##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548153_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2309887
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10847153994979	31.0	31.0	33.0	30.0	34.0
2	31.48862217069493	31.0	31.0	34.0	30.0	34.0
3	31.57884952813709	31.0	31.0	34.0	30.0	34.0
4	35.40657616584707	37.0	35.0	37.0	33.0	37.0
5	35.25894600038876	37.0	35.0	37.0	33.0	37.0
6	35.290775263032344	37.0	35.0	37.0	32.0	37.0
7	35.73072838628037	37.0	35.0	37.0	35.0	37.0
8	35.71578912734692	37.0	35.0	37.0	35.0	37.0
9	37.42103531471453	39.0	37.0	39.0	35.0	39.0
10	36.74576029043845	39.0	37.0	39.0	32.0	39.0
11	36.438108011344276	39.0	35.0	39.0	32.0	39.0
12	35.91100300577474	37.0	35.0	39.0	31.0	39.0
13	35.682306104151415	37.0	35.0	39.0	30.0	39.0
14	36.60778600857964	38.0	35.0	41.0	31.0	41.0
15	36.84380880969502	38.0	35.0	41.0	31.0	41.0
16	36.92176976622666	38.0	35.0	41.0	31.0	41.0
17	36.77964419904524	38.0	35.0	41.0	31.0	41.0
18	36.70347250752959	38.0	35.0	40.0	31.0	41.0
19	36.63210797757639	38.0	35.0	40.0	31.0	41.0
20	36.51814396115481	38.0	35.0	40.0	31.0	41.0
21	36.36575771888408	38.0	35.0	40.0	30.0	41.0
22	36.27896083228314	38.0	35.0	40.0	30.0	41.0
23	36.23238063160665	38.0	35.0	40.0	30.0	41.0
24	36.09158240208287	38.0	34.0	40.0	30.0	41.0
25	35.97778938969742	38.0	34.0	40.0	30.0	41.0
26	35.90497024313311	38.0	34.0	40.0	30.0	41.0
27	35.89733134131669	38.0	34.0	40.0	30.0	41.0
28	35.89279778621205	38.0	34.0	40.0	30.0	41.0
29	35.918021530923376	38.0	34.0	40.0	30.0	41.0
30	35.83043672699141	38.0	34.0	40.0	29.0	41.0
31	35.69850559789288	38.0	34.0	40.0	29.0	41.0
32	35.53582621141207	38.0	34.0	40.0	28.0	41.0
33	35.41413844053843	38.0	34.0	40.0	27.0	41.0
34	35.25996985999748	38.0	34.0	40.0	27.0	41.0
35	35.09694933128763	38.0	34.0	40.0	26.0	41.0
36	34.93275558501347	38.0	34.0	40.0	25.0	41.0
37	34.85861602753728	38.0	34.0	40.0	25.0	41.0
38	34.77134292716484	37.0	33.0	40.0	24.0	41.0
39	34.75633526661694	37.0	34.0	40.0	24.0	41.0
40	34.6367809334396	37.0	33.0	40.0	24.0	41.0
41	34.557736374117	37.0	33.0	40.0	24.0	41.0
42	34.51423467901244	37.0	33.0	40.0	24.0	41.0
43	34.412114099087965	37.0	33.0	40.0	23.0	41.0
44	34.28175880465148	37.0	33.0	40.0	23.0	41.0
45	34.19536583391309	37.0	33.0	40.0	23.0	41.0
46	34.068169135546455	36.0	33.0	40.0	23.0	41.0
47	34.04391037310483	36.0	33.0	40.0	23.0	41.0
48	33.93709649000146	36.0	33.0	40.0	23.0	41.0
49	33.81356836936179	36.0	33.0	40.0	23.0	41.0
50	33.659869508768175	36.0	32.0	40.0	23.0	41.0
51	33.30711588921882	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	6.0
13	17.0
14	46.0
15	128.0
16	281.0
17	715.0
18	1483.0
19	2799.0
20	4756.0
21	7416.0
22	10920.0
23	15405.0
24	21908.0
25	30000.0
26	39362.0
27	47144.0
28	52616.0
29	59307.0
30	70025.0
31	83957.0
32	102564.0
33	128931.0
34	193363.0
35	244312.0
36	183925.0
37	227690.0
38	322040.0
39	458695.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.79313057305401	23.78627179597963	26.892527643127128	14.528069987839231
2	32.30582275236841	25.21179607487293	28.491869948616532	13.990511224142132
3	28.90210646667997	24.869874586938668	31.91363906546078	14.314379880920583
4	26.387524584535953	27.552256885293524	30.430103290767036	15.630115239403485
5	23.962384307111126	31.658172023133602	29.46066192848395	14.918781741271324
6	22.81267438623621	39.772941273750625	27.266268869429545	10.148115470583626
7	85.87034777025889	4.237739768222428	7.870341709356345	2.021570752162335
8	86.22980258341642	3.691479280155263	7.790943885999618	2.2877742504287006
9	80.8049484671761	6.376978614105365	9.496958076304166	3.3211148424143695
10	41.16829091639548	34.44393600206417	14.791329619154531	9.596443462385823
11	34.00577604012664	22.69561238277024	26.84053375771196	16.458077819391164
12	32.235732743636376	21.872758277785884	29.419361206846915	16.472147771730825
13	24.85939788396575	28.992587083264247	28.64694246948011	17.501072563289892
14	18.43613994970317	32.45946663191749	30.82691923890649	18.27747417947285
15	18.428563821520275	26.222364990148865	39.15403653944976	16.195034648881094
16	22.21186577525221	24.992391402696324	36.40576357198426	16.38997925006721
17	22.445253815446385	24.701381496151107	28.844657768973114	24.008706919429393
18	23.639424785714628	25.387605540877107	32.51397146267328	18.458998210734983
19	25.32634713299828	27.211287824902257	28.1165702045165	19.345794837582964
20	27.319734688320253	27.34506060253164	28.642613253375597	16.69259145577251
21	25.354270576872374	26.435925220584384	30.236327577929135	17.973476624614104
22	23.487252839641073	23.56496226871704	31.146069050131025	21.801715841510863
23	20.656032091613138	27.439307637126838	30.065756463411415	21.83890380784861
24	21.39329759421132	25.30058829717644	34.64160800939613	18.66450609921611
25	20.409613110944388	26.734294794507264	32.27924136548671	20.57685072906164
26	19.91478371019881	31.43738200180355	28.354763674586682	20.29307061341096
27	19.32371583544996	30.41421506766348	31.366426149850618	18.895642947035938
28	17.567179693205773	28.3112117605753	35.21479622163336	18.906812324585577
29	19.53632363834248	26.071535101067717	34.269078963602986	20.12306229698682
30	20.851323030087617	27.95452764572466	31.978187677579033	19.215961646608687
31	25.15270227504636	27.171155991613443	28.2966223023031	19.379519431037103
32	24.829526292844626	27.781488878027368	28.897863834897553	18.491120994230453
33	24.296166868768907	28.48455357339991	27.897122240178852	19.322157317652337
34	20.23679080405232	28.504857596930066	30.02103566105182	21.237315937965796
35	20.415457552685478	28.676814060601235	30.323950911884435	20.583777474828853
36	24.496869327374025	27.85690382256794	28.572869581931933	19.073357268126102
37	20.84894196123014	30.582231944679545	29.13817862085894	19.430647473231375
38	19.951105833315655	31.269668169914805	27.197694086334096	21.581531910435444
39	20.187177987494625	29.724657526537012	28.825306172985954	21.26285831298241
40	22.88964784857441	27.099031251312294	28.182114536338794	21.829206363774507
41	18.99737952549194	27.266831667523128	29.6021840029404	24.133604804044527
42	21.58707330704922	27.43662352314204	27.585721725781394	23.390581444027347
43	21.246147538819	27.473984658123968	28.286621813101682	22.99324598995535
44	19.79798145969911	29.130689076998138	29.24528342728454	21.826046036018212
45	19.80941059021502	31.69462402273358	27.021451698719464	21.47451368833194
46	21.278313614475515	30.07692584096105	28.063537307236242	20.581223237327194
47	20.519098986227466	28.9377359152201	28.70763807926535	21.835527019287092
48	21.401652981293022	27.05755736103108	30.466425413883886	21.07436424379201
49	21.453213945097747	26.24660860033413	30.53049781223064	21.769679642337483
50	19.81161849042832	29.522699595261585	29.519712436149476	21.14596947816062
51	19.25275998349703	30.253687734508222	27.935695555670037	22.557856726324708
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1364.0
1	2627.5
2	3891.0
3	14574.5
4	25258.0
5	18167.0
6	11076.0
7	11146.0
8	11216.0
9	11810.0
10	12404.0
11	12647.0
12	12890.0
13	12625.0
14	12360.0
15	11662.5
16	10965.0
17	10389.0
18	9813.0
19	9675.5
20	9538.0
21	10051.0
22	10564.0
23	12158.5
24	13753.0
25	16557.5
26	22792.0
27	26222.0
28	30128.0
29	34034.0
30	40644.0
31	47254.0
32	56174.0
33	65094.0
34	73047.5
35	81001.0
36	86336.5
37	91672.0
38	101855.0
39	112038.0
40	128417.0
41	144796.0
42	166420.5
43	188045.0
44	195300.5
45	202556.0
46	197335.5
47	192115.0
48	184296.0
49	176477.0
50	164823.0
51	153169.0
52	142946.5
53	132724.0
54	122176.5
55	111629.0
56	103838.0
57	96047.0
58	89356.5
59	82666.0
60	76040.5
61	69415.0
62	59236.0
63	49057.0
64	41746.5
65	34436.0
66	28078.5
67	21721.0
68	17721.0
69	13721.0
70	11006.0
71	8291.0
72	6637.5
73	4984.0
74	4055.0
75	2398.5
76	1671.0
77	1164.0
78	657.0
79	543.0
80	429.0
81	294.5
82	160.0
83	148.5
84	137.0
85	82.0
86	27.0
87	24.0
88	21.0
89	21.5
90	22.0
91	18.0
92	14.0
93	8.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2309887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.68019043348874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74496508163409	17.722464099487148
2	6.721678056612955	2.9145452059994277
3	2.151433332227243	1.3993045304292562
4	1.0599906640107215	0.919231978138905
5	0.6478952276431009	0.7023245958125484
6	0.45268573928020095	0.5888587820471634
7	0.33857182446908934	0.5138211140932507
8	0.2767377684417049	0.47997820159638976
9	0.23871683832284307	0.46578838630676234
>10	3.7572633147881187	21.017832298937712
>50	1.7428661935427512	27.056635331832897
>100	0.8647798111338298	25.126166339555972
>500	0.0018121109199778704	0.26627794441502306
>1k	4.026913155506379E-4	0.165048673415279
>5k	0.0	0.0
>10k+	2.0134565777531896E-4	0.6617225179323499
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15159	0.6562658692827831	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.039179405745822196	0.0
2	0.0	0.0	0.0	0.12143451173152627	0.0
3	0.0	0.0	0.0	0.17806065837852675	0.0
4	0.0	0.0	0.0	0.2533890185970136	0.0
5	0.0	0.0	0.0	0.43448012824869786	0.0
6	0.0	0.0	0.0	0.6315460453260268	0.0
7	0.0	0.0	0.0	0.7721156922394905	0.0
8	0.0	0.0	0.0	1.0739053468849342	0.0
9	0.0	0.0	0.0	1.1921795308601677	0.0
10	0.0	0.0	0.0	1.3923191913716992	0.0
11	0.0	0.0	0.0	1.6508599771330805	0.0
12	0.0	0.0	0.0	1.8644202075685954	0.0
13	0.0	0.0	0.0	1.9510911139809004	0.0
14	0.0	0.0	0.0	1.9937338926103312	0.0
15	0.0	0.0	0.0	2.0566374026088723	0.0
16	0.0	0.0	0.0	2.179110926205481	0.0
17	0.0	0.0	0.0	2.3313694566011236	0.0
18	0.0	0.0	0.0	2.5116813073539963	0.0
19	0.0	0.0	0.0	2.6191757432289977	0.0
20	0.0	0.0	0.0	2.7423419414023282	0.0
21	0.0	0.0	0.0	2.8911370989143625	0.0
22	0.0	0.0	0.0	3.0575954581328006	0.0
23	0.0	0.0	0.0	3.2298116747702377	0.0
24	0.0	0.0	0.0	3.368173421470401	0.0
25	0.0	0.0	0.0	3.4901707312955135	0.0
26	0.0	0.0	0.0	3.614072896206611	0.0
27	0.0	0.0	0.0	3.7392305337880165	0.0
28	0.0	0.0	0.0	3.874215491926661	0.0
29	0.0	0.0	0.0	4.021538715963162	0.0
30	0.0	0.0	0.0	4.205660276888004	0.0
31	4.32921610451074E-5	0.0	0.0	4.368049172968202	0.0
32	4.32921610451074E-5	0.0	0.0	4.519052230693536	0.0
33	4.32921610451074E-5	0.0	0.0	4.671267468928134	0.0
34	4.32921610451074E-5	0.0	0.0	4.843007471794075	0.0
35	4.32921610451074E-5	0.0	0.0	5.03461857657972	0.0
36	4.32921610451074E-5	0.0	0.0	5.207007961861338	0.0
37	4.32921610451074E-5	0.0	0.0	5.381908292483572	0.0
38	4.32921610451074E-5	0.0	0.0	5.579840052781803	0.0
39	4.32921610451074E-5	0.0	0.0	5.855178197028686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	25	3.8920327E-5	45.000004	4
CGTTTTT	8745	0.0	41.68096	1
CGACGGT	345	0.0	41.086956	28
TAATGCG	160	0.0	40.781254	1
TACGGGA	970	0.0	40.360825	4
TCGAGTA	45	1.9297659E-8	40.000004	24
ACGGGAT	965	0.0	39.404144	5
ATTACGC	40	3.4602454E-7	39.375004	26
CGTATGG	230	0.0	39.130436	2
CGGTCTA	365	0.0	38.835617	31
TACGCTA	35	6.25103E-6	38.571426	28
TAGTAGG	1115	0.0	38.5426	2
TAAGGGA	3005	0.0	38.33611	4
CACGACG	365	0.0	38.219177	26
TAGGGCG	560	0.0	38.16964	5
CGGGATC	455	0.0	38.076923	6
TCGTCCC	355	0.0	38.02817	38
GTAGGGA	3355	0.0	37.824142	4
TCACGAC	365	0.0	37.60274	25
TCCCGTA	30	1.1402788E-4	37.500004	16
>>END_MODULE