##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548157_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3492690
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16512487509627	31.0	31.0	33.0	30.0	34.0
2	31.53773538447443	31.0	31.0	34.0	30.0	34.0
3	31.423213912485792	31.0	31.0	34.0	30.0	34.0
4	35.336030394910516	37.0	35.0	37.0	33.0	37.0
5	35.2473829627021	37.0	35.0	37.0	33.0	37.0
6	35.31524755990368	37.0	35.0	37.0	33.0	37.0
7	35.82045271696028	37.0	35.0	37.0	35.0	37.0
8	35.84484165499944	37.0	35.0	37.0	35.0	37.0
9	37.58091299256447	39.0	37.0	39.0	35.0	39.0
10	36.88660946147525	39.0	37.0	39.0	32.0	39.0
11	36.55396012815337	39.0	35.0	39.0	32.0	39.0
12	36.177754681921385	38.0	35.0	39.0	32.0	39.0
13	36.009066078008644	38.0	35.0	39.0	31.0	39.0
14	37.01521033930867	39.0	35.0	41.0	31.0	41.0
15	37.231044266739964	39.0	35.0	41.0	32.0	41.0
16	37.292835321772046	39.0	35.0	41.0	32.0	41.0
17	37.15371075016677	39.0	35.0	41.0	32.0	41.0
18	36.97410906779588	39.0	35.0	40.0	32.0	41.0
19	36.79916482711034	38.0	35.0	40.0	31.0	41.0
20	36.549368251977704	38.0	35.0	40.0	31.0	41.0
21	36.42287348719755	38.0	35.0	40.0	31.0	41.0
22	36.349575542060705	38.0	35.0	40.0	31.0	41.0
23	36.28196118178252	38.0	35.0	40.0	31.0	41.0
24	36.11746361686837	38.0	35.0	40.0	30.0	41.0
25	36.021415871434336	37.0	35.0	40.0	30.0	41.0
26	35.93490490137974	37.0	35.0	40.0	30.0	41.0
27	35.86020574399675	37.0	34.0	40.0	30.0	41.0
28	35.85342357896063	37.0	35.0	40.0	30.0	41.0
29	35.825236708668676	37.0	35.0	40.0	30.0	41.0
30	35.68308925212372	37.0	34.0	40.0	29.0	41.0
31	35.46126309520742	37.0	34.0	40.0	29.0	41.0
32	35.14810532855765	37.0	34.0	40.0	26.0	41.0
33	34.840005840770296	37.0	34.0	40.0	24.0	41.0
34	34.51345438616081	37.0	34.0	40.0	23.0	41.0
35	34.20145647051413	37.0	33.0	40.0	21.0	41.0
36	33.98265577534794	37.0	33.0	40.0	20.0	41.0
37	33.870065479615995	37.0	33.0	40.0	19.0	41.0
38	33.76265199602599	37.0	33.0	40.0	18.0	41.0
39	33.71390819110771	37.0	33.0	40.0	18.0	41.0
40	33.606371020617345	37.0	33.0	40.0	18.0	41.0
41	33.52689817876765	37.0	33.0	40.0	18.0	41.0
42	33.45609515874584	36.0	33.0	40.0	18.0	41.0
43	33.36427653184222	36.0	33.0	40.0	18.0	41.0
44	33.21653825561387	36.0	32.0	40.0	17.0	41.0
45	33.15935596918135	36.0	32.0	40.0	18.0	41.0
46	33.0486894055871	36.0	32.0	40.0	17.0	41.0
47	33.02872771416874	36.0	32.0	40.0	17.0	41.0
48	32.90111976728539	35.0	32.0	40.0	16.0	41.0
49	32.80695967864311	36.0	31.0	40.0	15.0	41.0
50	32.6805121553874	35.0	31.0	40.0	15.0	41.0
51	32.37114544949595	35.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	6.0
12	6.0
13	18.0
14	26.0
15	125.0
16	420.0
17	996.0
18	2438.0
19	4810.0
20	8135.0
21	12948.0
22	19490.0
23	29210.0
24	43173.0
25	63923.0
26	86105.0
27	94911.0
28	93787.0
29	95596.0
30	105083.0
31	122679.0
32	146483.0
33	182550.0
34	268280.0
35	362592.0
36	286656.0
37	330252.0
38	452990.0
39	678892.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.6897205305939	22.824270118447384	28.37240064248473	20.113608708473983
2	35.81468724679259	23.65809734044533	28.980728321150746	11.546487091611336
3	25.796305999100976	23.68163220898505	38.643452467868606	11.878609324045364
4	23.277559703265965	25.926950287600675	37.90336388285247	12.892126126280889
5	21.551325768963178	30.01434424469392	35.932247064583464	12.502082921759447
6	19.726543151553674	38.0173447972766	32.75346509423968	9.502646956930045
7	82.24365746745346	2.841992847919512	13.631384405715938	1.2829652789110972
8	83.5768132871798	1.941082661215281	13.186168826892738	1.2959352247121847
9	78.99389868554037	5.1663903753267535	14.112589436795133	1.7271215023377398
10	35.359708419584905	38.25423956892825	18.90620123744163	7.47985077404522
11	26.922572573002473	22.049852692337424	36.10787101059641	14.919703724063687
12	26.98550400980333	22.08243502858828	35.32689703351858	15.60516392808981
13	21.150545854341495	28.05479444210623	35.89411027030741	14.900549433244864
14	15.177642447511802	32.34137584497908	36.239460129584934	16.24152157792418
15	14.624859349097688	28.307779963294767	43.129049529159474	13.938311158448071
16	18.5333654003075	26.407153225737183	41.365852680884935	13.693628693070384
17	18.70727147270442	25.185086566514634	35.01109460043691	21.096547360344033
18	18.86256724759426	26.95068843785163	37.96076949285508	16.225974821699037
19	21.202826474722922	27.627702429932228	34.42859228846534	16.74087880687951
20	22.869163882279846	28.4236219074696	33.72463631183987	14.982577898410682
21	20.730296705404715	27.286618623467874	36.68324414706143	15.299840524065978
22	20.296476354901234	23.74579478854407	36.56697273448259	19.3907561220721
23	16.742424893133943	28.350755434922654	36.38098428431953	18.525835387623864
24	16.726448668504794	27.496771829163198	39.822429130555534	15.954350371776481
25	16.79115524137556	29.37936089375238	37.107530298995904	16.721953565876156
26	16.412450002719968	32.718134160203164	33.721429614423265	17.1479862226536
27	15.76933538332918	32.24886835075543	35.71633898227441	16.265457283640977
28	14.331160223209045	30.000286312269342	39.76370648411397	15.904846980407653
29	15.655039525408782	27.84993801339369	38.764390770437686	17.730631690759843
30	17.346114313036658	29.76868831760047	36.691346784283745	16.193850585079122
31	20.94233957207768	29.141864866335116	33.100847770629514	16.814947790957685
32	21.110004037002998	29.039966329677124	34.109125058336126	15.740904574983752
33	20.543306162298975	30.353710177542236	32.541193177751246	16.56179048240754
34	17.347259562114015	31.022621532400528	32.9679988776559	18.662120027829552
35	17.255525111017583	30.816934798106903	33.392542710632775	18.534997380242736
36	21.550638619516764	30.281903060391848	31.333155819726343	16.834302500365048
37	18.36509967961657	32.17666039642797	32.25562532031185	17.20261460364361
38	18.478622494409752	32.93180900681137	30.189538722302867	18.40002977647601
39	18.13521955856374	32.069751395056535	31.13794811449055	18.657080931889176
40	20.101698118069454	30.68609009101867	30.136828633517432	19.075383157394445
41	17.639011764571148	29.65284637342564	31.358351299428232	21.349790562574977
42	19.42136290366451	30.228162247436792	30.232256512888345	20.11821833601035
43	19.73959899103556	30.28516702026232	30.187792217459897	19.78744177124222
44	18.403436892481153	32.02019074123383	30.879808972453898	18.696563393831116
45	17.801522608648348	33.98357712823067	28.91567817355677	19.299222089564203
46	19.390813384525966	32.92193123351915	29.649353363739696	18.03790201821519
47	18.86465732716044	31.53958696592025	30.166805528117298	19.428950178802012
48	19.195462523155506	30.072036166965866	31.806229582356295	18.92627172752234
49	19.206141970801873	29.021499188304716	32.24523218493482	19.527126655958586
50	18.03357870294816	31.954653862781985	31.3343869624845	18.67738047178536
51	17.281579527527494	32.50073725409355	30.221262121745703	19.996421096633256
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2537.0
1	3595.5
2	4654.0
3	61005.5
4	117357.0
5	78346.0
6	39335.0
7	38937.5
8	38540.0
9	42405.0
10	46270.0
11	49030.0
12	51790.0
13	52626.0
14	53462.0
15	51385.0
16	49308.0
17	46031.5
18	42755.0
19	39437.0
20	36119.0
21	35561.0
22	35003.0
23	34224.5
24	33446.0
25	35586.0
26	44625.5
27	51525.0
28	60640.5
29	69756.0
30	78511.5
31	87267.0
32	98969.5
33	110672.0
34	124852.0
35	139032.0
36	148861.0
37	158690.0
38	170564.5
39	182439.0
40	208245.0
41	234051.0
42	260374.5
43	286698.0
44	296254.5
45	305811.0
46	295429.5
47	285048.0
48	263575.0
49	242102.0
50	218996.5
51	195891.0
52	173554.0
53	151217.0
54	135603.5
55	119990.0
56	107477.5
57	94965.0
58	80969.5
59	66974.0
60	57077.0
61	47180.0
62	39344.0
63	31508.0
64	25154.5
65	18801.0
66	14646.5
67	10492.0
68	8032.5
69	5573.0
70	4737.5
71	3902.0
72	2963.0
73	2024.0
74	1743.0
75	1131.5
76	801.0
77	527.5
78	254.0
79	189.5
80	125.0
81	87.0
82	49.0
83	45.0
84	41.0
85	28.5
86	16.0
87	15.5
88	15.0
89	12.5
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3492690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.30588587126792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98245094338321	18.640618733907743
2	7.134518382324264	3.3255254232982465
3	2.733260319511438	1.911031591889966
4	1.5122822357210248	1.409803087634404
5	1.0612868952357801	1.2367115628518683
6	0.7999254413037886	1.1185782624329832
7	0.6022695632896611	0.9825497994037049
8	0.4931854221998527	0.919529853053028
9	0.3752549321945114	0.7871083760120098
>10	3.31614127661825	17.745808421246608
>50	1.1489517476713909	19.44279108971121
>100	0.836099089926746	28.868504846229925
>500	0.0027492146754882108	0.43176386242372805
>1k	0.0014995716411753872	0.6689791514248249
>5k	0.0	0.0
>10k+	1.2496430343128225E-4	2.510695938479751
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	86207	2.4682121802965624	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	3497	0.10012340058808541	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.72624538679356E-5	0.0	0.0	0.036275764525337206	0.0
2	5.72624538679356E-5	0.0	0.0	0.1151547947284185	0.0
3	5.72624538679356E-5	0.0	0.0	0.19612390449767944	0.0
4	5.72624538679356E-5	0.0	0.0	0.28450850204283806	0.0
5	5.72624538679356E-5	0.0	0.0	0.5240087153454787	0.0
6	5.72624538679356E-5	0.0	0.0	0.907838943622251	0.0
7	5.72624538679356E-5	0.0	0.0	1.2257028250431616	0.0
8	5.72624538679356E-5	0.0	0.0	1.6977744947304227	0.0
9	5.72624538679356E-5	0.0	0.0	1.9403668805419318	0.0
10	5.72624538679356E-5	0.0	0.0	2.2263355751584024	0.0
11	5.72624538679356E-5	0.0	0.0	2.5228405612865727	0.0
12	5.72624538679356E-5	0.0	0.0	2.7598784890728925	0.0
13	5.72624538679356E-5	0.0	0.0	2.8838803329239067	0.0
14	5.72624538679356E-5	0.0	0.0	2.9475848128519853	0.0
15	5.72624538679356E-5	0.0	0.0	3.0173877441169985	0.0
16	5.72624538679356E-5	0.0	0.0	3.1369231165663143	0.0
17	5.72624538679356E-5	0.0	0.0	3.2910736423787967	0.0
18	5.72624538679356E-5	0.0	0.0	3.4807555208163334	0.0
19	5.72624538679356E-5	0.0	0.0	3.5945073854249876	0.0
20	5.72624538679356E-5	0.0	0.0	3.7158178939442092	0.0
21	5.72624538679356E-5	0.0	0.0	3.853562726723528	0.0
22	5.72624538679356E-5	0.0	0.0	4.022257915818466	0.0
23	5.72624538679356E-5	0.0	0.0	4.195763151038311	0.0
24	5.72624538679356E-5	0.0	0.0	4.327180482665224	0.0
25	5.72624538679356E-5	0.0	0.0	4.443251476655529	0.0
26	5.72624538679356E-5	0.0	0.0	4.553911168755315	0.0
27	5.72624538679356E-5	0.0	0.0	4.680031723399443	0.0
28	5.72624538679356E-5	0.0	0.0	4.811735367295695	0.0
29	5.72624538679356E-5	0.0	0.0	4.959816072998176	0.0
30	5.72624538679356E-5	0.0	0.0	5.144258436906797	0.0
31	5.72624538679356E-5	0.0	0.0	5.297578657138194	0.0
32	5.72624538679356E-5	0.0	0.0	5.441994565793128	0.0
33	5.72624538679356E-5	0.0	0.0	5.590018009041741	0.0
34	5.72624538679356E-5	0.0	0.0	5.743080548230734	0.0
35	5.72624538679356E-5	0.0	0.0	5.921224042213881	0.0
36	5.72624538679356E-5	0.0	0.0	6.086912952480753	0.0
37	5.72624538679356E-5	0.0	0.0	6.262479636039843	0.0
38	5.72624538679356E-5	0.0	2.86312269339678E-5	6.450701321903748	0.0
39	5.72624538679356E-5	0.0	2.86312269339678E-5	6.689972485390917	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGA	25	3.8924445E-5	45.000004	44
CGCATCG	40	6.8212103E-9	45.0	21
CGACCGT	20	7.035266E-4	45.0	16
CGCTAGC	35	1.212884E-7	45.0	16
CGCTAAC	35	1.212884E-7	45.0	44
CGTTTTT	38625	0.0	44.341747	1
CGACGGT	430	0.0	43.953487	28
CGGTCTA	445	0.0	42.47191	31
CGAGGGA	2425	0.0	40.54639	4
CACGACG	495	0.0	39.545452	26
TAGCCCG	40	3.4609184E-7	39.375	1
ACGGGTA	155	0.0	39.193546	5
TACGGGA	1525	0.0	39.098362	4
CTCCGCG	35	6.2519484E-6	38.571426	1
CTATACG	35	6.2519484E-6	38.571426	1
GTTTTTT	45035	0.0	38.495056	2
TAGGGAT	7455	0.0	38.42052	5
TAGGGAC	4615	0.0	37.93066	5
AGGGATT	8640	0.0	37.916668	6
ACGGGAT	1830	0.0	37.868855	5
>>END_MODULE