##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548148_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1369661
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137451529977124	31.0	31.0	33.0	30.0	34.0
2	31.522986344796266	31.0	31.0	34.0	30.0	34.0
3	31.641391556012763	31.0	31.0	34.0	30.0	34.0
4	35.43867497139803	37.0	35.0	37.0	33.0	37.0
5	35.291502057808465	37.0	35.0	37.0	33.0	37.0
6	35.337253524777296	37.0	35.0	37.0	33.0	37.0
7	35.76992190038265	37.0	35.0	37.0	35.0	37.0
8	35.77301463646844	37.0	35.0	37.0	35.0	37.0
9	37.49526050606683	39.0	37.0	39.0	35.0	39.0
10	36.78502198719245	39.0	37.0	39.0	32.0	39.0
11	36.44624180727932	39.0	35.0	39.0	32.0	39.0
12	35.88585788746266	37.0	35.0	39.0	31.0	39.0
13	35.634430709496726	37.0	35.0	39.0	30.0	39.0
14	36.57434576877052	38.0	35.0	41.0	31.0	41.0
15	36.83950116123625	38.0	35.0	41.0	31.0	41.0
16	36.93274394174909	38.0	35.0	41.0	32.0	41.0
17	36.805655560025436	38.0	35.0	41.0	31.0	41.0
18	36.74678186792206	38.0	35.0	40.0	31.0	41.0
19	36.70956389938824	38.0	35.0	40.0	31.0	41.0
20	36.59169531730844	38.0	35.0	40.0	31.0	41.0
21	36.450252288705016	38.0	35.0	40.0	31.0	41.0
22	36.37928290284968	38.0	35.0	40.0	30.0	41.0
23	36.327946842320834	38.0	35.0	40.0	30.0	41.0
24	36.196197453238426	38.0	34.0	40.0	30.0	41.0
25	36.09458909905444	38.0	34.0	40.0	30.0	41.0
26	36.0351050369398	38.0	34.0	40.0	30.0	41.0
27	36.0199224479634	38.0	34.0	40.0	30.0	41.0
28	36.054638337515634	38.0	35.0	40.0	30.0	41.0
29	36.087512165419035	38.0	35.0	40.0	30.0	41.0
30	36.034823215379575	38.0	35.0	40.0	30.0	41.0
31	35.904722409413715	38.0	34.0	40.0	30.0	41.0
32	35.780127345379626	38.0	34.0	40.0	29.0	41.0
33	35.680468378671804	38.0	34.0	40.0	29.0	41.0
34	35.57281108245033	38.0	34.0	40.0	28.0	41.0
35	35.42623685714933	38.0	34.0	40.0	27.0	41.0
36	35.28200554735807	38.0	34.0	40.0	27.0	41.0
37	35.2341155950268	38.0	34.0	40.0	27.0	41.0
38	35.172068854994045	38.0	34.0	40.0	27.0	41.0
39	35.1498903743335	38.0	34.0	40.0	27.0	41.0
40	35.041220418775154	37.0	34.0	40.0	26.0	41.0
41	35.00091482490923	37.0	34.0	40.0	26.0	41.0
42	34.9496254912712	37.0	34.0	40.0	26.0	41.0
43	34.86537617702483	37.0	33.0	40.0	26.0	41.0
44	34.75291112180313	37.0	33.0	40.0	25.0	41.0
45	34.681161980957334	37.0	33.0	40.0	25.0	41.0
46	34.58561936128721	37.0	33.0	40.0	24.0	41.0
47	34.5476690947614	37.0	33.0	40.0	24.0	41.0
48	34.451049566279536	37.0	33.0	40.0	24.0	41.0
49	34.35482867658494	36.0	33.0	40.0	24.0	41.0
50	34.22657139248325	36.0	33.0	40.0	24.0	41.0
51	33.85709383562794	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	5.0
13	3.0
14	19.0
15	60.0
16	138.0
17	323.0
18	617.0
19	1244.0
20	2233.0
21	3527.0
22	5346.0
23	7614.0
24	10830.0
25	15213.0
26	19956.0
27	24199.0
28	28054.0
29	32826.0
30	39742.0
31	48731.0
32	60157.0
33	76823.0
34	118706.0
35	151592.0
36	107607.0
37	134422.0
38	193431.0
39	286203.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.33493543292829	24.218547509201183	30.200246630370582	13.246270427499942
2	30.495283139404567	25.57421142895943	30.905822681670863	13.02468274996514
3	29.075880820144544	25.638752946897082	31.74508144716101	13.54028478579736
4	26.221378866741478	27.523014818995357	31.363162125518652	14.892444188744513
5	23.52049156689137	32.653992484271654	29.496714880543433	14.328801068293542
6	22.86872445079476	40.43664819250895	26.035493454219694	10.659133902476599
7	88.89871289319035	3.5730009104442635	5.911973838782005	1.6163123575833729
8	89.89786523818668	2.6829996619601495	5.682427987655339	1.736707112197836
9	84.92539394784548	5.712800466684822	7.064229761962997	2.2975758235066928
10	42.50248784188204	36.61416949157492	12.268291204903987	8.615051461639048
11	34.9265986254993	21.92257792256624	27.28857724648654	15.862246205447917
12	33.313352720125636	21.462902134177728	28.630515142068003	16.593230003628634
13	23.777270434070914	30.21557889141912	28.849766475062076	17.157384199447893
14	17.69744484219088	34.09062534451955	30.341887518152298	17.870042295137264
15	16.806640475270886	27.321505102357445	40.31092365191095	15.56093077046072
16	21.575922801335512	24.889735489292605	37.51300504285367	16.021336666518213
17	21.117926260585648	24.390560876012383	29.280237956691472	25.21127490671049
18	21.528830856686433	26.566427751100452	33.410311018565906	18.4944303736472
19	25.8001797525081	27.23951401113122	28.474272100906724	18.486034135453956
20	27.552292136521373	27.66589688981434	27.82119079100595	16.96062018265834
21	24.385669154630232	27.36837801470583	30.599177460700126	17.64677536996381
22	23.070818253567854	23.90401712540548	30.262086749933015	22.76307787109365
23	19.405020658396495	28.160617846313794	30.719937269149085	21.71442422614063
24	20.224055441455953	26.116243362408653	35.384522155482266	18.275179040653125
25	19.54578541697544	27.168766578007258	33.111916014254625	20.173531990762676
26	19.066688764592115	32.53396278349168	28.4549972584457	19.9443511934705
27	18.02037146417982	32.159563570839794	31.517871940575077	18.30219302440531
28	17.05582622269306	28.826841094256174	35.44147055366255	18.67586212938822
29	17.983501026896437	26.305049205606352	35.53930498130559	20.17214478619162
30	20.132353918232322	28.39615058032608	32.49979374458351	18.971701756858085
31	25.012466588447797	27.024132248782728	28.60021567380542	19.363185488964056
32	25.89750310478286	27.65005355339752	29.41910443533108	17.03333890648854
33	24.61944963023697	27.755919165399323	29.011631345274484	18.612999859089218
34	20.611815624450138	28.11586224620545	30.3453190241965	20.927003105147914
35	19.911204305298902	27.8063696053257	31.433617515575023	20.84880857380038
36	24.545927787970893	27.403496193583663	29.35062033598095	18.69995568246449
37	20.402712788054856	30.266321374413085	30.485207653572672	18.845758183959386
38	20.698333383224025	30.94532150656257	27.375387048328015	20.980958061885385
39	20.18243930432421	29.693405886566094	29.79934450933479	20.32481029977491
40	21.846646724992535	27.15956722137814	29.08172168149637	21.91206437213296
41	18.98542778103487	26.168300039206784	30.116722313039503	24.729549866718845
42	21.03045936184209	26.792396074649126	28.7000944029216	23.477050160587183
43	20.793539423258746	27.253313046074908	28.871596694364516	23.08155083630183
44	19.325292900944103	29.40618152959017	29.922367651557575	21.346157917908155
45	18.833784418188152	32.443429432538416	27.514691591569008	21.208094557704424
46	21.56015247568559	30.327139343238947	28.064024601708017	20.04868357936745
47	20.05656874219241	28.718347094646045	29.413993681648233	21.81109048151331
48	20.992201719987648	26.496702468713064	31.477716018781287	21.033379792518005
49	20.52339958573691	26.258541347092457	31.874821579938395	21.34323748723224
50	19.6066763965682	29.589730597571222	30.028525306626968	20.775067699233606
51	18.837800010367527	30.474110017004207	29.019370486565656	21.66871948606261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	635.0
1	1181.5
2	1728.0
3	6391.0
4	11054.0
5	7892.5
6	4731.0
7	4852.0
8	4973.0
9	5088.0
10	5203.0
11	5406.0
12	5609.0
13	5659.0
14	5709.0
15	5435.5
16	5162.0
17	4983.5
18	4805.0
19	4998.0
20	5191.0
21	5857.5
22	6524.0
23	7786.5
24	9049.0
25	10801.0
26	15977.5
27	19402.0
28	22591.5
29	25781.0
30	30127.0
31	34473.0
32	39375.0
33	44277.0
34	48917.5
35	53558.0
36	56299.0
37	59040.0
38	65710.5
39	72381.0
40	84351.0
41	96321.0
42	108120.5
43	119920.0
44	122304.5
45	124689.0
46	122051.0
47	119413.0
48	111248.5
49	103084.0
50	98427.0
51	93770.0
52	83282.5
53	72795.0
54	67276.0
55	61757.0
56	54952.0
57	48147.0
58	44611.0
59	41075.0
60	37592.5
61	34110.0
62	29821.5
63	25533.0
64	20460.0
65	15387.0
66	12047.5
67	8708.0
68	7000.0
69	5292.0
70	4272.0
71	3252.0
72	2736.0
73	2220.0
74	1645.5
75	860.5
76	650.0
77	460.5
78	271.0
79	253.5
80	236.0
81	139.0
82	42.0
83	34.5
84	27.0
85	23.5
86	20.0
87	14.0
88	8.0
89	7.5
90	7.0
91	6.5
92	6.0
93	8.5
94	11.0
95	6.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1369661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.291460797195104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2216221034098	17.8825714420556
2	6.160546412297114	2.7465515767804414
3	1.921222106649875	1.2848054181927087
4	1.0391848249226832	0.9265979114321621
5	0.6732305290537254	0.750364597293802
6	0.5371571253334173	0.7184410200782334
7	0.4303497467507647	0.6715187156144259
8	0.3643531769422213	0.6497571648112814
9	0.3475719195417723	0.6973097236804142
>10	5.993693188203155	34.22804156027779
>50	2.066220179721362	32.108677019025244
>100	0.24418960591657451	6.727927906178826
>500	0.0	0.0
>1k	3.295406287670891E-4	0.12583598658409986
>5k	3.295406287670891E-4	0.4815999579949555
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6556	0.47865858778194015	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1713	0.12506744369592185	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4602153379558884E-4	0.0	0.0	0.029642371360504533	0.0
2	1.4602153379558884E-4	0.0	0.0	0.1033102351603791	0.0
3	1.4602153379558884E-4	0.0	0.0	0.1543447612219374	0.0
4	1.4602153379558884E-4	0.0	0.0	0.2340725186743289	0.0
5	1.4602153379558884E-4	0.0	0.0	0.4388677198226422	0.0
6	1.4602153379558884E-4	0.0	0.0	0.6446850717075248	0.0
7	1.4602153379558884E-4	0.0	0.0	0.786618002556837	0.0
8	1.4602153379558884E-4	0.0	0.0	1.0934092450613693	0.0
9	1.4602153379558884E-4	0.0	0.0	1.1944561464479166	0.0
10	1.4602153379558884E-4	0.0	0.0	1.3966959707548072	0.0
11	1.4602153379558884E-4	0.0	0.0	1.6600458069551516	0.0
12	1.4602153379558884E-4	0.0	0.0	1.8830936998279135	0.0
13	1.4602153379558884E-4	0.0	0.0	1.971728770841836	0.0
14	1.4602153379558884E-4	0.0	0.0	2.0104974880645647	0.0
15	1.4602153379558884E-4	0.0	0.0	2.0707313707552455	0.0
16	1.4602153379558884E-4	0.0	0.0	2.2041950526444136	0.0
17	1.4602153379558884E-4	0.0	0.0	2.3684692781644507	0.0
18	1.4602153379558884E-4	0.0	0.0	2.566693510291963	0.0
19	1.4602153379558884E-4	0.0	0.0	2.6846789095987984	0.0
20	1.4602153379558884E-4	0.0	0.0	2.8228882913363234	0.0
21	1.4602153379558884E-4	0.0	0.0	2.9832199354438798	0.0
22	1.4602153379558884E-4	0.0	0.0	3.1630454543131474	0.0
23	1.4602153379558884E-4	0.0	0.0	3.345864414625225	0.0
24	1.4602153379558884E-4	0.0	0.0	3.4912288515187333	0.0
25	1.4602153379558884E-4	0.0	0.0	3.6189976935898738	0.0
26	1.4602153379558884E-4	0.0	0.0	3.7423858896471462	0.0
27	1.4602153379558884E-4	0.0	0.0	3.867818387177557	0.0
28	1.4602153379558884E-4	0.0	0.0	3.998945724525996	0.0
29	1.4602153379558884E-4	0.0	0.0	4.154458658018298	0.0
30	1.4602153379558884E-4	0.0	0.0	4.342315361246323	0.0
31	1.4602153379558884E-4	0.0	0.0	4.5111892650809216	0.0
32	1.4602153379558884E-4	0.0	0.0	4.658086928079284	0.0
33	1.4602153379558884E-4	0.0	0.0	4.81148254933155	0.0
34	1.4602153379558884E-4	0.0	0.0	4.974515591814325	0.0
35	1.4602153379558884E-4	0.0	0.0	5.171206597836983	0.0
36	1.4602153379558884E-4	0.0	0.0	5.347162546060668	0.0
37	1.4602153379558884E-4	0.0	0.0	5.530492581741029	0.0
38	1.4602153379558884E-4	0.0	0.0	5.72280294174982	0.0
39	2.1903230069338325E-4	0.0	0.0	5.981626110402501	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	20	7.0337614E-4	45.0	38
GTCGTTA	25	3.891199E-5	45.0	10
CGAATCC	20	7.0337614E-4	45.0	24
AAAGCGT	20	7.0337614E-4	45.0	5
GCCGATT	60	0.0	44.999996	9
CGTTTTT	3755	0.0	42.483356	1
TAGGGCG	340	0.0	42.35294	5
CCGGATA	70	0.0	41.785717	20
TAATGCG	70	0.0	41.785717	1
CGGTCTA	110	0.0	40.909092	31
TACGGGA	630	0.0	40.714283	4
CTAGCGG	150	0.0	40.5	2
AGGGCGA	975	0.0	40.153843	6
TTTCGCG	40	3.458863E-7	39.375	1
CGTTAGG	200	0.0	39.375	2
TGTAGCG	120	0.0	39.374996	1
CTACGGG	300	0.0	39.0	3
AATTCGC	35	6.2491654E-6	38.57143	13
CACGCCG	170	0.0	38.38235	26
AAGGGAT	2635	0.0	38.083492	5
>>END_MODULE