FastQCFastQC Report
Thu 26 May 2016
SRR1548161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548161_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2789170
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148570.532667424359218No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53600.19217186474829429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA302.16662E-645.00000411
GTCGAAC253.8922437E-545.022
TAGTCGA351.2127566E-745.012
CGTTTTT89300.041.0946241
TAATACG556.184564E-1140.909091
CTCACGA3250.040.15384324
GTATGCG2050.039.5121961
CGGGTAC4100.038.9634176
CGACGGT3450.038.4782628
CGGTCTA3400.038.3823531
ACGGGTA3850.037.9870155
TCGTTAG950.037.8947371
TATGGGA26200.037.786264
CGAACTA301.1403394E-437.50000418
CGACCGT301.1403394E-437.50000432
TAACGGG7900.037.3101233
TACGGGA9450.036.9047624
GTAGGGC12450.036.686754
CGGTAGT8700.036.46551512
TTAGACG1050.036.428571
Produced by FastQC (version 0.11.5)