##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548160_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3035601
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.067552685613162	31.0	31.0	33.0	30.0	34.0
2	31.449808785805512	31.0	31.0	34.0	30.0	34.0
3	31.54560398418633	31.0	31.0	34.0	30.0	34.0
4	35.37116142734173	37.0	35.0	37.0	33.0	37.0
5	35.208656539512276	37.0	35.0	37.0	32.0	37.0
6	35.26058661859711	37.0	35.0	37.0	32.0	37.0
7	35.725315678839216	37.0	35.0	37.0	35.0	37.0
8	35.7277171802223	37.0	35.0	37.0	35.0	37.0
9	37.44599043154881	39.0	37.0	39.0	35.0	39.0
10	36.66056573311183	39.0	37.0	39.0	32.0	39.0
11	36.34723074606973	38.0	35.0	39.0	32.0	39.0
12	35.72004719987903	37.0	35.0	39.0	31.0	39.0
13	35.43978342344728	37.0	35.0	39.0	30.0	39.0
14	36.353208804450915	38.0	35.0	40.0	30.0	41.0
15	36.63321002990841	38.0	35.0	40.0	31.0	41.0
16	36.751526962865015	38.0	35.0	40.0	31.0	41.0
17	36.61915251707981	38.0	35.0	40.0	31.0	41.0
18	36.55799362300908	38.0	35.0	40.0	31.0	41.0
19	36.496910167047645	38.0	35.0	40.0	31.0	41.0
20	36.349967601143895	38.0	35.0	40.0	30.0	41.0
21	36.18267651117522	38.0	34.0	40.0	30.0	41.0
22	36.103158155501994	38.0	34.0	40.0	30.0	41.0
23	36.073160800777174	38.0	34.0	40.0	30.0	41.0
24	35.92033505062095	37.0	34.0	40.0	30.0	41.0
25	35.8077978627626	37.0	34.0	40.0	30.0	41.0
26	35.74957248992868	37.0	34.0	40.0	30.0	41.0
27	35.72206887532321	37.0	34.0	40.0	29.0	41.0
28	35.76438471327424	37.0	34.0	40.0	29.0	41.0
29	35.805257673851074	37.0	34.0	40.0	30.0	41.0
30	35.7107182399795	37.0	34.0	40.0	29.0	41.0
31	35.566978664192035	37.0	34.0	40.0	29.0	41.0
32	35.400522664210484	37.0	34.0	40.0	28.0	41.0
33	35.27305564861785	37.0	34.0	40.0	27.0	41.0
34	35.13212210695674	37.0	34.0	40.0	27.0	41.0
35	34.978556799790226	37.0	34.0	40.0	26.0	41.0
36	34.78833944250249	37.0	33.0	40.0	25.0	41.0
37	34.72232944975311	37.0	33.0	40.0	25.0	41.0
38	34.651275974675194	37.0	33.0	40.0	24.0	41.0
39	34.63858458341528	37.0	33.0	40.0	24.0	41.0
40	34.510019597437214	37.0	33.0	40.0	24.0	41.0
41	34.47237565147725	37.0	33.0	40.0	24.0	41.0
42	34.41077994110557	37.0	33.0	40.0	24.0	41.0
43	34.306255005186785	36.0	33.0	40.0	23.0	41.0
44	34.179683364183894	36.0	33.0	40.0	23.0	41.0
45	34.091901076590766	36.0	33.0	40.0	23.0	41.0
46	33.98838648425798	36.0	33.0	40.0	23.0	41.0
47	33.95749013127878	36.0	33.0	40.0	23.0	41.0
48	33.854631422245546	36.0	33.0	40.0	23.0	41.0
49	33.76180169923518	36.0	33.0	40.0	23.0	41.0
50	33.61462293628181	35.0	32.0	39.0	23.0	41.0
51	33.26533592524182	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	10.0
12	7.0
13	15.0
14	55.0
15	128.0
16	355.0
17	836.0
18	1875.0
19	3547.0
20	6105.0
21	9601.0
22	14186.0
23	20549.0
24	28544.0
25	39641.0
26	52682.0
27	62154.0
28	69972.0
29	80380.0
30	95538.0
31	114706.0
32	140348.0
33	176281.0
34	272088.0
35	354269.0
36	234313.0
37	286318.0
38	403347.0
39	567647.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.671652499785054	24.52107506882492	29.06814828431009	14.739124147079934
2	30.458153097195577	25.471694073101176	30.64062108294206	13.429531746761187
3	27.752461538917668	25.79252016322303	33.03474336712895	13.420274930730356
4	25.038830860841067	27.636240731242346	32.41137422210627	14.913554185810321
5	23.43750051472509	31.712863449445432	30.477292634967508	14.37234340086197
6	22.045387387868168	40.570615176368705	26.83847448989508	10.54552294586805
7	87.67762956989407	3.8558756569127492	6.872872950035265	1.5936218231579182
8	89.07418992153448	2.74495890599588	6.411909865624632	1.768941306845004
9	84.03620897476316	5.444490234388512	8.056131224096974	2.4631695667513616
10	43.35606029909728	31.9168428261817	15.359429648362877	9.36766722635814
11	37.24830766625785	21.102411021738366	25.927155775742595	15.722125536261188
12	33.860609480626735	21.192936752886826	28.456374866130297	16.49007890035614
13	23.377084142481174	31.45667035951036	27.93581896962084	17.230426528387625
14	17.562189497236297	34.56518165595545	29.97732574208534	17.89530310472292
15	16.304250789217686	26.28141840775517	41.96780143371939	15.446529369307758
16	20.728053522185558	23.91381476024023	38.72620281782751	16.631928899746708
17	20.206015217414937	23.04584825212536	29.71928787742526	27.028848653034444
18	22.61450039053222	25.75578938075195	33.03408451901287	18.595625709702954
19	26.459735650370387	26.95324583171504	27.573880757056017	19.01313776085856
20	28.125336630209308	26.058760686928224	27.428703574679282	18.38719910818319
21	23.823651395555608	27.30444481998787	30.33965926351981	18.53224452093671
22	22.60003867438441	23.843153299791375	30.04769072088196	23.50911730494225
23	19.30378860726426	28.41127671258509	29.36568409352876	22.919250586621892
24	20.318381763611228	25.810342004762816	35.631790871066386	18.239485360559573
25	19.11654397267625	27.055532001735404	32.860609810050796	20.96731421553755
26	18.44998733364497	32.4377940315608	28.467015263204882	20.64520337158935
27	18.215437404322902	31.952255912420636	30.694185434778813	19.13812124847765
28	16.53184987091518	28.760795638161934	35.962796164581576	18.744558326341306
29	17.7583615237971	26.271140377144427	34.8770144692929	21.093483629765572
30	19.425181372650755	29.152810267225505	31.87517068284007	19.546837677283673
31	25.887031925473735	27.064558220925612	27.510236029043345	19.53817382455731
32	26.436972447960056	26.93825703707437	28.007040450968358	18.61773006399721
33	24.79525471232879	27.613312816803	27.73039012702921	19.861042343838996
34	19.674785981425096	28.19777698057156	29.974888004055867	22.152549033947476
35	19.97110292162903	27.564689825836798	30.930448369202672	21.533758883331505
36	25.427221825266233	26.749991187906446	28.04953615445508	19.773250832372238
37	20.199262024225188	30.863838824667667	29.473537530129946	19.463361620977196
38	20.245513161973527	32.17702194721902	26.160980972136983	21.41648391867047
39	20.14339170398218	30.446656197570103	28.570092050964536	20.83986004748318
40	22.900934608995055	27.140259869462426	27.70479387771977	22.254011643822754
41	19.043148292545695	25.267451157118476	29.0754285559927	26.613971994343128
42	21.11792689487189	26.311890133123555	27.12672054067712	25.443462431327436
43	20.939115516169615	26.601882131413184	28.16569107731879	24.293311275098407
44	19.79996712347901	29.039883700130552	29.312284453721027	21.847864722669417
45	19.02305342500546	32.7337815477067	26.47314320953248	21.770021817755364
46	21.89556532627312	30.754338267776298	27.052468357995668	20.29762804795492
47	20.254605265975336	28.257303907858773	28.407488335917662	23.080602490248225
48	21.52703863254756	26.097270359312702	30.9531786292072	21.42251237893254
49	21.047957224944913	24.97732738920563	31.58017143886828	22.394543946981173
50	19.779707543909755	29.558265397856964	29.091932701300337	21.57009435693294
51	18.95792628873162	31.08705656639328	27.078855225044396	22.876161919830704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1509.0
1	2816.5
2	4124.0
3	17410.5
4	30697.0
5	21935.5
6	13174.0
7	13167.5
8	13161.0
9	14085.5
10	15010.0
11	15600.0
12	16190.0
13	15733.0
14	15276.0
15	14330.0
16	13384.0
17	12660.5
18	11937.0
19	11470.0
20	11003.0
21	11842.0
22	12681.0
23	14291.0
24	15901.0
25	18743.0
26	24541.0
27	27497.0
28	35032.5
29	42568.0
30	52932.5
31	63297.0
32	72664.5
33	82032.0
34	89593.0
35	97154.0
36	104819.0
37	112484.0
38	127147.5
39	141811.0
40	173824.0
41	205837.0
42	238932.5
43	272028.0
44	274093.5
45	276159.0
46	274163.0
47	272167.0
48	262497.5
49	252828.0
50	239885.0
51	226942.0
52	201085.0
53	175228.0
54	160291.5
55	145355.0
56	130113.5
57	114872.0
58	104080.5
59	93289.0
60	86438.0
61	79587.0
62	68575.5
63	57564.0
64	48115.5
65	38667.0
66	31496.0
67	24325.0
68	20133.5
69	15942.0
70	12710.0
71	9478.0
72	7725.0
73	5972.0
74	4519.0
75	2436.5
76	1807.0
77	1486.5
78	1166.0
79	806.0
80	446.0
81	330.5
82	215.0
83	149.0
84	83.0
85	63.0
86	43.0
87	29.0
88	15.0
89	20.5
90	26.0
91	15.5
92	5.0
93	4.5
94	4.0
95	6.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3035601.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.024319621278295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18007772404674	17.06755900950573
2	7.9915549963187	3.360340130272557
3	2.4886833551900445	1.5696862288701228
4	1.1771126342673774	0.9899196901232883
5	0.7382186698967407	0.7760272633152006
6	0.4871520286265473	0.6145223972399187
7	0.34746467028798	0.51136457996656
8	0.2592816287452515	0.4360975867732628
9	0.21457047066833734	0.4060078340957319
>10	2.6829754907090506	13.814062382820294
>50	1.236326755138546	18.882687294879865
>100	1.192002445821393	40.13702404870737
>500	0.0037896250620450085	0.5241572257933182
>1k	4.7370313275562606E-4	0.10473849182202286
>5k	1.5790104425187534E-4	0.18258691125867693
>10k+	1.5790104425187534E-4	0.6232189245562076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18773	0.6184277841521333	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5500	0.18118323192013705	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.588481160732258E-5	0.0	0.0	0.04196862499386448	0.0
2	6.588481160732258E-5	0.0	0.0	0.15097504579817966	0.0
3	6.588481160732258E-5	0.0	0.0	0.22835675703098002	0.0
4	6.588481160732258E-5	0.0	0.0	0.33897735571967463	0.0
5	6.588481160732258E-5	0.0	0.0	0.6103239523244326	0.0
6	6.588481160732258E-5	0.0	0.0	0.9191260643279535	0.0
7	6.588481160732258E-5	0.0	0.0	1.114375703526254	0.0
8	6.588481160732258E-5	0.0	0.0	1.584694431185126	0.0
9	6.588481160732258E-5	0.0	0.0	1.7568185015092563	0.0
10	6.588481160732258E-5	0.0	0.0	2.0797858480083518	0.0
11	6.588481160732258E-5	0.0	0.0	2.420344439206602	0.0
12	6.588481160732258E-5	0.0	0.0	2.7001901765087046	0.0
13	6.588481160732258E-5	0.0	0.0	2.8189475494309035	0.0
14	6.588481160732258E-5	0.0	0.0	2.865857535295317	0.0
15	6.588481160732258E-5	0.0	0.0	2.940439142034806	0.0
16	6.588481160732258E-5	0.0	0.0	3.0978379569647	0.0
17	6.588481160732258E-5	0.0	0.0	3.2959206430621153	0.0
18	6.588481160732258E-5	0.0	0.0	3.5393979643569757	0.0
19	6.588481160732258E-5	0.0	0.0	3.679996152327002	0.0
20	6.588481160732258E-5	0.0	0.0	3.831695931052862	0.0
21	6.588481160732258E-5	0.0	0.0	4.025990240482857	0.0
22	6.588481160732258E-5	0.0	0.0	4.236558098379859	0.0
23	6.588481160732258E-5	0.0	0.0	4.458886395148769	0.0
24	6.588481160732258E-5	0.0	0.0	4.628243303385392	0.0
25	6.588481160732258E-5	0.0	0.0	4.777406516864371	0.0
26	6.588481160732258E-5	0.0	0.0	4.922122505559854	0.0
27	6.588481160732258E-5	0.0	0.0	5.064894892312923	0.0
28	6.588481160732258E-5	0.0	0.0	5.223216094605319	0.0
29	9.882721741098385E-5	0.0	0.0	5.4006438922638385	0.0
30	9.882721741098385E-5	0.0	0.0	5.601329028419743	0.0
31	9.882721741098385E-5	0.0	0.0	5.781392218542556	0.0
32	9.882721741098385E-5	0.0	0.0	5.953615116084097	0.0
33	9.882721741098385E-5	0.0	0.0	6.13440303913459	0.0
34	9.882721741098385E-5	0.0	0.0	6.327116113086008	0.0
35	9.882721741098385E-5	0.0	0.0	6.533829709503983	0.0
36	9.882721741098385E-5	0.0	0.0	6.7247309511362	0.0
37	9.882721741098385E-5	0.0	0.0	6.92735968923452	0.0
38	9.882721741098385E-5	0.0	0.0	7.145207818814133	0.0
39	9.882721741098385E-5	0.0	0.0	7.419058038259969	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACTC	25	3.892324E-5	45.0	22
TAGGTCG	30	2.1666856E-6	44.999996	1
TCGATAG	60	0.0	44.999996	1
CGTTTTT	10960	0.0	42.290146	1
CGTTAGG	600	0.0	41.250004	2
CACGACG	565	0.0	41.017696	26
CGACGGT	570	0.0	40.657894	28
CGGTCTA	570	0.0	40.657894	31
TACGCGG	240	0.0	40.312496	2
TCGTTAG	135	0.0	40.0	1
TCACGAC	580	0.0	39.568966	25
CCGCGAA	35	6.251679E-6	38.571426	36
TAGGGAC	3540	0.0	37.944916	5
TACGGGA	950	0.0	37.42105	4
GGGCGAT	3325	0.0	37.150375	7
CGACAAT	570	0.0	37.105263	20
ATAGGGA	3735	0.0	37.048195	4
GCGATAT	250	0.0	36.9	9
CGAGGGA	2145	0.0	36.81818	4
GCGTAAG	165	0.0	36.81818	1
>>END_MODULE