##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548154_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3073550
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.160443135787606	31.0	31.0	33.0	30.0	34.0
2	31.54388671080672	31.0	31.0	34.0	30.0	34.0
3	31.640146410502513	31.0	31.0	34.0	30.0	34.0
4	35.44480551804916	37.0	35.0	37.0	33.0	37.0
5	35.29577426754079	37.0	35.0	37.0	33.0	37.0
6	35.331258642286606	37.0	35.0	37.0	33.0	37.0
7	35.76986644108604	37.0	35.0	37.0	35.0	37.0
8	35.76391957183062	37.0	35.0	37.0	35.0	37.0
9	37.48140326332742	39.0	37.0	39.0	35.0	39.0
10	36.81497063656033	39.0	37.0	39.0	32.0	39.0
11	36.4786891379675	39.0	35.0	39.0	32.0	39.0
12	35.76000683249012	37.0	35.0	39.0	31.0	39.0
13	35.4215594345301	37.0	35.0	39.0	30.0	39.0
14	36.31150396121748	38.0	35.0	40.0	30.0	41.0
15	36.62429438271705	38.0	35.0	40.0	31.0	41.0
16	36.77266125490068	38.0	35.0	40.0	31.0	41.0
17	36.63936848269916	38.0	35.0	40.0	31.0	41.0
18	36.572665484537424	38.0	35.0	40.0	31.0	41.0
19	36.50477070488523	38.0	35.0	40.0	31.0	41.0
20	36.381042117421224	38.0	35.0	40.0	31.0	41.0
21	36.23463226562119	38.0	34.0	40.0	30.0	41.0
22	36.131051390086384	38.0	34.0	40.0	30.0	41.0
23	36.12293211433033	38.0	34.0	40.0	30.0	41.0
24	35.97645458834247	37.0	34.0	40.0	30.0	41.0
25	35.87025296481268	37.0	34.0	40.0	30.0	41.0
26	35.78698085275984	37.0	34.0	40.0	30.0	41.0
27	35.79044525060598	37.0	34.0	40.0	30.0	41.0
28	35.82268549397277	37.0	34.0	40.0	30.0	41.0
29	35.87967659546778	37.0	34.0	40.0	30.0	41.0
30	35.81507832961884	37.0	34.0	40.0	30.0	41.0
31	35.654429893771045	37.0	34.0	40.0	29.0	41.0
32	35.50228855883262	37.0	34.0	40.0	29.0	41.0
33	35.39725398968619	37.0	34.0	40.0	29.0	41.0
34	35.25741276374225	37.0	34.0	40.0	27.0	41.0
35	35.117607652388934	37.0	34.0	40.0	27.0	41.0
36	34.96907257080574	37.0	34.0	40.0	26.0	41.0
37	34.90688975289161	37.0	34.0	40.0	26.0	41.0
38	34.83523059654146	37.0	34.0	40.0	26.0	41.0
39	34.81484797709489	37.0	34.0	40.0	26.0	41.0
40	34.67319906947992	37.0	34.0	40.0	25.0	41.0
41	34.64392965788746	37.0	34.0	40.0	25.0	41.0
42	34.60362219583218	37.0	33.0	40.0	24.0	41.0
43	34.49191325991118	36.0	33.0	40.0	24.0	41.0
44	34.379955751492574	36.0	33.0	40.0	24.0	41.0
45	34.289753216964094	36.0	33.0	40.0	24.0	41.0
46	34.16959314148135	36.0	33.0	40.0	23.0	41.0
47	34.15122545590604	36.0	33.0	40.0	23.0	41.0
48	34.06115176261977	36.0	33.0	40.0	23.0	41.0
49	33.98214442582681	36.0	33.0	40.0	24.0	41.0
50	33.83462478241773	35.0	33.0	40.0	24.0	41.0
51	33.45458834247043	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	5.0
12	15.0
13	25.0
14	72.0
15	134.0
16	294.0
17	759.0
18	1723.0
19	3191.0
20	5566.0
21	9036.0
22	13497.0
23	19038.0
24	26830.0
25	37320.0
26	48400.0
27	57992.0
28	66383.0
29	77321.0
30	92547.0
31	113629.0
32	140620.0
33	179609.0
34	287048.0
35	380009.0
36	232727.0
37	283455.0
38	404321.0
39	591861.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.894763384360104	23.561744562476612	29.63205413935026	13.911437913813018
2	31.6757495404337	25.286818174423715	29.408501569845946	13.628930715296644
3	28.915553675717003	25.277675651933436	31.92054790063607	13.886222771713491
4	26.019130972328412	27.183192074311464	31.404011647768865	15.393665305591252
5	23.905776707715834	31.634656992728278	29.704641212929673	14.754925086626214
6	22.665940036765303	39.94468936571717	27.23443575019115	10.154934847326382
7	86.27551203006296	4.2412194368076	7.285158855395227	2.198109677734216
8	86.71064404353272	3.583510923850271	7.088318068682793	2.6175269639342127
9	81.49338712563647	6.135153161653462	9.0187242764881	3.352735436221958
10	45.52998975126483	29.50806071155504	14.301312814172537	10.660636723007597
11	39.13861821021295	22.373249174407444	24.08007027704121	14.408062338338404
12	36.454295521465404	21.438076491353648	26.441834360918154	15.665793626262792
13	24.085145841128337	33.113468139447875	26.77831823136113	16.023067788062665
14	16.939109498788046	36.11572936832002	29.48971059524003	17.455450537651902
15	15.674350506743018	27.14697987668982	42.42390720827707	14.754762408290087
16	20.066145011468823	23.396593515641523	40.13824405003986	16.3990174228498
17	19.918823510273135	23.56538855720584	28.800344878072586	27.715443054448443
18	22.075092319955754	25.78074213856941	32.68276097672073	19.461404564754112
19	26.69239153421939	26.589416147451644	27.722731043906883	18.995461274422084
20	28.827544695872852	26.28019716614338	27.453661075954518	17.43859706202925
21	24.21597175904085	26.951928551674776	29.658180280132097	19.173919409152283
22	22.412714938751606	24.33170112736087	29.50578321484928	23.749800719038245
23	20.050690569536854	28.155650632005337	29.13282686144686	22.660831937010947
24	20.53849782824421	25.339135527321826	35.881960599306986	18.24040604512697
25	19.2095785004311	26.431488018740545	33.53949016609458	20.819443314733775
26	18.2454816092141	33.63732491744074	28.33622358510517	19.780969888239984
27	17.991020155845845	32.58782189975761	30.821005026760584	18.60015291763596
28	15.858242097899822	29.73447641977518	36.19030111760017	18.21698036472483
29	17.63189146101414	26.29386214637797	36.31452229506596	19.75972409754193
30	19.5424834474793	28.56000390428007	32.72736737648647	19.170145271754162
31	25.827658570708138	27.254022221860712	27.877600819898813	19.040718387532333
32	26.272030713669857	27.779343104878723	28.592572107172487	17.35605407427893
33	25.31213092352491	27.638658879796978	27.70525939060695	19.343950806071156
34	19.67900310715622	28.342502968879636	29.56922776593841	22.409266158025737
35	19.28444307071627	27.124302516633858	32.05579866929121	21.535455743358657
36	24.930389939971693	26.879862048770963	28.932667436677455	19.25708057457988
37	20.282637341185275	30.3369068341169	30.662523791706658	18.717932032991165
38	20.354476094418505	31.126677620341297	26.55147955946707	21.96736672577313
39	20.573018171170148	29.553350360332516	29.032779684729388	20.840851783767956
40	22.24867010460217	26.550893917457014	28.530819410779067	22.66961656716175
41	18.430934912397717	25.687299702298645	29.556799141058388	26.324966244245253
42	21.886873485057993	25.2100990711067	27.731906102064386	25.17112134177092
43	21.093230954433796	26.250849994306257	28.6197068536383	24.036212197621644
44	19.580354964129427	29.432122464251435	28.94945584096566	22.03806673065348
45	18.66961656716175	32.860828683444225	26.753135624928824	21.716419124465194
46	21.62131736916595	31.0312505083698	27.367929592816125	19.979502529648126
47	20.84319435180817	27.842755120300627	28.863399001155017	22.450651526736184
48	21.435929137316784	25.776154609490654	31.233231930503813	21.554684322688747
49	20.370320964357177	24.824551414488134	32.11742122301573	22.68770639813896
50	19.198191016902282	30.184021733825706	29.763303020936704	20.854484228335313
51	18.71493875160645	30.854419157000862	27.47165329993005	22.95898879146264
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1469.0
1	3147.0
2	4825.0
3	14810.0
4	24795.0
5	18162.5
6	11530.0
7	12098.0
8	12666.0
9	13723.5
10	14781.0
11	15101.0
12	15421.0
13	15508.5
14	15596.0
15	15216.0
16	14836.0
17	13584.0
18	12332.0
19	12530.0
20	12728.0
21	12931.5
22	13135.0
23	15261.0
24	17387.0
25	20760.0
26	29438.0
27	34743.0
28	39239.0
29	43735.0
30	53661.5
31	63588.0
32	73315.5
33	83043.0
34	92230.5
35	101418.0
36	102640.5
37	103863.0
38	120958.5
39	138054.0
40	177615.5
41	217177.0
42	245574.5
43	273972.0
44	287858.0
45	301744.0
46	292329.5
47	282915.0
48	271125.0
49	259335.0
50	241279.5
51	223224.0
52	202984.5
53	182745.0
54	162373.5
55	142002.0
56	124109.5
57	106217.0
58	98685.5
59	91154.0
60	81884.5
61	72615.0
62	66003.5
63	59392.0
64	49320.5
65	39249.0
66	30937.5
67	22626.0
68	19174.5
69	15723.0
70	11472.5
71	7222.0
72	6315.0
73	5408.0
74	4185.5
75	2532.5
76	2102.0
77	1514.5
78	927.0
79	713.5
80	500.0
81	302.5
82	105.0
83	80.0
84	55.0
85	43.0
86	31.0
87	20.5
88	10.0
89	12.0
90	14.0
91	13.0
92	12.0
93	13.5
94	15.0
95	15.0
96	15.0
97	9.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3073550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.95018079677281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.14217313543868	15.187086703628317
2	8.92591574145763	3.3829543415476495
3	2.991071824819679	1.7004405556939828
4	1.4255548289869697	1.0805808698006247
5	0.7910717423772236	0.7495476270633233
6	0.5292800952532248	0.6017972098309046
7	0.34088925469461523	0.4521939105698064
8	0.26404276227521634	0.40029264665557224
9	0.20506745228142317	0.34974587666398943
>10	1.917772833677491	8.588853488987258
>50	0.9123084441491378	12.73216523830455
>100	1.5465565813772737	53.01150261575748
>500	0.006912752676285916	0.8571848438397818
>1k	0.0010369129014428872	0.2677269847325673
>5k	1.7281881690714787E-4	0.17579919927148885
>10k+	1.7281881690714787E-4	0.46212788765275314
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14111	0.45911080021473544	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5368	0.1746514616648501	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.507133445039124E-5	0.0	0.0	0.05469245660555384	0.0
2	6.507133445039124E-5	0.0	0.0	0.22189325047583414	0.0
3	6.507133445039124E-5	0.0	0.0	0.3269509199459908	0.0
4	6.507133445039124E-5	0.0	0.0	0.49350100047176715	0.0
5	6.507133445039124E-5	0.0	0.0	0.8306681199264694	0.0
6	6.507133445039124E-5	0.0	0.0	1.1873241040490639	0.0
7	6.507133445039124E-5	0.0	0.0	1.4172536643295213	0.0
8	6.507133445039124E-5	0.0	0.0	1.9687982951310374	0.0
9	6.507133445039124E-5	0.0	0.0	2.1520717086105643	0.0
10	6.507133445039124E-5	0.0	0.0	2.4690341787184202	0.0
11	6.507133445039124E-5	0.0	0.0	2.883050544159034	0.0
12	6.507133445039124E-5	0.0	0.0	3.2308242911291503	0.0
13	6.507133445039124E-5	0.0	0.0	3.371931479884824	0.0
14	6.507133445039124E-5	0.0	0.0	3.4352133526378292	0.0
15	6.507133445039124E-5	0.0	0.0	3.5313887849555075	0.0
16	6.507133445039124E-5	0.0	0.0	3.740528053879065	0.0
17	6.507133445039124E-5	0.0	0.0	3.9801207073254057	0.0
18	6.507133445039124E-5	0.0	0.0	4.2640920108669125	0.0
19	9.760700167558686E-5	0.0	0.0	4.419514893201672	0.0
20	9.760700167558686E-5	0.0	0.0	4.593450570187568	0.0
21	9.760700167558686E-5	0.0	0.0	4.810300792243497	0.0
22	9.760700167558686E-5	0.0	0.0	5.041043744204584	0.0
23	9.760700167558686E-5	0.0	0.0	5.280831611654276	0.0
24	9.760700167558686E-5	0.0	0.0	5.464137560801028	0.0
25	9.760700167558686E-5	0.0	0.0	5.621545118836525	0.0
26	1.626783361259781E-4	0.0	0.0	5.775796717151177	0.0
27	1.626783361259781E-4	0.0	0.0	5.923996681361943	0.0
28	1.626783361259781E-4	0.0	0.0	6.092596508922907	0.0
29	1.626783361259781E-4	0.0	0.0	6.269427860291845	0.0
30	1.626783361259781E-4	0.0	0.0	6.4830895869597045	0.0
31	1.626783361259781E-4	0.0	0.0	6.68276097672073	0.0
32	1.626783361259781E-4	0.0	0.0	6.867042995884238	0.0
33	1.626783361259781E-4	0.0	0.0	7.0504140163654405	0.0
34	1.626783361259781E-4	0.0	0.0	7.247092124741748	0.0
35	1.626783361259781E-4	0.0	0.0	7.4695710172276355	0.0
36	1.626783361259781E-4	0.0	0.0	7.681866245872037	0.0
37	1.626783361259781E-4	0.0	0.0	7.9000178946169735	0.0
38	1.9521400335117373E-4	0.0	0.0	8.118625042703064	0.0
39	1.9521400335117373E-4	0.0	0.0	8.386914154642026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATCG	40	6.8212103E-9	45.000004	45
GTCGAAC	50	2.1827873E-11	45.0	19
TCGACTA	30	2.1666947E-6	44.999996	18
TAATGCG	130	0.0	43.26923	1
CGTTTTT	9220	0.0	41.729935	1
GCGATAC	215	0.0	40.81395	9
CGTAAGA	50	1.0822987E-9	40.5	20
GGGCGAT	3050	0.0	40.057377	7
GCGAACC	45	1.9301297E-8	40.0	38
TATTGCG	225	0.0	40.0	1
TAGGGCG	695	0.0	39.496403	5
TACGTAG	160	0.0	39.375004	1
CGGTCTA	345	0.0	39.130436	31
CGTTAGG	490	0.0	39.030613	2
GGCGATT	980	0.0	38.80102	8
CGTAAGG	540	0.0	38.750004	2
CTTGCGG	420	0.0	38.57143	2
CGACGTA	35	6.2517047E-6	38.571426	34
CGCATTA	35	6.2517047E-6	38.571426	28
TACGGGA	700	0.0	38.571426	4
>>END_MODULE