FastQCFastQC Report
Thu 26 May 2016
SRR1548160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548160_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3035601
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187730.6184277841521333No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC55000.18118323192013705No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTC253.892324E-545.022
TAGGTCG302.1666856E-644.9999961
TCGATAG600.044.9999961
CGTTTTT109600.042.2901461
CGTTAGG6000.041.2500042
CACGACG5650.041.01769626
CGACGGT5700.040.65789428
CGGTCTA5700.040.65789431
TACGCGG2400.040.3124962
TCGTTAG1350.040.01
TCACGAC5800.039.56896625
CCGCGAA356.251679E-638.57142636
TAGGGAC35400.037.9449165
TACGGGA9500.037.421054
GGGCGAT33250.037.1503757
CGACAAT5700.037.10526320
ATAGGGA37350.037.0481954
GCGATAT2500.036.99
CGAGGGA21450.036.818184
GCGTAAG1650.036.818181
Produced by FastQC (version 0.11.5)