FastQCFastQC Report
Thu 26 May 2016
SRR1548155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548155_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2280621
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167360.733835214180699No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG551.8189894E-1245.01
GTCGAAC207.034751E-445.036
ATAGGCG406.8193913E-945.015
CGCATAT207.034751E-445.011
TCAATCG207.034751E-445.014
AACGCGC207.034751E-445.027
CGTACTA406.8193913E-945.031
CGTACCG207.034751E-445.020
TAGTCGA207.034751E-445.022
TCGCGAC253.8920174E-545.025
TACGTCG207.034751E-445.01
CGTAGTA302.1664455E-644.99999643
CGCACGG1200.044.9999962
CGGTAAG302.1664455E-644.99999635
CGACGGT6200.043.54838628
CACGACG6150.042.43902626
CGGTCTA6100.042.41803431
CGTTTTT81800.041.3966981
TGCGCGA556.184564E-1140.90909216
ACAACGA3250.040.84615313