FastQCFastQC Report
Thu 26 May 2016
SRR1548156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548156_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences824673
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79940.9693539136118194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTCG302.164752E-645.0000046
GCCGAAT207.0321263E-445.023
TCCGTCG207.0321263E-445.035
ACGTCGG207.0321263E-445.02
TATAGCG551.8189894E-1245.01
TTCGAAG406.8102963E-945.01
CGTTTTT41550.042.8880841
CGCGAGG800.042.18752
CGACGGT750.042.028
TACGGGA3150.041.4285744
ACGGGAT4750.041.2105265
TGTAACG556.002665E-1140.9090921
TACACGG556.002665E-1140.9090922
GCGCGAG501.0804797E-940.51
GTATGCG451.9272193E-840.01
GCGATAA451.9272193E-840.09
CGGTCTA800.039.37531
TAAGGGA13000.038.9423074
ACGGGAC2200.038.8636365
GTACGGG3200.038.6718753