##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548159_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1627321
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10858275656739	31.0	31.0	33.0	30.0	34.0
2	31.474320677973186	31.0	31.0	34.0	30.0	34.0
3	31.532406943682286	31.0	31.0	34.0	30.0	34.0
4	35.33292448140226	37.0	35.0	37.0	33.0	37.0
5	35.17903535934213	37.0	35.0	37.0	32.0	37.0
6	35.23329324699921	37.0	35.0	37.0	32.0	37.0
7	35.75002903545152	37.0	35.0	37.0	35.0	37.0
8	35.76587532515097	37.0	35.0	37.0	35.0	37.0
9	37.527256146759	39.0	37.0	39.0	35.0	39.0
10	36.816918727159546	39.0	37.0	39.0	32.0	39.0
11	36.36123727279375	38.0	35.0	39.0	32.0	39.0
12	35.27285704541391	37.0	35.0	39.0	30.0	39.0
13	34.673438737655324	37.0	33.0	39.0	27.0	39.0
14	35.515598950668	37.0	33.0	40.0	27.0	41.0
15	36.000219378967024	37.0	34.0	40.0	30.0	41.0
16	36.2792036727849	37.0	35.0	40.0	31.0	41.0
17	36.19517231081022	37.0	34.0	40.0	31.0	41.0
18	36.11695479871519	37.0	35.0	40.0	31.0	41.0
19	35.988839939999544	37.0	35.0	40.0	30.0	41.0
20	35.81453566936087	36.0	34.0	40.0	30.0	41.0
21	35.59476526143275	36.0	34.0	40.0	30.0	41.0
22	35.5007327994907	36.0	34.0	40.0	30.0	41.0
23	35.5462671470472	35.0	34.0	40.0	30.0	41.0
24	35.349798226655956	35.0	34.0	40.0	29.0	41.0
25	35.27893820579959	35.0	34.0	40.0	29.0	41.0
26	35.162887961256565	35.0	34.0	40.0	29.0	41.0
27	35.12467116198955	35.0	34.0	40.0	29.0	41.0
28	35.17579875144486	36.0	34.0	40.0	29.0	41.0
29	35.329282298944094	36.0	34.0	40.0	29.0	41.0
30	35.27994845516035	36.0	34.0	40.0	29.0	41.0
31	35.042728509003446	35.0	34.0	40.0	29.0	41.0
32	34.80611999722243	35.0	34.0	40.0	27.0	41.0
33	34.67299260563835	35.0	33.0	40.0	27.0	41.0
34	34.552552938234065	35.0	34.0	40.0	26.0	41.0
35	34.40434001650566	35.0	33.0	40.0	25.0	41.0
36	34.16263724243711	35.0	33.0	40.0	24.0	41.0
37	34.05848508069398	35.0	33.0	40.0	23.0	41.0
38	34.04839426271768	35.0	33.0	40.0	24.0	41.0
39	34.067402804978244	35.0	33.0	40.0	23.0	41.0
40	33.91808499982486	35.0	33.0	40.0	23.0	41.0
41	33.90227373701931	35.0	33.0	40.0	23.0	41.0
42	33.8303745849774	35.0	33.0	40.0	23.0	41.0
43	33.69248845187889	35.0	33.0	40.0	23.0	41.0
44	33.560342427830776	35.0	33.0	39.0	23.0	41.0
45	33.45677527666638	35.0	32.0	39.0	23.0	41.0
46	33.36927748120991	35.0	32.0	39.0	23.0	41.0
47	33.34707903357727	35.0	32.0	39.0	23.0	41.0
48	33.28976274502695	35.0	32.0	39.0	23.0	41.0
49	33.244284932106204	35.0	32.0	39.0	23.0	41.0
50	33.05653033421187	35.0	32.0	39.0	22.0	40.0
51	32.663653944120426	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	4.0
13	4.0
14	11.0
15	71.0
16	154.0
17	486.0
18	1077.0
19	2080.0
20	3491.0
21	5647.0
22	8674.0
23	12425.0
24	17452.0
25	24272.0
26	31770.0
27	37449.0
28	41496.0
29	46663.0
30	54888.0
31	66337.0
32	81164.0
33	104549.0
34	175045.0
35	249142.0
36	107422.0
37	126085.0
38	175464.0
39	253956.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.771876599638304	24.537199483076787	30.96014861235122	14.73077530493369
2	29.101695363115205	25.863919902711263	32.194754446111126	12.839630288062404
3	28.139746245516406	24.970979911154593	33.00504325821396	13.884230585115045
4	24.698568997757665	27.922087897839454	32.79273112065782	14.586611983745062
5	22.443881692671575	32.27912624491419	31.445547620905774	13.831444441508467
6	21.519786200755718	39.88696759889414	28.297735972189876	10.295510228160271
7	87.4556402824028	3.8221715322299654	7.224266140484883	1.4979220448823558
8	88.70788246449224	2.928678484453897	6.571536900218211	1.7919021508356372
9	85.42457204202489	4.5123856940333225	7.97814321820956	2.084899045732219
10	53.76400845315706	27.254979195868543	11.57743309402386	7.403579256950533
11	45.90550973040967	19.455841840669418	23.420824778885052	11.217823650035855
12	41.86445083668188	21.086313026133137	24.19485768327208	12.854378453912904
13	20.990388497413846	42.29780110992238	24.309586123450753	12.402224269213018
14	13.050959214561846	45.34698439951306	27.687653511507566	13.914402874417526
15	10.935949330218193	25.76197320626969	51.704427092134864	11.59765037137725
16	13.730665308196723	20.721111569260152	49.719815574186036	15.828407548357085
17	14.847654519298898	20.379937332585275	29.13426422936839	35.63814391874744
18	20.422461210787546	24.09420145134242	36.288906736900714	19.194430600969323
19	26.333526083667575	26.528570577040423	28.157751297992224	18.98015204129978
20	30.14703306846037	25.184398161149524	27.003707320190667	17.66486145019944
21	20.315967163208732	28.53831542762614	30.046008132384454	21.099709276780672
22	20.70255346056494	24.376260123233216	29.445696331578098	25.475490084623747
23	16.52599579308569	30.394679353366666	27.015137148724804	26.06418770482283
24	18.399443010936377	24.74588602986135	39.813472572405814	17.04119838679646
25	14.968036423053595	25.92893473383555	37.74805339573446	21.354975447376393
26	15.459826303476696	37.25288372730395	28.27973091971406	19.00755904950529
27	16.226792378393693	36.19679215102614	31.50982504373753	16.066590426842644
28	12.631189544042018	30.873380236597452	41.30475794265544	15.190672276705088
29	13.487504923736621	25.009878198585284	41.20238109137656	20.300235786301535
30	16.834601163507383	31.47885389545148	34.12934510155034	17.557199839490796
31	28.07921731483831	28.23038601480593	26.675069024488714	17.015327645867043
32	27.751132075355756	29.245367078775487	28.407241103629833	14.596259742238932
33	25.326349257460574	31.182784466002712	26.273796011973054	17.21707026456366
34	17.379607342374367	31.686987386016654	29.10931524880463	21.824090022804352
35	17.599047760091587	29.440964628367727	31.59235332180928	21.367634289731406
36	27.51700494247908	25.39173279273112	30.012271703001435	17.078990561788363
37	17.67831915153802	32.49451091702252	32.85061767162102	16.976552259818437
38	17.074197407886953	33.19990339951368	25.846713709219017	23.879185483380354
39	17.793231943789824	32.66675720401814	30.605086519500457	18.934924332691583
40	22.05225643864978	28.52485772628756	26.80159599734779	22.62128983771487
41	16.00366491921385	24.943019846729687	31.082496938219318	27.970818295837148
42	22.370448116874297	25.07728960666027	27.13465874280489	25.41760353366054
43	22.0496140589349	26.739715151466736	27.743082034829026	23.46758875476934
44	17.749294699693547	31.888115497802833	29.4270153215008	20.935574481002824
45	15.684920184769938	39.94749652957223	23.688442538380567	20.679140747277273
46	22.845154705187237	33.895709574202016	25.74488991415953	17.514245806451218
47	20.043740601884938	30.952405825279712	26.85296877506036	22.150884797774996
48	22.983357309344623	25.4415693031676	30.70408358277193	20.870989804715848
49	20.828281574440446	24.00085785164697	32.53758785144419	22.6332727224684
50	18.412409106746612	32.711001701569636	29.350324859078203	19.526264332605553
51	16.24381421981281	36.48001838604676	24.82749254756744	22.448674846572988
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1786.0
1	2013.0
2	2240.0
3	9513.0
4	16786.0
5	11980.5
6	7175.0
7	7783.0
8	8391.0
9	9729.5
10	11068.0
11	11484.5
12	11901.0
13	11783.0
14	11665.0
15	10867.5
16	10070.0
17	9431.5
18	8793.0
19	8541.0
20	8289.0
21	7826.0
22	7363.0
23	8332.0
24	9301.0
25	10993.0
26	15000.5
27	17316.0
28	21306.0
29	25296.0
30	26089.0
31	26882.0
32	33072.5
33	39263.0
34	42256.5
35	45250.0
36	49570.5
37	53891.0
38	62752.5
39	71614.0
40	104821.5
41	138029.0
42	164198.5
43	190368.0
44	201110.0
45	211852.0
46	190795.0
47	169738.0
48	150033.5
49	130329.0
50	118509.0
51	106689.0
52	92799.5
53	78910.0
54	70810.0
55	62710.0
56	52273.5
57	41837.0
58	37508.0
59	33179.0
60	28277.0
61	23375.0
62	19222.0
63	15069.0
64	11563.0
65	8057.0
66	6326.0
67	4595.0
68	3634.5
69	2674.0
70	2122.5
71	1571.0
72	1088.0
73	605.0
74	450.5
75	244.5
76	193.0
77	146.0
78	99.0
79	65.0
80	31.0
81	45.5
82	60.0
83	41.0
84	22.0
85	11.5
86	1.0
87	1.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1627321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.551857872844362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9213216259144	16.630835009385194
2	8.72029770392815	3.5843663804004473
3	2.8535262308021934	1.7593579659563985
4	1.3294391337076417	1.0928977650622713
5	0.7770296299208325	0.79847012585609
6	0.5192828988732846	0.6403337000065414
7	0.35521476789738926	0.511022639691378
8	0.2639983455715706	0.43405251814823753
9	0.20926502124652369	0.387070647697468
>10	1.7016956746967622	8.283665431305277
>50	0.9167139947008774	13.9543389181793
>100	1.4210868849639664	49.369595862718526
>500	0.009624292130970838	1.2500775708954652
>1k	9.02277387278516E-4	0.222768965857756
>5k	3.007591290928387E-4	0.36326670708824416
>10k+	3.007591290928387E-4	0.7178797917513812
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11614	0.7136883257820675	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5877	0.361145711264096	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.145069104374613E-5	0.0	0.0	0.07085264677343929	0.0
2	6.145069104374613E-5	0.0	0.0	0.28648312164594447	0.0
3	6.145069104374613E-5	0.0	0.0	0.41202688344831784	0.0
4	6.145069104374613E-5	0.0	0.0	0.6448021011220282	0.0
5	6.145069104374613E-5	0.0	0.0	1.0786439798908758	0.0
6	6.145069104374613E-5	0.0	0.0	1.5250217996326478	0.0
7	6.145069104374613E-5	0.0	0.0	1.7928239112012934	0.0
8	6.145069104374613E-5	0.0	0.0	2.439039378217328	0.0
9	6.145069104374613E-5	0.0	0.0	2.6254807748440534	0.0
10	6.145069104374613E-5	0.0	0.0	2.9842913598484873	0.0
11	6.145069104374613E-5	0.0	0.0	3.4959298134787176	0.0
12	6.145069104374613E-5	0.0	0.0	3.8972642766854233	0.0
13	6.145069104374613E-5	0.0	0.0	4.052488722261926	0.0
14	6.145069104374613E-5	0.0	0.0	4.114308117451935	0.0
15	6.145069104374613E-5	0.0	0.0	4.217606729096472	0.0
16	6.145069104374613E-5	0.0	0.0	4.45640411449247	0.0
17	6.145069104374613E-5	0.0	0.0	4.733178026953502	0.0
18	6.145069104374613E-5	0.0	0.0	5.1030497363458105	0.0
19	6.145069104374613E-5	0.0	0.0	5.279105966186143	0.0
20	6.145069104374613E-5	0.0	0.0	5.453011421839944	0.0
21	6.145069104374613E-5	0.0	0.0	5.6884904699195795	0.0
22	6.145069104374613E-5	0.0	0.0	5.94013104974372	0.0
23	6.145069104374613E-5	0.0	0.0	6.217642370497278	0.0
24	6.145069104374613E-5	0.0	0.0	6.41748001777154	0.0
25	6.145069104374613E-5	0.0	0.0	6.581922067004604	0.0
26	6.145069104374613E-5	0.0	0.0	6.745196553107838	0.0
27	6.145069104374613E-5	0.0	0.0	6.892555310230741	0.0
28	6.145069104374613E-5	0.0	0.0	7.0528801631638744	0.0
29	6.145069104374613E-5	0.0	0.0	7.229059294386295	0.0
30	6.145069104374613E-5	0.0	0.0	7.45962228718243	0.0
31	6.145069104374613E-5	0.0	0.0	7.681090577704092	0.0
32	6.145069104374613E-5	0.0	0.0	7.8613254545353985	0.0
33	6.145069104374613E-5	0.0	0.0	8.048319907381519	0.0
34	6.145069104374613E-5	0.0	0.0	8.245207921485681	0.0
35	6.145069104374613E-5	0.0	0.0	8.490396178750228	0.0
36	6.145069104374613E-5	0.0	0.0	8.714322496913638	0.0
37	6.145069104374613E-5	0.0	0.0	8.9338243653219	0.0
38	6.145069104374613E-5	0.0	0.0	9.139745631009493	0.0
39	6.145069104374613E-5	0.0	0.0	9.35359403584173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAC	35	1.2123382E-7	45.000004	19
TAGCGGA	35	1.2123382E-7	45.000004	3
ATGTCGA	35	1.2123382E-7	45.000004	12
GCGATAA	70	0.0	45.000004	9
ACTCGAA	35	1.2123382E-7	45.000004	34
AACTCCG	35	1.2123382E-7	45.000004	27
TACACGG	35	1.2123382E-7	45.000004	2
TCGTTGA	25	3.891524E-5	45.0	24
GCCCATA	20	7.034153E-4	45.0	26
ACCGGTC	20	7.034153E-4	45.0	15
GTCGCAA	25	3.891524E-5	45.0	32
CAAGTCG	20	7.034153E-4	45.0	1
CACGGAT	25	3.891524E-5	45.0	42
CAACGAC	20	7.034153E-4	45.0	18
CGTTGAT	25	3.891524E-5	45.0	25
CCGTGCA	20	7.034153E-4	45.0	16
TTCGTTA	25	3.891524E-5	45.0	21
TACGGAC	40	6.8175723E-9	45.0	25
TAACGAC	20	7.034153E-4	45.0	32
TTATCGG	110	0.0	45.0	2
>>END_MODULE