##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548155_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2280621
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112503129630042	31.0	31.0	33.0	30.0	34.0
2	31.49142273091408	31.0	31.0	34.0	30.0	34.0
3	31.583111792796785	31.0	31.0	34.0	30.0	34.0
4	35.40514140666073	37.0	35.0	37.0	33.0	37.0
5	35.245867244053265	37.0	35.0	37.0	33.0	37.0
6	35.302453586106594	37.0	35.0	37.0	32.0	37.0
7	35.7625690546566	37.0	35.0	37.0	35.0	37.0
8	35.75915156442039	37.0	35.0	37.0	35.0	37.0
9	37.474886883879435	39.0	37.0	39.0	35.0	39.0
10	36.742634571899494	39.0	37.0	39.0	32.0	39.0
11	36.39502705622723	38.0	35.0	39.0	32.0	39.0
12	36.05816398252932	38.0	35.0	39.0	31.0	39.0
13	35.94143788029664	38.0	35.0	39.0	31.0	39.0
14	36.901439564048566	39.0	35.0	41.0	31.0	41.0
15	37.11513443049064	39.0	35.0	41.0	31.0	41.0
16	37.16194624183501	39.0	35.0	41.0	32.0	41.0
17	37.002200277906766	39.0	35.0	41.0	31.0	41.0
18	36.92624596546292	38.0	35.0	40.0	31.0	41.0
19	36.884715610353496	38.0	35.0	40.0	31.0	41.0
20	36.77593865881266	38.0	35.0	40.0	31.0	41.0
21	36.646842680129666	38.0	35.0	40.0	31.0	41.0
22	36.57177847612558	38.0	35.0	40.0	30.0	41.0
23	36.49105046388681	38.0	35.0	40.0	30.0	41.0
24	36.362767421680324	38.0	35.0	40.0	30.0	41.0
25	36.26196417554692	38.0	35.0	40.0	30.0	41.0
26	36.22332864601352	38.0	35.0	40.0	30.0	41.0
27	36.23690608829788	38.0	35.0	40.0	30.0	41.0
28	36.22417797608634	38.0	35.0	40.0	30.0	41.0
29	36.22995403444939	38.0	35.0	40.0	30.0	41.0
30	36.10617985189122	38.0	35.0	40.0	30.0	41.0
31	36.02044574701364	38.0	35.0	40.0	30.0	41.0
32	35.882792011474066	38.0	34.0	40.0	29.0	41.0
33	35.75940412720921	38.0	34.0	40.0	29.0	41.0
34	35.60888459766002	38.0	34.0	40.0	27.0	41.0
35	35.46479401882207	38.0	34.0	40.0	27.0	41.0
36	35.32810098652955	38.0	34.0	40.0	26.0	41.0
37	35.27976152109447	38.0	34.0	40.0	26.0	41.0
38	35.16943981485745	38.0	34.0	40.0	26.0	41.0
39	35.145636210488284	38.0	34.0	40.0	25.0	41.0
40	35.037469619020435	38.0	34.0	40.0	25.0	41.0
41	34.97741360796029	38.0	34.0	40.0	25.0	41.0
42	34.931983437844345	38.0	34.0	40.0	25.0	41.0
43	34.84167382480474	38.0	33.0	40.0	24.0	41.0
44	34.70248980431207	38.0	33.0	40.0	24.0	41.0
45	34.61466898708729	37.0	33.0	40.0	24.0	41.0
46	34.49812266045082	37.0	33.0	40.0	23.0	41.0
47	34.45836989135854	37.0	33.0	40.0	23.0	41.0
48	34.33968248121893	37.0	33.0	40.0	23.0	41.0
49	34.2248712083244	37.0	33.0	40.0	24.0	41.0
50	34.09369246358777	37.0	33.0	40.0	23.0	41.0
51	33.743951318522456	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	6.0
13	9.0
14	37.0
15	87.0
16	193.0
17	524.0
18	1076.0
19	2182.0
20	3700.0
21	5815.0
22	9168.0
23	13578.0
24	19544.0
25	26789.0
26	35413.0
27	41670.0
28	47628.0
29	54469.0
30	65348.0
31	79230.0
32	97459.0
33	123245.0
34	178759.0
35	219186.0
36	184737.0
37	234277.0
38	339479.0
39	496920.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.75365744680944	23.95452817456298	28.242833859725046	14.048980518902527
2	32.204254893732895	24.98455464542333	28.657370075957385	14.153820384886398
3	29.98569249340421	24.90234019593786	31.731664314237218	13.380302996420712
4	27.419856258448906	27.5466199776289	30.174763803367593	14.858759960554604
5	23.34969291258828	32.22082932674916	29.52099450105914	14.908483259603415
6	22.25744654635733	41.41999043243047	25.934997529181747	10.387565492030461
7	87.85159831466956	3.505317192115656	6.999540914514073	1.6435435787007135
8	87.88141475501628	3.0647792859927185	6.984764237459886	2.0690417215311094
9	82.95674730698349	5.910100801492225	8.485583531853823	2.647568359670458
10	39.30907415129476	37.00991966661712	14.188240834404315	9.492765347683811
11	28.369641426611437	24.993631120646526	29.938775447564502	16.697952005177537
12	28.28369992208263	24.018896607546804	30.671909098442924	17.025494371927646
13	23.368240492392204	27.822992071019254	30.743819336926215	18.064948099662328
14	17.479274285381045	32.21249826253463	32.04324611586055	18.264981336223773
15	17.468794683553295	28.6663150080614	37.11945123718496	16.745439071200344
16	22.923580901868394	26.186376429928515	34.79508432133178	16.094958346871312
17	21.862027930112017	25.99195569978528	29.749967223839473	22.396049146263234
18	22.326901313282654	27.348822974093462	32.46396485869419	17.860310853929697
19	23.447780231787746	28.331800855994928	29.313507154411013	18.906911757806316
20	24.734885805225858	28.934969905126717	29.33218627733411	16.997958012313312
21	23.71231344445219	27.37885865297215	31.979623093885394	16.929204808690265
22	22.544561327813785	23.38722654926005	32.78221151168914	21.28600061123703
23	19.908612610337272	28.219989204694684	32.555255783402856	19.316142401565187
24	19.540730353706294	27.22907488793622	34.33827891613731	18.89191584222017
25	20.312800767861035	28.424319516482573	31.690447470228506	19.57243224542789
26	18.78944375238148	32.18369031943492	29.031785640840805	19.99508028734279
27	18.465847679206675	30.981386210159428	32.03408194522457	18.518684165409333
28	17.012646993954718	29.6915182312186	34.73922234338805	18.55661243143863
29	19.630486608691232	27.374430034626535	33.87309859902194	19.121984757660304
30	19.730590922384735	28.189953525815998	33.53932985796412	18.540125693835144
31	23.698106787581104	27.32290897961564	29.88168573384179	19.097298498961468
32	23.554374006027306	28.768874793312875	29.321662827799972	18.35508837285985
33	22.47927209299572	28.349997654147707	29.6281582954818	19.54257195737477
34	20.040944988229086	28.024296891066076	31.400701826388516	20.53405629431633
35	20.00086818458657	28.779091308902267	30.77407425433687	20.445966252174298
36	22.563459689268843	28.696350686940093	29.854850937529736	18.88533868626133
37	20.056335533172764	30.17432532630367	29.859893423764845	19.909445716758725
38	19.785532098494226	30.632577705809076	28.68183709612426	20.900053099572443
39	19.62544412245612	29.085323690345742	30.007835585132298	21.28139660206584
40	21.585524293602486	27.509568665727446	29.959471564981644	20.94543547568842
41	19.376082216203393	27.05986658896853	30.367342929842355	23.196708264985723
42	20.970647906863967	28.539858222826155	28.89072756937694	21.598766300932947
43	20.84940899868939	28.028681661705303	29.117332516012084	22.004576823593222
44	20.585665044740008	28.875337024433257	29.93202290078009	20.60697503004664
45	20.14047928173949	30.24641095561253	28.56748227785327	21.045627484794714
46	21.50892235053523	29.508804838682096	28.47983071277516	20.502442098007517
47	19.589620546333652	29.300440537906127	29.2366421251054	21.873296790654827
48	20.28701831650239	28.4852678283678	30.756579019486356	20.47113483564345
49	20.878480028027454	27.402931043781496	30.510593386625835	21.207995541565214
50	19.551736128010745	29.272071071870336	30.29144255007737	20.884750250041545
51	18.958125878872465	30.21992694095161	29.645478139506743	21.176469040669186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1027.0
1	2301.5
2	3576.0
3	14613.0
4	25650.0
5	17851.5
6	10053.0
7	10002.0
8	9951.0
9	10463.0
10	10975.0
11	11184.5
12	11394.0
13	11345.5
14	11297.0
15	10927.5
16	10558.0
17	9904.0
18	9250.0
19	8973.5
20	8697.0
21	11360.5
22	14024.0
23	15472.0
24	16920.0
25	20366.5
26	28581.5
27	33350.0
28	39946.5
29	46543.0
30	53634.5
31	60726.0
32	67049.0
33	73372.0
34	83742.0
35	94112.0
36	98006.5
37	101901.0
38	114636.0
39	127371.0
40	138492.5
41	149614.0
42	163049.0
43	176484.0
44	179002.0
45	181520.0
46	182904.5
47	184289.0
48	182226.5
49	180164.0
50	175900.5
51	171637.0
52	156246.0
53	140855.0
54	125306.5
55	109758.0
56	97601.0
57	85444.0
58	76143.5
59	66843.0
60	58586.0
61	50329.0
62	40461.0
63	30593.0
64	25151.5
65	19710.0
66	15821.5
67	11933.0
68	9363.5
69	6794.0
70	5356.5
71	3919.0
72	3523.5
73	3128.0
74	2364.0
75	1190.5
76	781.0
77	538.5
78	296.0
79	242.5
80	189.0
81	123.5
82	58.0
83	50.0
84	42.0
85	42.5
86	43.0
87	31.0
88	19.0
89	14.0
90	9.0
91	6.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2280621.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.521286685195804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.98422738230882	16.619005470914434
2	7.670585037395683	3.1482054919114035
3	2.536547648487349	1.5615966445580447
4	1.2660395503246065	1.039230422680305
5	0.7535147173092716	0.7731545767708917
6	0.49694317461656456	0.6118748011534704
7	0.37209455499925714	0.5345101325998078
8	0.27561564058320526	0.4524790060265474
9	0.2334080317073723	0.4310849819964833
>10	2.8484972334504857	14.169748162611082
>50	1.286045344956888	19.207392361785885
>100	1.272177969573547	39.90019579137995
>500	0.00258222857222088	0.3443950844347677
>1k	0.00150630000046218	0.4680840998856952
>5k	0.0	0.0
>10k+	2.1518571435174E-4	0.739042971291226
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16736	0.733835214180699	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3847706392250184E-5	0.0	0.0	0.03428890639873964	0.0
2	4.3847706392250184E-5	0.0	0.0	0.11146086964909996	0.0
3	4.3847706392250184E-5	0.0	0.0	0.16162264576183416	0.0
4	4.3847706392250184E-5	0.0	0.0	0.23519909708802997	0.0
5	4.3847706392250184E-5	0.0	0.0	0.4376001097946568	0.0
6	8.769541278450037E-5	0.0	0.0	0.6325470124146011	0.0
7	8.769541278450037E-5	0.0	0.0	0.7722019572739179	0.0
8	8.769541278450037E-5	0.0	0.0	1.1087331038344381	0.0
9	8.769541278450037E-5	0.0	0.0	1.2210709276113831	0.0
10	1.3154311917675055E-4	0.0	0.0	1.4197887329810608	0.0
11	1.3154311917675055E-4	0.0	0.0	1.7138752997538829	0.0
12	1.3154311917675055E-4	0.0	0.0	1.9524945179405082	0.0
13	1.7539082556900073E-4	0.0	0.0	2.048126365582006	0.0
14	1.7539082556900073E-4	0.0	0.0	2.090921727020842	0.0
15	1.7539082556900073E-4	0.0	0.0	2.1577017838562393	0.0
16	1.7539082556900073E-4	0.0	0.0	2.3060385745812213	0.0
17	1.7539082556900073E-4	0.0	0.0	2.4905497230798104	0.0
18	1.7539082556900073E-4	0.0	0.0	2.7154007614592692	0.0
19	1.7539082556900073E-4	0.0	0.0	2.8542225998971333	0.0
20	1.7539082556900073E-4	0.0	0.0	3.002033218145409	0.0
21	1.7539082556900073E-4	0.0	0.0	3.176547089586564	0.0
22	1.7539082556900073E-4	0.0	0.0	3.3719763169768235	0.0
23	1.7539082556900073E-4	0.0	0.0	3.573631918674782	0.0
24	1.7539082556900073E-4	0.0	0.0	3.727449672698796	0.0
25	1.7539082556900073E-4	0.0	0.0	3.870524738656708	0.0
26	1.7539082556900073E-4	0.0	0.0	4.005444131225662	0.0
27	1.7539082556900073E-4	0.0	0.0	4.150097714613695	0.0
28	1.7539082556900073E-4	0.0	0.0	4.307510980561873	0.0
29	1.7539082556900073E-4	0.0	0.0	4.470098275864337	0.0
30	1.7539082556900073E-4	0.0	0.0	4.725291927067233	0.0
31	1.7539082556900073E-4	0.0	0.0	4.928438350782528	0.0
32	1.7539082556900073E-4	0.0	0.0	5.099312862593127	0.0
33	1.7539082556900073E-4	0.0	0.0	5.285534071641013	0.0
34	1.7539082556900073E-4	0.0	0.0	5.4746053816043965	0.0
35	1.7539082556900073E-4	0.0	0.0	5.689678381458384	0.0
36	1.7539082556900073E-4	0.0	0.0	5.884011416188836	0.0
37	1.7539082556900073E-4	0.0	0.0	6.087727860087231	0.0
38	1.7539082556900073E-4	0.0	0.0	6.320515333323687	0.0
39	1.7539082556900073E-4	0.0	0.0	6.653231729428081	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACG	55	1.8189894E-12	45.0	1
GTCGAAC	20	7.034751E-4	45.0	36
ATAGGCG	40	6.8193913E-9	45.0	15
CGCATAT	20	7.034751E-4	45.0	11
TCAATCG	20	7.034751E-4	45.0	14
AACGCGC	20	7.034751E-4	45.0	27
CGTACTA	40	6.8193913E-9	45.0	31
CGTACCG	20	7.034751E-4	45.0	20
TAGTCGA	20	7.034751E-4	45.0	22
TCGCGAC	25	3.8920174E-5	45.0	25
TACGTCG	20	7.034751E-4	45.0	1
CGTAGTA	30	2.1664455E-6	44.999996	43
CGCACGG	120	0.0	44.999996	2
CGGTAAG	30	2.1664455E-6	44.999996	35
CGACGGT	620	0.0	43.548386	28
CACGACG	615	0.0	42.439026	26
CGGTCTA	610	0.0	42.418034	31
CGTTTTT	8180	0.0	41.396698	1
TGCGCGA	55	6.184564E-11	40.909092	16
ACAACGA	325	0.0	40.846153	13
>>END_MODULE