##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548161_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2789170
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15007439489167	31.0	31.0	33.0	30.0	34.0
2	31.53468379482068	31.0	31.0	34.0	30.0	34.0
3	31.657397720468815	31.0	31.0	34.0	30.0	34.0
4	35.464653283951854	37.0	35.0	37.0	33.0	37.0
5	35.31980445795703	37.0	35.0	37.0	33.0	37.0
6	35.361740948023964	37.0	35.0	37.0	33.0	37.0
7	35.7675312010383	37.0	35.0	37.0	35.0	37.0
8	35.75577860080239	37.0	35.0	37.0	35.0	37.0
9	37.50291843093107	39.0	37.0	39.0	35.0	39.0
10	36.790682532796495	39.0	37.0	39.0	32.0	39.0
11	36.50426937045788	39.0	35.0	39.0	32.0	39.0
12	35.83502654911676	37.0	35.0	39.0	31.0	39.0
13	35.50726345113421	37.0	35.0	39.0	30.0	39.0
14	36.44527332503935	38.0	35.0	40.0	30.0	41.0
15	36.72388739302373	38.0	35.0	41.0	31.0	41.0
16	36.86474470899945	38.0	35.0	41.0	32.0	41.0
17	36.73972221126715	38.0	35.0	40.0	31.0	41.0
18	36.67611906050904	38.0	35.0	40.0	31.0	41.0
19	36.606909940950175	38.0	35.0	40.0	31.0	41.0
20	36.48057881018367	38.0	35.0	40.0	31.0	41.0
21	36.30108526909439	38.0	35.0	40.0	30.0	41.0
22	36.23396243326868	38.0	35.0	40.0	30.0	41.0
23	36.18501884073039	38.0	34.0	40.0	30.0	41.0
24	36.01471297912999	38.0	34.0	40.0	30.0	41.0
25	35.896465615218865	37.0	34.0	40.0	30.0	41.0
26	35.832128912902405	37.0	34.0	40.0	30.0	41.0
27	35.79189077754314	37.0	34.0	40.0	30.0	41.0
28	35.813420121398124	37.0	34.0	40.0	30.0	41.0
29	35.849917000397966	37.0	34.0	40.0	30.0	41.0
30	35.78226712606259	37.0	34.0	40.0	30.0	41.0
31	35.64082074595668	37.0	34.0	40.0	29.0	41.0
32	35.478410423172484	37.0	34.0	40.0	29.0	41.0
33	35.35749273081239	37.0	34.0	40.0	28.0	41.0
34	35.204597425040426	37.0	34.0	40.0	27.0	41.0
35	35.057883169545065	37.0	34.0	40.0	27.0	41.0
36	34.854621984317916	37.0	34.0	40.0	25.0	41.0
37	34.76836263117702	37.0	33.0	40.0	25.0	41.0
38	34.68582732497482	37.0	33.0	40.0	25.0	41.0
39	34.65804630051234	37.0	33.0	40.0	25.0	41.0
40	34.518942194272846	37.0	33.0	40.0	24.0	41.0
41	34.45928788851164	36.0	33.0	40.0	24.0	41.0
42	34.39847696626595	36.0	33.0	40.0	24.0	41.0
43	34.28453554283174	36.0	33.0	40.0	23.0	41.0
44	34.15278344453726	36.0	33.0	40.0	23.0	41.0
45	34.04359433092999	36.0	33.0	40.0	23.0	41.0
46	33.95289207900558	35.0	33.0	40.0	23.0	41.0
47	33.9006095003173	35.0	33.0	40.0	23.0	41.0
48	33.7737821645866	35.0	33.0	40.0	23.0	41.0
49	33.68207961508262	35.0	33.0	39.0	23.0	41.0
50	33.50980829422373	35.0	32.0	39.0	23.0	41.0
51	33.13840927587777	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	4.0
12	5.0
13	13.0
14	41.0
15	114.0
16	285.0
17	746.0
18	1689.0
19	3135.0
20	5328.0
21	8647.0
22	12608.0
23	18269.0
24	25667.0
25	35120.0
26	46491.0
27	55191.0
28	62746.0
29	71802.0
30	84730.0
31	103094.0
32	126035.0
33	160632.0
34	253408.0
35	338304.0
36	214422.0
37	262659.0
38	370116.0
39	527799.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.580301666804104	24.017109032436174	29.372824173499644	14.02976512726008
2	29.56001247683002	25.978660318302577	30.865239479845258	13.596087725022137
3	28.78806239849131	25.457250723333463	31.930215798965282	13.824471079209943
4	24.74008396763195	28.280061810502765	31.55709404589896	15.422760175966326
5	23.153267818024716	32.362745906488314	31.050670988143423	13.433315287343547
6	21.64156361928459	40.992087251763074	27.087018718830336	10.279330410122007
7	88.31555624074545	3.849173768540462	6.347945804665904	1.4873241860481792
8	89.6654560317227	2.667782888816386	5.957399513116806	1.7093615663441097
9	85.23184316481247	5.116826869642224	7.389761111728579	2.261568853816727
10	45.35126937404318	29.145982496585006	14.085982568290925	11.416765561080895
11	38.10398792472313	23.352968804339643	23.598848402929903	14.94419486800733
12	35.095852888135184	22.22320618678675	25.87189020389578	16.809050721182288
13	23.052592706790907	33.29520968603563	27.070813180982157	16.581384426191303
14	17.490041840404135	36.239849130744986	29.679295274221364	16.59081375462951
15	16.595331227569492	25.119623400509827	42.77706271041205	15.50798266150862
16	20.120609356905458	22.552730740686297	40.443680378033605	16.882979524374637
17	20.05711376502687	23.01046547897762	28.677384311461836	28.255036444533676
18	22.52609199152436	24.488324483627746	33.61078743855699	19.374796086290903
19	27.646719274909742	25.59732823743264	27.17926838450148	19.576684103156136
20	28.96022831164827	25.572553842182444	27.098455813019644	18.368762033149647
21	23.277964412352063	26.99566537715521	30.59333063241036	19.13303957808237
22	24.380442927465875	23.155526554494706	28.689000670450348	23.775029847589067
23	20.53754342689761	28.036476801342335	28.51722913985164	22.90875063190842
24	20.486237841364993	25.620345837650625	35.60324397580642	18.290172345177957
25	20.068443300336657	25.98626114578889	33.39208438352628	20.553211170348167
26	18.91404969937293	32.20061882208686	27.84093475836898	21.044396720171235
27	19.12461413251971	31.627150729428465	30.21393461137184	19.03430052667998
28	17.38320002007766	29.100556796466332	35.441403715083695	18.07483946837231
29	18.30397573471678	26.172660684002768	34.63374408874324	20.889619492537207
30	20.38186987526755	28.83997748434122	31.067593585188426	19.710559055202804
31	26.135337752808184	26.906750036749283	26.85182330227272	20.106088908169813
32	27.119322235647164	27.33210955230409	27.91443332604323	17.634134886005516
33	25.643793673386707	27.364233804321714	26.715402790077334	20.27656973221424
34	20.22142787997863	28.801829935070288	28.9045128120552	22.07222937289588
35	20.216695289279606	27.11659741069924	30.82056669188325	21.846140608137905
36	27.037362369450406	26.165382533155025	28.170566871148043	18.626688226246518
37	20.662992933381616	30.491651638300997	29.27584191712947	19.569513511187917
38	20.72372784735244	30.8215705747588	26.208872173442277	22.245829404446486
39	20.76850819419397	29.39784953946873	28.500091425047597	21.333550841289703
40	23.346049183090308	25.99640753342392	27.726743081275075	22.930800202210694
41	19.07578240121613	25.85848119691521	28.69875267552713	26.36698372634153
42	22.88236285346537	26.02985834495567	27.085512894517006	24.002265907061958
43	22.023182523833253	26.46654739582026	28.149700448520527	23.360569631825957
44	20.405174299164266	29.298536840708884	28.543545212375008	21.752743647751842
45	18.782756160434825	33.01207886217047	25.91706493329557	22.288100044099142
46	21.912504436803783	30.397860295356683	27.03782845792834	20.651806809911193
47	21.600619539146056	28.280958134498796	28.267477421598542	21.850944904756613
48	22.55570653635311	26.479669579122106	30.02283833541878	20.941785549106008
49	21.932761359114004	25.54799456469129	30.56866379603968	21.950580280155027
50	20.30808448391457	30.27155031783649	28.395078105673015	21.02528709257593
51	19.57915795738517	31.47947238784298	26.610066794064185	22.331302860707666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2022.0
1	3041.0
2	4060.0
3	14217.5
4	24375.0
5	17587.5
6	10800.0
7	11053.5
8	11307.0
9	12340.5
10	13374.0
11	13779.0
12	14184.0
13	13967.5
14	13751.0
15	13161.0
16	12571.0
17	12439.5
18	12308.0
19	12006.5
20	11705.0
21	12544.0
22	13383.0
23	15538.5
24	17694.0
25	20128.5
26	26428.0
27	30293.0
28	32640.5
29	34988.0
30	44169.0
31	53350.0
32	63647.0
33	73944.0
34	81128.0
35	88312.0
36	93711.0
37	99110.0
38	110069.5
39	121029.0
40	154113.5
41	187198.0
42	216392.5
43	245587.0
44	248706.5
45	251826.0
46	245682.0
47	239538.0
48	227943.0
49	216348.0
50	206391.5
51	196435.0
52	175330.0
53	154225.0
54	140786.0
55	127347.0
56	117797.0
57	108247.0
58	102013.0
59	95779.0
60	88421.0
61	81063.0
62	70954.5
63	60846.0
64	54658.0
65	48470.0
66	39984.5
67	31499.0
68	26766.0
69	22033.0
70	18810.5
71	15588.0
72	12663.5
73	9739.0
74	8038.5
75	4625.5
76	2913.0
77	2217.0
78	1521.0
79	1165.5
80	810.0
81	571.5
82	333.0
83	258.0
84	183.0
85	141.0
86	99.0
87	70.5
88	42.0
89	30.0
90	18.0
91	16.0
92	14.0
93	10.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2789170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.137458733922262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.66882605121265	15.820712471368797
2	7.050854356248967	2.6987086856322127
3	2.0623062022117336	1.18401899524617
4	1.0284236982738382	0.787256643468132
5	0.5845984772539303	0.5593864617180443
6	0.4016905888087128	0.46124022402790055
7	0.28991904063108354	0.3883819573378985
8	0.24110001486665253	0.3691233268206885
9	0.1863300654950071	0.3209295545369758
>10	2.4791382241500477	12.139084294516032
>50	1.5659939047077478	22.02854517914802
>100	1.4366606227674237	42.04079766701703
>500	0.00359165063972166	0.42655534846213594
>1k	1.8903424419587682E-4	0.0438819131273489
>5k	1.8903424419587682E-4	0.19390523855118724
>10k+	1.8903424419587682E-4	0.5374720390214531
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14857	0.532667424359218	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5360	0.19217186474829429	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.170591968219937E-5	0.0	0.0	0.0226232176597339	0.0
2	7.170591968219937E-5	0.0	0.0	0.07317589103568445	0.0
3	7.170591968219937E-5	0.0	0.0	0.11558994252770538	0.0
4	7.170591968219937E-5	0.0	0.0	0.22214493917545364	0.0
5	7.170591968219937E-5	0.0	0.0	0.37735240232757417	0.0
6	7.170591968219937E-5	0.0	0.0	0.6719920263017314	0.0
7	7.170591968219937E-5	0.0	0.0	0.8116751578426556	0.0
8	7.170591968219937E-5	0.0	0.0	1.1809606442059823	0.0
9	7.170591968219937E-5	0.0	0.0	1.3151224199313774	0.0
10	7.170591968219937E-5	0.0	0.0	1.5403148606933246	0.0
11	7.170591968219937E-5	0.0	0.0	1.7814977215444021	0.0
12	7.170591968219937E-5	0.0	0.0	1.97965703058616	0.0
13	7.170591968219937E-5	0.0	0.0	2.0719066962573094	0.0
14	7.170591968219937E-5	0.0	0.0	2.107723803138568	0.0
15	7.170591968219937E-5	0.0	0.0	2.1567706522011925	0.0
16	7.170591968219937E-5	0.0	0.0	2.2557248213626275	0.0
17	7.170591968219937E-5	0.0	0.0	2.3732866766815937	0.0
18	7.170591968219937E-5	0.0	0.0	2.5331191716532158	0.0
19	7.170591968219937E-5	0.0	0.0	2.6196323637497896	0.0
20	7.170591968219937E-5	0.0	0.0	2.709408175191903	0.0
21	7.170591968219937E-5	0.0	0.0	2.8382637128608152	0.0
22	7.170591968219937E-5	0.0	0.0	2.970883811313043	0.0
23	7.170591968219937E-5	0.0	0.0	3.110889619492537	0.0
24	7.170591968219937E-5	0.0	0.0	3.2196674996504338	0.0
25	7.170591968219937E-5	0.0	0.0	3.309945252530323	0.0
26	7.170591968219937E-5	0.0	0.0	3.407572862177637	0.0
27	7.170591968219937E-5	0.0	0.0	3.498460115374825	0.0
28	7.170591968219937E-5	0.0	0.0	3.5956933424638873	0.0
29	7.170591968219937E-5	0.0	0.0	3.7101359902766773	0.0
30	7.170591968219937E-5	0.0	0.0	3.845588472556352	0.0
31	7.170591968219937E-5	0.0	0.0	3.972221126715116	0.0
32	7.170591968219937E-5	0.0	0.0	4.095304337849611	0.0
33	7.170591968219937E-5	0.0	0.0	4.21849510786363	0.0
34	7.170591968219937E-5	0.0	0.0	4.349322558323802	0.0
35	1.0755887952329904E-4	0.0	0.0	4.500944725491813	0.0
36	1.0755887952329904E-4	0.0	0.0	4.641488328068924	0.0
37	1.0755887952329904E-4	0.0	0.0	4.7838962845577715	0.0
38	1.0755887952329904E-4	0.0	0.0	4.940932248661788	0.0
39	1.0755887952329904E-4	0.0	0.0	5.168204161094519	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATA	30	2.16662E-6	45.000004	11
GTCGAAC	25	3.8922437E-5	45.0	22
TAGTCGA	35	1.2127566E-7	45.0	12
CGTTTTT	8930	0.0	41.094624	1
TAATACG	55	6.184564E-11	40.90909	1
CTCACGA	325	0.0	40.153843	24
GTATGCG	205	0.0	39.512196	1
CGGGTAC	410	0.0	38.963417	6
CGACGGT	345	0.0	38.47826	28
CGGTCTA	340	0.0	38.38235	31
ACGGGTA	385	0.0	37.987015	5
TCGTTAG	95	0.0	37.894737	1
TATGGGA	2620	0.0	37.78626	4
CGAACTA	30	1.1403394E-4	37.500004	18
CGACCGT	30	1.1403394E-4	37.500004	32
TAACGGG	790	0.0	37.310123	3
TACGGGA	945	0.0	36.904762	4
GTAGGGC	1245	0.0	36.68675	4
CGGTAGT	870	0.0	36.465515	12
TTAGACG	105	0.0	36.42857	1
>>END_MODULE