FastQCFastQC Report
Thu 26 May 2016
SRR1548154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548154_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3073550
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141110.45911080021473544No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC53680.1746514616648501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG406.8212103E-945.00000445
GTCGAAC502.1827873E-1145.019
TCGACTA302.1666947E-644.99999618
TAATGCG1300.043.269231
CGTTTTT92200.041.7299351
GCGATAC2150.040.813959
CGTAAGA501.0822987E-940.520
GGGCGAT30500.040.0573777
GCGAACC451.9301297E-840.038
TATTGCG2250.040.01
TAGGGCG6950.039.4964035
TACGTAG1600.039.3750041
CGGTCTA3450.039.13043631
CGTTAGG4900.039.0306132
GGCGATT9800.038.801028
CGTAAGG5400.038.7500042
CTTGCGG4200.038.571432
CGACGTA356.2517047E-638.57142634
CGCATTA356.2517047E-638.57142628
TACGGGA7000.038.5714264
Produced by FastQC (version 0.11.5)