##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548150_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1389320
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.106986871275158	31.0	31.0	33.0	30.0	34.0
2	31.47277661014021	31.0	31.0	34.0	30.0	34.0
3	31.44738505167996	31.0	31.0	34.0	30.0	34.0
4	35.317313505887775	37.0	35.0	37.0	33.0	37.0
5	35.18813160395013	37.0	35.0	37.0	32.0	37.0
6	35.25876687876083	37.0	35.0	37.0	32.0	37.0
7	35.77959577347191	37.0	35.0	37.0	35.0	37.0
8	35.79737785391414	37.0	35.0	37.0	35.0	37.0
9	37.53358621483891	39.0	37.0	39.0	35.0	39.0
10	36.813714622981024	39.0	37.0	39.0	32.0	39.0
11	36.427975556387295	39.0	35.0	39.0	32.0	39.0
12	35.820999481760865	37.0	35.0	39.0	31.0	39.0
13	35.51890853079204	37.0	35.0	39.0	30.0	39.0
14	36.40955503411741	38.0	35.0	41.0	30.0	41.0
15	36.73724699853165	38.0	35.0	41.0	31.0	41.0
16	36.871333458094604	38.0	35.0	41.0	31.0	41.0
17	36.74879293467308	38.0	35.0	40.0	31.0	41.0
18	36.64421299628595	38.0	35.0	40.0	31.0	41.0
19	36.50732804537471	38.0	35.0	40.0	31.0	41.0
20	36.29955445829614	38.0	35.0	40.0	31.0	41.0
21	36.14150735611666	38.0	34.0	40.0	30.0	41.0
22	36.06407163216537	37.0	34.0	40.0	30.0	41.0
23	36.03027812167103	37.0	34.0	40.0	30.0	41.0
24	35.86987375118763	37.0	34.0	40.0	30.0	41.0
25	35.764747502375265	37.0	34.0	40.0	30.0	41.0
26	35.689374658106125	37.0	34.0	40.0	30.0	41.0
27	35.62646618489621	37.0	34.0	40.0	30.0	41.0
28	35.624747358419945	37.0	34.0	40.0	29.0	41.0
29	35.64647885296404	37.0	34.0	40.0	30.0	41.0
30	35.533142832464804	37.0	34.0	40.0	29.0	41.0
31	35.313456942964905	36.0	34.0	40.0	29.0	41.0
32	35.06221748769182	36.0	34.0	40.0	27.0	41.0
33	34.84840209598941	36.0	34.0	40.0	26.0	41.0
34	34.61923746868972	36.0	34.0	40.0	24.0	41.0
35	34.37790429850575	36.0	33.0	40.0	24.0	41.0
36	34.15833069415253	36.0	33.0	40.0	23.0	41.0
37	34.04088906803328	36.0	33.0	40.0	23.0	41.0
38	33.973859154118564	36.0	33.0	40.0	23.0	41.0
39	33.967661877753144	36.0	33.0	40.0	23.0	41.0
40	33.82949932341001	36.0	33.0	40.0	22.0	41.0
41	33.77734071344255	36.0	33.0	40.0	21.0	41.0
42	33.72847292200501	36.0	33.0	40.0	21.0	41.0
43	33.627280252209715	35.0	33.0	40.0	21.0	41.0
44	33.4874190251346	35.0	33.0	40.0	21.0	41.0
45	33.390992715860996	35.0	32.0	40.0	20.0	41.0
46	33.29849062850891	35.0	32.0	40.0	20.0	41.0
47	33.25863731897619	35.0	32.0	40.0	20.0	41.0
48	33.1456237583854	35.0	32.0	39.0	20.0	41.0
49	33.05850272075548	35.0	32.0	39.0	20.0	41.0
50	32.9053256269254	35.0	32.0	39.0	19.0	41.0
51	32.59640039731667	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	5.0
14	18.0
15	58.0
16	131.0
17	365.0
18	882.0
19	1723.0
20	2943.0
21	4837.0
22	7174.0
23	10519.0
24	15412.0
25	21894.0
26	29636.0
27	34001.0
28	35523.0
29	38966.0
30	44244.0
31	52616.0
32	64474.0
33	81035.0
34	123961.0
35	167146.0
36	113169.0
37	127897.0
38	171500.0
39	239143.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.360392134281515	23.25886044971641	29.386174531425446	16.994572884576627
2	32.912575936429334	25.11581205193908	29.616359082140903	12.355252929490685
3	26.778783865488155	24.41626119252584	35.88914001094061	12.915814931045402
4	23.729450378602483	26.753231796850258	35.89784930757493	13.619468516972333
5	22.159041833414907	31.046843059914202	33.677698442403475	13.11641666426741
6	20.258831658653154	39.696470215645064	29.948248063800996	10.096450061900786
7	85.33282469121585	3.30384648605073	9.951702991391471	1.4116258313419514
8	86.95095442374688	2.166671465177209	9.484711945412144	1.397662165663778
9	82.44277776178274	5.4011314887858815	10.48124262229004	1.6748481271413354
10	41.88595859845104	35.65550053263467	14.993522010767856	7.465018858146431
11	33.75672991103561	21.749057092678434	30.396165030374572	14.09804796591138
12	32.522888895286904	22.39397690956727	30.071617769844238	15.011516425301586
13	21.43149166498719	33.457878674459444	30.507946333458097	14.60268332709527
14	15.197002850315261	37.47185673566925	31.5261422854346	15.804998128580888
15	14.014625860133014	28.37604007716005	43.99382431693202	13.615509745774911
16	17.893429879365446	25.330161517864852	41.97600264877782	14.80040595399188
17	17.815262142630928	24.650260559122447	31.26320790026776	26.27126939797887
18	20.469726196988454	27.35489304120001	34.744695246595455	17.430685515216076
19	23.445498517260244	28.727363026516567	29.92953387268592	17.89760458353727
20	25.58841735525293	28.334077102467393	29.379552586877033	16.69795295540264
21	21.45186134223937	28.65560130135606	32.492226412921426	17.400310943483145
22	20.97738461981401	25.657443929404312	31.816140270060174	21.549031180721503
23	16.665778942216335	30.953775947945754	31.3123686407739	21.068076469064003
24	17.602064319235307	28.594492269599513	37.67490570926784	16.12853770189733
25	16.59610456914174	29.61837445656868	35.2168686839605	18.568652290329084
26	16.101690035413007	35.50492327181643	29.98121383122679	18.412172861543777
27	16.655198226470503	33.68417643163562	33.36524342843981	16.29538191345406
28	14.18283764719431	30.73107707367633	38.60082630351539	16.48525897561397
29	15.55019721878329	28.47234618374457	37.786615034693234	18.190841562778914
30	18.26123571243486	31.199507672818356	33.78875996890565	16.75049664584113
31	24.802493306077793	29.292531598191925	29.464414245818098	16.440560849912185
32	25.01626695073849	30.1548239426482	29.510551924682577	15.318357181930727
33	23.12692540235511	30.3244752828722	28.985762819220913	17.56283649555178
34	18.57232315089396	31.00552788414476	30.41300780237814	20.009141162583134
35	18.54813865776063	30.540264301960672	31.44675092851179	19.464846111766906
36	24.62787550744249	28.637390953847923	29.788313707425214	16.94641983128437
37	18.5406529813146	33.34825670111997	31.2274350041747	16.883655313390726
38	18.678274263668555	34.19536175970978	26.978521866812542	20.147842109809115
39	18.41721129761322	32.80079463334581	29.474491117956987	19.307502951083983
40	20.922249733682666	30.41430340022457	28.330406241902512	20.33304062419025
41	17.147741341087727	29.327152851754818	29.552226988742696	23.972878818414763
42	20.34362133993608	29.832220078887513	27.65100912676705	22.17314945440935
43	20.67788558431463	29.58231364984309	28.295281144732677	21.44451962110961
44	19.190251345982208	32.74062131114502	28.802579679267552	19.266547663605216
45	16.815420493478825	36.56378660063916	26.76482019980998	19.855972706072038
46	20.669608153629113	33.70310655572511	27.624305415599	18.002979875046783
47	19.695606483747444	31.564362421904242	28.14477586157257	20.59525523277575
48	20.540120346644404	29.737209570149425	30.418046238447587	19.304623844758588
49	19.957461204042264	28.343578153341202	30.855022600984654	20.843938041631876
50	18.521722857225118	33.29823222871621	29.144041689459595	19.036003224599085
51	17.775818385972993	34.25035269052486	27.34956669449803	20.624262229004117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1161.0
1	1548.5
2	1936.0
3	16917.5
4	31899.0
5	21108.0
6	10317.0
7	10662.5
8	11008.0
9	12643.5
10	14279.0
11	15807.5
12	17336.0
13	17988.0
14	18640.0
15	17581.0
16	16522.0
17	15388.5
18	14255.0
19	13319.0
20	12383.0
21	11171.0
22	9959.0
23	10146.0
24	10333.0
25	11732.0
26	15611.5
27	18092.0
28	20425.5
29	22759.0
30	26174.5
31	29590.0
32	33857.5
33	38125.0
34	42176.5
35	46228.0
36	49432.0
37	52636.0
38	60700.0
39	68764.0
40	81313.5
41	93863.0
42	109319.5
43	124776.0
44	125969.0
45	127162.0
46	121852.0
47	116542.0
48	113481.5
49	110421.0
50	103767.5
51	97114.0
52	88068.5
53	79023.0
54	68605.0
55	58187.0
56	49077.0
57	39967.0
58	35286.5
59	30606.0
60	25730.5
61	20855.0
62	16881.5
63	12908.0
64	10836.0
65	8764.0
66	6720.5
67	4677.0
68	3418.0
69	2159.0
70	1681.5
71	1204.0
72	989.5
73	775.0
74	619.0
75	384.5
76	306.0
77	212.5
78	119.0
79	76.5
80	34.0
81	25.5
82	17.0
83	14.0
84	11.0
85	8.5
86	6.0
87	5.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1389320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.76853305715924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.86471311793531	21.11092680140831
2	7.537185843535095	4.035188168212437
3	3.072761022091891	2.4675991498985157
4	1.6843512406778751	1.8035044746381153
5	1.0777137490699604	1.4424408059067126
6	0.7441841570532073	1.1952430925215778
7	0.5286201588064001	0.9905270336982921
8	0.4273014245285543	0.9150585846291073
9	0.34897650200353636	0.8407430127048113
>10	4.142946829616092	26.397836055553125
>50	1.1841762151694968	22.11578670845145
>100	0.38463873626422446	14.355989055280135
>500	0.0016206688322699973	0.2941362464927976
>1k	5.402229440899991E-4	0.2508256241601561
>5k	0.0	0.0
>10k+	2.7011147204499957E-4	1.7841951864445118
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24676	1.7761206921371606	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2384	0.17159473699363717	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1593297440474478E-4	0.0	0.0	0.04491405867618691	0.0
2	2.1593297440474478E-4	0.0	0.0	0.1597184234013762	0.0
3	2.1593297440474478E-4	0.0	0.0	0.2590475916275588	0.0
4	2.1593297440474478E-4	0.0	0.0	0.36557452566723286	0.0
5	2.1593297440474478E-4	0.0	0.0	0.65564448795094	0.0
6	2.1593297440474478E-4	0.0	0.0	1.0566320214205511	0.0
7	2.1593297440474478E-4	0.0	0.0	1.3543316154665592	0.0
8	2.1593297440474478E-4	0.0	0.0	1.9132381308841735	0.0
9	2.1593297440474478E-4	0.0	0.0	2.170126392767685	0.0
10	2.1593297440474478E-4	0.0	0.0	2.489059395963493	0.0
11	2.1593297440474478E-4	0.0	0.0	2.876443152045605	0.0
12	2.1593297440474478E-4	0.0	0.0	3.1731350588777243	0.0
13	2.1593297440474478E-4	0.0	0.0	3.308525033829499	0.0
14	2.1593297440474478E-4	0.0	0.0	3.3811504908876286	0.0
15	2.1593297440474478E-4	0.0	0.0	3.4648605072985346	0.0
16	2.1593297440474478E-4	0.0	0.0	3.6236432211441567	0.0
17	2.1593297440474478E-4	0.0	0.0	3.8421673912417584	0.0
18	2.1593297440474478E-4	0.0	0.0	4.093513373448881	0.0
19	2.1593297440474478E-4	0.0	0.0	4.244090634267123	0.0
20	2.1593297440474478E-4	0.0	0.0	4.3930843866063976	0.0
21	2.1593297440474478E-4	0.0	0.0	4.591598767742493	0.0
22	2.1593297440474478E-4	0.0	0.0	4.812066334609737	0.0
23	2.1593297440474478E-4	0.0	0.0	5.038939913050989	0.0
24	2.1593297440474478E-4	0.0	0.0	5.2120461808654595	0.0
25	2.1593297440474478E-4	0.0	0.0	5.353338323784297	0.0
26	2.1593297440474478E-4	0.0	0.0	5.49866121555869	0.0
27	2.1593297440474478E-4	0.0	0.0	5.650606051881496	0.0
28	2.1593297440474478E-4	0.0	0.0	5.807301413641206	0.0
29	2.1593297440474478E-4	0.0	0.0	5.988253246192382	0.0
30	2.1593297440474478E-4	0.0	0.0	6.200011516425302	0.0
31	2.1593297440474478E-4	0.0	0.0	6.39593470186854	0.0
32	2.1593297440474478E-4	0.0	0.0	6.560187717732416	0.0
33	2.1593297440474478E-4	0.0	0.0	6.740347796044108	0.0
34	2.1593297440474478E-4	0.0	0.0	6.934039674085164	0.0
35	2.1593297440474478E-4	0.0	0.0	7.157818213226615	0.0
36	2.1593297440474478E-4	0.0	0.0	7.351366135951401	0.0
37	2.1593297440474478E-4	0.0	0.0	7.565139780612098	0.0
38	2.879106325396597E-4	0.0	0.0	7.780209023119224	0.0
39	2.879106325396597E-4	0.0	0.0	8.07567730976305	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	30	2.1658307E-6	45.000004	30
ACCGCTC	35	1.2121745E-7	45.000004	18
CGTTGAT	30	2.1658307E-6	45.000004	25
CCAATCG	30	2.1658307E-6	45.000004	24
ATCGGTA	30	2.1658307E-6	45.000004	27
CGGACGG	75	0.0	45.000004	2
AGTCCGC	20	7.033795E-4	45.0	27
TAGCGTC	20	7.033795E-4	45.0	32
AAGCGTC	20	7.033795E-4	45.0	45
ATCGTCC	20	7.033795E-4	45.0	32
CGCAGAT	20	7.033795E-4	45.0	28
CCGACAA	25	3.8912272E-5	45.0	25
GGCGCGT	20	7.033795E-4	45.0	8
TATACGT	20	7.033795E-4	45.0	22
TATCGCC	25	3.8912272E-5	45.0	1
ATACCGG	25	3.8912272E-5	45.0	2
TCAACGT	25	3.8912272E-5	45.0	40
GTCGGTG	25	3.8912272E-5	45.0	16
TAGTACG	25	3.8912272E-5	45.0	1
CGTTTTT	10235	0.0	44.120667	1
>>END_MODULE