FastQCFastQC Report
Thu 26 May 2016
SRR1548152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548152_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3006875
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT908723.022140927042195No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT57480.19116192059862813No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50150.1667844522968198No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT47170.15687383080440656No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC35500.11806277281230512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG650.045.0000041
ACGCCCG207.0351077E-445.035
CGACGTG207.0351077E-445.015
GTCCTCG207.0351077E-445.021
GTTCGCA351.2128112E-745.020
GTCCGCC207.0351077E-445.036
CCGGTGA207.0351077E-445.018
GTGCGTA207.0351077E-445.01
GTTGCGC253.8923165E-545.035
TATCCGA406.8212103E-945.034
CGCGGTA302.1666783E-644.99999640
CCGGTAT302.1666783E-644.99999630
TACGTAC302.1666783E-644.99999624
CGTTTTT287750.044.2806241
CGGTCTA6950.043.70503631
CGACGGT6900.043.0434828
CACGACG7000.042.75000426
TCACGAC7100.042.4647925
ACGGTCT7300.041.3013730
GCGCGAA603.6379788E-1241.24999620