FastQCFastQC Report
Thu 26 May 2016
SRR1548153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548153_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2309887
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151590.6562658692827831No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT253.8920327E-545.0000044
CGTTTTT87450.041.680961
CGACGGT3450.041.08695628
TAATGCG1600.040.7812541
TACGGGA9700.040.3608254
TCGAGTA451.9297659E-840.00000424
ACGGGAT9650.039.4041445
ATTACGC403.4602454E-739.37500426
CGTATGG2300.039.1304362
CGGTCTA3650.038.83561731
TACGCTA356.25103E-638.57142628
TAGTAGG11150.038.54262
TAAGGGA30050.038.336114
CACGACG3650.038.21917726
TAGGGCG5600.038.169645
CGGGATC4550.038.0769236
TCGTCCC3550.038.0281738
GTAGGGA33550.037.8241424
TCACGAC3650.037.6027425
TCCCGTA301.1402788E-437.50000416
Produced by FastQC (version 0.11.5)