FastQCFastQC Report
Thu 26 May 2016
SRR1548149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548149_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2809807
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144860.5155514239946017No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30850.10979401788094342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGAC253.892249E-545.0000049
CGCGATC453.8562575E-1045.044
CTACGCG207.0350297E-445.041
CGAATCG207.0350297E-445.015
CCGTATC302.1666274E-644.99999624
CGACGGT5150.041.9417528
CGTTTTT94900.041.277661
TCACGAC5250.041.14285325
TCGCGAT501.0822987E-940.50000443
CGGTCTA5350.039.9532731
CACGACG5450.039.6330326
CTCACGA5400.039.58333224
TTAGACG800.039.3751
CGTAAGG4250.039.1764682
GGGCGAT28850.038.916817
CTCGACC356.251512E-638.5714344
ACGGTCT5550.038.5135130
TACGGGA9950.038.216084
ACGGGAT9850.038.147215
TAGGGAC31400.037.8343965
Produced by FastQC (version 0.11.5)