FastQCFastQC Report
Thu 26 May 2016
SRR1548159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548159_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1627321
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116140.7136883257820675No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58770.361145711264096No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAC351.2123382E-745.00000419
TAGCGGA351.2123382E-745.0000043
ATGTCGA351.2123382E-745.00000412
GCGATAA700.045.0000049
ACTCGAA351.2123382E-745.00000434
AACTCCG351.2123382E-745.00000427
TACACGG351.2123382E-745.0000042
TCGTTGA253.891524E-545.024
GCCCATA207.034153E-445.026
ACCGGTC207.034153E-445.015
GTCGCAA253.891524E-545.032
CAAGTCG207.034153E-445.01
CACGGAT253.891524E-545.042
CAACGAC207.034153E-445.018
CGTTGAT253.891524E-545.025
CCGTGCA207.034153E-445.016
TTCGTTA253.891524E-545.021
TACGGAC406.8175723E-945.025
TAACGAC207.034153E-445.032
TTATCGG1100.045.02