FastQCFastQC Report
Thu 26 May 2016
SRR1548151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548151_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3112926
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282720.9082130445760678No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38420.12342085870335497No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.035146E-445.019
CGGTCTA4350.042.93103431
CGTTTTT168950.042.9091451
CGACGGT4750.040.26315728
GTTAGCG2850.038.684211
AATCGCG700.038.5714261
ACGGGAT15200.038.4868435
ATTAGCG2700.038.3333321
TTAGCGG10050.038.283582
TAAGGGA43100.037.900234
TACGGGA10450.037.8947374
TCACGAC5150.037.57281525
CGTCATA5900.037.37288338
GCGTAAG2000.037.1250041
CGTTAGG4100.036.768292
GGGCGAT35650.036.7321177
AGGGACC31150.036.621196
TAGCGGG20650.036.610173
TAGGGAC33200.036.5963865
TATGGGA36000.036.43754
Produced by FastQC (version 0.11.5)