FastQCFastQC Report
Thu 26 May 2016
SRR1548158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548158_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1573608
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231931.4738740524959202No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG207.034082E-445.01
CGTTTTT124650.043.032491
GCGTAAG850.042.3529431
TACGGGA5750.039.1304364
AAGGGAT28450.039.0685425
ACGGGAT7550.039.0397345
CGCAGTA1800.038.7537
ATAGGGA23150.038.390934
CGGTCTA2000.038.2531
TTAGCGG3900.038.0769232
AGGGATC20900.037.8947376
TAAGGGA21300.037.711274
ACGGGCT1850.037.70275
ATATGCG1200.037.5000041
TATTACG301.1401133E-437.5000041
CCGGTAA301.1401133E-437.50000420
AGTACGG1150.037.1739162
AGGGATT32950.037.0789076
TAGGGAC19150.037.0104455
CGCGTAA552.750312E-936.81818431