FastQCFastQC Report
Thu 26 May 2016
SRR1548157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548157_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3492690
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT862072.4682121802965624No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT34970.10012340058808541No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGA253.8924445E-545.00000444
CGCATCG406.8212103E-945.021
CGACCGT207.035266E-445.016
CGCTAGC351.212884E-745.016
CGCTAAC351.212884E-745.044
CGTTTTT386250.044.3417471
CGACGGT4300.043.95348728
CGGTCTA4450.042.4719131
CGAGGGA24250.040.546394
CACGACG4950.039.54545226
TAGCCCG403.4609184E-739.3751
ACGGGTA1550.039.1935465
TACGGGA15250.039.0983624
CTCCGCG356.2519484E-638.5714261
CTATACG356.2519484E-638.5714261
GTTTTTT450350.038.4950562
TAGGGAT74550.038.420525
TAGGGAC46150.037.930665
AGGGATT86400.037.9166686
ACGGGAT18300.037.8688555
Produced by FastQC (version 0.11.5)