FastQCFastQC Report
Thu 26 May 2016
SRR1548150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548150_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1389320
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246761.7761206921371606No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23840.17159473699363717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG302.1658307E-645.00000430
ACCGCTC351.2121745E-745.00000418
CGTTGAT302.1658307E-645.00000425
CCAATCG302.1658307E-645.00000424
ATCGGTA302.1658307E-645.00000427
CGGACGG750.045.0000042
AGTCCGC207.033795E-445.027
TAGCGTC207.033795E-445.032
AAGCGTC207.033795E-445.045
ATCGTCC207.033795E-445.032
CGCAGAT207.033795E-445.028
CCGACAA253.8912272E-545.025
GGCGCGT207.033795E-445.08
TATACGT207.033795E-445.022
TATCGCC253.8912272E-545.01
ATACCGG253.8912272E-545.02
TCAACGT253.8912272E-545.040
GTCGGTG253.8912272E-545.016
TAGTACG253.8912272E-545.01
CGTTTTT102350.044.1206671