##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548156_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	824673
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263746963948133	31.0	31.0	33.0	30.0	34.0
2	31.666077342170777	31.0	31.0	34.0	30.0	34.0
3	31.70297439106167	31.0	31.0	34.0	30.0	34.0
4	35.537257797939304	37.0	35.0	37.0	33.0	37.0
5	35.416423236846605	37.0	35.0	37.0	33.0	37.0
6	35.46868031328781	37.0	35.0	37.0	33.0	37.0
7	35.87995969311473	37.0	35.0	37.0	35.0	37.0
8	35.89560346949639	37.0	35.0	37.0	35.0	37.0
9	37.658048705365644	39.0	37.0	39.0	35.0	39.0
10	37.009132104482624	39.0	37.0	39.0	33.0	39.0
11	36.63380030630323	39.0	35.0	39.0	32.0	39.0
12	36.192496904833796	38.0	35.0	39.0	32.0	39.0
13	36.018475201686	38.0	35.0	39.0	31.0	39.0
14	37.02708831257965	39.0	35.0	41.0	31.0	41.0
15	37.24598234694236	39.0	35.0	41.0	32.0	41.0
16	37.32926869195427	39.0	35.0	41.0	32.0	41.0
17	37.19631417543681	39.0	35.0	41.0	32.0	41.0
18	37.09494793693985	39.0	35.0	40.0	32.0	41.0
19	37.016373762691394	38.0	35.0	40.0	32.0	41.0
20	36.85699058899709	38.0	35.0	40.0	32.0	41.0
21	36.74051411892956	38.0	35.0	40.0	31.0	41.0
22	36.654140489624375	38.0	35.0	40.0	31.0	41.0
23	36.597565338018825	38.0	35.0	40.0	31.0	41.0
24	36.47178942441428	38.0	35.0	40.0	31.0	41.0
25	36.34723823867157	38.0	35.0	40.0	31.0	41.0
26	36.324785702939224	38.0	35.0	40.0	31.0	41.0
27	36.28766917311468	38.0	35.0	40.0	30.0	41.0
28	36.318306771289954	38.0	35.0	40.0	30.0	41.0
29	36.3309517833129	38.0	35.0	40.0	30.0	41.0
30	36.2603007495092	38.0	35.0	40.0	30.0	41.0
31	36.13707615018316	38.0	35.0	40.0	30.0	41.0
32	35.94014961081544	38.0	35.0	40.0	30.0	41.0
33	35.80096595862845	38.0	35.0	40.0	29.0	41.0
34	35.622021091996466	38.0	34.0	40.0	29.0	41.0
35	35.41949839512146	38.0	34.0	40.0	27.0	41.0
36	35.244125853520124	38.0	34.0	40.0	26.0	41.0
37	35.18555839708588	38.0	34.0	40.0	26.0	41.0
38	35.11993359792306	38.0	34.0	40.0	26.0	41.0
39	35.0740487441689	38.0	34.0	40.0	26.0	41.0
40	34.99589049235273	38.0	34.0	40.0	25.0	41.0
41	34.942646358011	38.0	34.0	40.0	25.0	41.0
42	34.89613337650196	38.0	34.0	40.0	25.0	41.0
43	34.80316198056684	38.0	34.0	40.0	24.0	41.0
44	34.683674620122154	37.0	34.0	40.0	24.0	41.0
45	34.59276828512635	37.0	34.0	40.0	24.0	41.0
46	34.489172071839384	37.0	33.0	40.0	23.0	41.0
47	34.465675485919874	37.0	33.0	40.0	23.0	41.0
48	34.36628578842765	37.0	33.0	40.0	23.0	41.0
49	34.27152337956984	37.0	33.0	40.0	24.0	41.0
50	34.14522240936711	36.0	33.0	40.0	23.0	41.0
51	33.78689977724504	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	3.0
13	5.0
14	8.0
15	21.0
16	70.0
17	139.0
18	319.0
19	600.0
20	1116.0
21	1959.0
22	2999.0
23	4305.0
24	6384.0
25	9473.0
26	13107.0
27	15273.0
28	16644.0
29	18507.0
30	21678.0
31	26670.0
32	33092.0
33	43310.0
34	67167.0
35	89749.0
36	65701.0
37	82649.0
38	120908.0
39	182793.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.111814015979665	24.249126623522294	29.67964271899286	15.959416641505179
2	32.13807169629659	24.518930533678194	30.296372016544737	13.04662575348047
3	27.61518808060892	24.597749653499022	34.38284022879372	13.40422203709834
4	25.217389195475054	27.79174290900757	33.786725162579586	13.204142732937783
5	22.966800174129627	31.860143353789926	31.790661268163262	13.382395203917188
6	21.315236463422472	39.908424308786635	28.661057170539113	10.115282057251783
7	87.57859175697519	3.136394667947174	7.881063160792218	1.4039504142854198
8	88.93015777162584	2.1893526282538653	7.456167474865795	1.4243221252544949
9	84.35864882201794	5.076193836829871	8.638696792546865	1.9264605486053261
10	42.643690286937975	36.181007502367606	13.113197594682982	8.062104616011437
11	30.253324651104137	23.677748634913478	30.838041259990323	15.230885453992068
12	29.809633636605053	22.74695545992169	30.44879606825978	16.994614835213472
13	22.32715270173754	29.94447496159084	30.99919604497783	16.729176291693797
14	16.712563646439254	33.56724422892468	32.20149077270627	17.518701351929796
15	15.70513403494476	28.034384537871375	40.673818592339025	15.58666283484484
16	19.994228015225428	25.515325468397776	38.92912706005895	15.561319456317838
17	19.698353165436483	25.051020222560943	31.687832631843165	23.56279398015941
18	21.042764829211094	27.043446311447084	33.904105021020456	18.009683838321372
19	24.560522776906726	27.21612081394686	30.206033179211637	18.017323229934775
20	26.13848155571966	27.357267668518308	29.84443530951056	16.65981546625147
21	23.137898294232986	27.35981413238944	32.03597062108254	17.46631695229503
22	21.984592680977794	24.14738932886126	32.273034281466714	21.594983708694233
23	18.588337437990575	28.753578691190327	31.863296118582756	20.794787752236342
24	19.52786134625482	26.793165290969878	36.18367522642308	17.49529813635223
25	18.82940268445796	28.51651503080615	33.43361550578229	19.220466778953597
26	18.787325400492076	33.13567923285957	29.634048889681118	18.442946476967233
27	17.8617464134269	31.885365472132595	32.87739504021594	17.375493074224572
28	16.903912217327353	29.033568456830768	36.6451914880201	17.417327837821777
29	17.412477430448188	26.92824913632434	36.633186729770465	19.026086703457008
30	18.9836456389381	29.214367391681307	33.636483794182666	18.165503175197927
31	23.854303463312124	27.45767110115161	30.469531559781878	18.21849387575439
32	23.81064979694982	28.61146175514416	30.571147594258573	17.006740853647447
33	22.880705443248413	28.45442981642421	30.53525457969401	18.129610160633366
34	20.0258769233381	28.50584413458425	31.363097858181366	20.105181083896284
35	19.547990536855213	28.459280223797794	32.28249257584521	19.710236663501775
36	23.46275432807913	28.08458625418802	30.83440345446013	17.618255963272716
37	19.940752273931604	31.194788722317817	31.038241824335223	17.82621717941536
38	19.892126940011373	31.70068621138318	28.3121916201937	20.094995228411747
39	19.661611329581543	31.02975361143144	30.06549262556189	19.243142433425128
40	21.299957680195668	28.86998846815647	29.314407043761587	20.515646807886277
41	18.201153669393808	28.271569458439888	30.25950892050546	23.26776795166084
42	20.679105536376234	28.537735563065603	28.59412154878358	22.189037351774584
43	21.13164854433212	28.482319658822348	28.64735476970872	21.738677027136816
44	19.616502541007165	30.62656349850183	30.166866139669906	19.5900678208211
45	18.54892787808016	32.67695195550237	28.533006415876354	20.241113750541125
46	20.552146123372538	31.53577236068114	28.878719201428932	19.03336231451739
47	19.54095744616351	29.483443740731175	30.053002826574897	20.922595986530418
48	20.77211209776481	27.576384821620202	31.605982007413846	20.045521073201137
49	20.289739084461356	26.927764095586976	31.997531142646846	20.78496567730482
50	18.945569941055425	30.086349377268323	30.653361999240914	20.31471868243534
51	18.487570224804255	31.535166059759444	29.20078625103526	20.77647746440104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	668.0
1	921.0
2	1174.0
3	6951.5
4	12729.0
5	8892.5
6	5056.0
7	5027.5
8	4999.0
9	5305.0
10	5611.0
11	5742.0
12	5873.0
13	5946.0
14	6019.0
15	5646.5
16	5274.0
17	4928.5
18	4583.0
19	4470.0
20	4357.0
21	4515.5
22	4674.0
23	5181.5
24	5689.0
25	6937.0
26	9965.5
27	11746.0
28	13504.0
29	15262.0
30	18876.0
31	22490.0
32	23842.0
33	25194.0
34	29244.5
35	33295.0
36	35480.0
37	37665.0
38	41096.5
39	44528.0
40	51544.5
41	58561.0
42	65451.5
43	72342.0
44	74228.5
45	76115.0
46	74042.5
47	71970.0
48	66837.5
49	61705.0
50	56715.5
51	51726.0
52	47331.5
53	42937.0
54	38609.0
55	34281.0
56	29762.0
57	25243.0
58	23009.0
59	20775.0
60	18312.5
61	15850.0
62	13685.5
63	11521.0
64	9232.5
65	6944.0
66	5572.0
67	4200.0
68	3150.5
69	2101.0
70	1734.0
71	1367.0
72	1146.5
73	926.0
74	682.0
75	400.5
76	363.0
77	219.0
78	75.0
79	90.0
80	105.0
81	69.5
82	34.0
83	23.5
84	13.0
85	8.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	824673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.800689422512686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.43200807166575	20.484305102676966
2	5.954201057493261	3.19153386602147
3	2.549313252030851	2.0497005812512397
4	1.5139581788996959	1.623004918054546
5	1.1307106588091431	1.5151912596734274
6	0.9020405000288562	1.4505184372680853
7	0.7800811870848443	1.463469953358415
8	0.6893475241652589	1.477999111946493
9	0.611607410311159	1.4752350227030988
>10	8.82004089464674	53.64249356499651
>50	0.5994220916429124	9.862451304324143
>100	0.016360269367684237	0.6963091583787214
>500	4.544519268801177E-4	0.0941485008617722
>1k	0.0	0.0
>5k	4.544519268801177E-4	0.973639218485132
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7994	0.9693539136118194	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2126018433973223E-4	0.0	0.0	0.02716228129210002	0.0
2	1.2126018433973223E-4	0.0	0.0	0.08961127622706212	0.0
3	1.2126018433973223E-4	0.0	0.0	0.15715319890429297	0.0
4	1.2126018433973223E-4	0.0	0.0	0.24215658812644528	0.0
5	1.2126018433973223E-4	0.0	0.0	0.4366579238073758	0.0
6	1.2126018433973223E-4	0.0	0.0	0.7464776947953916	0.0
7	1.2126018433973223E-4	0.0	0.0	0.9546814313067119	0.0
8	1.2126018433973223E-4	0.0	0.0	1.4385095668222434	0.0
9	1.2126018433973223E-4	0.0	0.0	1.657990500477159	0.0
10	1.2126018433973223E-4	0.0	0.0	1.9324022976379729	0.0
11	1.2126018433973223E-4	0.0	0.0	2.204025110558973	0.0
12	1.2126018433973223E-4	0.0	0.0	2.444484056104662	0.0
13	1.2126018433973223E-4	0.0	0.0	2.5548308238538184	0.0
14	1.2126018433973223E-4	0.0	0.0	2.6104892484657554	0.0
15	1.2126018433973223E-4	0.0	0.0	2.6724532026633585	0.0
16	1.2126018433973223E-4	0.0	0.0	2.7888629796295015	0.0
17	1.2126018433973223E-4	0.0	0.0	2.9401956896854875	0.0
18	1.2126018433973223E-4	0.0	0.0	3.116629257899798	0.0
19	1.2126018433973223E-4	0.0	0.0	3.228552408045371	0.0
20	1.2126018433973223E-4	0.0	0.0	3.343022022062078	0.0
21	1.2126018433973223E-4	0.0	0.0	3.4936271710120255	0.0
22	1.2126018433973223E-4	0.0	0.0	3.6696969586733164	0.0
23	1.2126018433973223E-4	0.0	0.0	3.8523147962889532	0.0
24	1.2126018433973223E-4	0.0	0.0	3.9881262027494535	0.0
25	1.2126018433973223E-4	0.0	0.0	4.101746995475782	0.0
26	1.2126018433973223E-4	0.0	0.0	4.213548885437016	0.0
27	1.2126018433973223E-4	0.0	0.0	4.32959488185014	0.0
28	1.2126018433973223E-4	0.0	0.0	4.4557054735634605	0.0
29	2.4252036867946446E-4	0.0	0.0	4.5997625725590625	0.0
30	2.4252036867946446E-4	0.0	0.0	4.776681181510732	0.0
31	2.4252036867946446E-4	0.0	0.0	4.934561941521063	0.0
32	2.4252036867946446E-4	0.0	0.0	5.080074162728742	0.0
33	2.4252036867946446E-4	0.0	0.0	5.2225548793279275	0.0
34	2.4252036867946446E-4	0.0	0.0	5.378859256941842	0.0
35	2.4252036867946446E-4	0.0	0.0	5.555535345524833	0.0
36	2.4252036867946446E-4	0.0	0.0	5.720570456411208	0.0
37	2.4252036867946446E-4	0.0	0.0	5.892517397804948	0.0
38	2.4252036867946446E-4	0.0	0.0	6.068708445650579	0.0
39	2.4252036867946446E-4	0.0	0.0	6.281883849719828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTCG	30	2.164752E-6	45.000004	6
GCCGAAT	20	7.0321263E-4	45.0	23
TCCGTCG	20	7.0321263E-4	45.0	35
ACGTCGG	20	7.0321263E-4	45.0	2
TATAGCG	55	1.8189894E-12	45.0	1
TTCGAAG	40	6.8102963E-9	45.0	1
CGTTTTT	4155	0.0	42.888084	1
CGCGAGG	80	0.0	42.1875	2
CGACGGT	75	0.0	42.0	28
TACGGGA	315	0.0	41.428574	4
ACGGGAT	475	0.0	41.210526	5
TGTAACG	55	6.002665E-11	40.909092	1
TACACGG	55	6.002665E-11	40.909092	2
GCGCGAG	50	1.0804797E-9	40.5	1
GTATGCG	45	1.9272193E-8	40.0	1
GCGATAA	45	1.9272193E-8	40.0	9
CGGTCTA	80	0.0	39.375	31
TAAGGGA	1300	0.0	38.942307	4
ACGGGAC	220	0.0	38.863636	5
GTACGGG	320	0.0	38.671875	3
>>END_MODULE