FastQCFastQC Report
Thu 26 May 2016
SRR1548148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548148_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1369661
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65560.47865858778194015No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC17130.12506744369592185No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA207.0337614E-445.038
GTCGTTA253.891199E-545.010
CGAATCC207.0337614E-445.024
AAAGCGT207.0337614E-445.05
GCCGATT600.044.9999969
CGTTTTT37550.042.4833561
TAGGGCG3400.042.352945
CCGGATA700.041.78571720
TAATGCG700.041.7857171
CGGTCTA1100.040.90909231
TACGGGA6300.040.7142834
CTAGCGG1500.040.52
AGGGCGA9750.040.1538436
TTTCGCG403.458863E-739.3751
CGTTAGG2000.039.3752
TGTAGCG1200.039.3749961
CTACGGG3000.039.03
AATTCGC356.2491654E-638.5714313
CACGCCG1700.038.3823526
AAGGGAT26350.038.0834925
Produced by FastQC (version 0.11.5)