5	0.0	0.0	3.541609449182375	0.0
12	4.473028087932575E-5	0.0	0.0	3.814553623108021	0.0
13	4.473028087932575E-5	0.0	0.0	3.9604637993363814	0.0
14	4.473028087932575E-5	0.0	0.0	4.041783449974996	0.0
15	4.473028087932575E-5	0.0	0.0	4.116706670447867	0.0
16	4.473028087932575E-5	0.0	0.0	4.241414693539427	0.0
17	4.473028087932575E-5	0.0	0.0	4.3989100125155325	0.0
18	4.473028087932575E-5	0.0	0.0	4.5918764442289435	0.0
19	4.473028087932575E-5	0.0	0.0	4.711351024457623	0.0
20	4.473028087932575E-5	0.0	0.0	4.838474482716666	0.0
21	4.473028087932575E-5	0.0	0.0	4.98420573782151	0.0
22	4.473028087932575E-5	0.0	0.0	5.153465120668879	0.0
23	4.473028087932575E-5	0.0	0.0	5.3268844196380245	0.0
24	4.473028087932575E-5	0.0	0.0	5.462775012949416	0.0
25	4.473028087932575E-5	0.0	0.0	5.58059457278556	0.0
26	4.473028087932575E-5	0.0	0.0	5.695998697454221	0.0
27	4.473028087932575E-5	0.0	0.0	5.81346041504333	0.0
28	4.473028087932575E-5	0.0	0.0	5.943133499312496	0.0
29	4.473028087932575E-5	0.0	0.0	6.083944423520613	0.0
30	4.473028087932575E-5	0.0	0.0	6.265594094171555	0.0
31	4.473028087932575E-5	0.0	0.0	6.408954644389794	0.0
32	4.473028087932575E-5	0.0	0.0	6.561932204997088	0.0
33	4.473028087932575E-5	0.0	0.0	6.708468605157759	0.0
34	4.473028087932575E-5	0.0	0.0	6.863727410089899	0.0
35	4.473028087932575E-5	0.0	0.0	7.040456749844115	0.0
36	4.473028087932575E-5	0.0	0.0	7.201843603256722	0.0
37	4.473028087932575E-5	0.0	0.0	7.37579966559642	0.0
38	4.473028087932575E-5	0.0	0.0	7.556152158101861	0.0
39	4.473028087932575E-5	0.0	0.0	7.782532109632129	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.034718E-4	45.000004	21
CGTTCCG	20	7.034718E-4	45.000004	40
CGTACTC	20	7.034718E-4	45.000004	42
CCGGTCA	20	7.034718E-4	45.000004	31
CGTTTTT	23900	0.0	43.907955	1
ACGCGAG	80	0.0	42.187504	1
CGGGACG	150	0.0	40.5	6
ATACCGG	100	0.0	40.5	2
ACGGGAC	800	0.0	39.9375	5
AGGGCGA	2055	0.0	39.744526	6
TATAGCG	125	0.0	39.600002	1
GGGCGAT	3950	0.0	39.588608	7
ATAGCGG	385	0.0	39.155846	2
GGCGATC	555	0.0	38.91892	8
ACGTTCG	35	6.250939E-6	38.57143	13
CGTTCGA	35	6.250939E-6	38.57143	14
AGTTCGT	35	6.250939E-6	38.57143	28
TAACGGG	735	0.0	38.265305	3
GTTTTTT	27740	0.0	38.219177	2
GGACCGA	1005	0.0	38.0597	8
>>END_MODULE