FastQCFastQC Report
Thu 26 May 2016
SRR1548147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548147_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2453378
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102370.4172614248599278No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36960.15064943111090098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCGA302.1665128E-645.00000416
CGTATCG207.034854E-445.013
CGTAAAC253.8921055E-545.035
TCCGTAC650.044.99999632
CGACGGT1950.042.69230728
CGTTTTT66550.040.604811
GCGTAAG2050.039.5121961
ATAGCGC356.25119E-638.57142611
ATCGTAC356.25119E-638.57142628
ATCCGGT356.25119E-638.57142629
CACGTTA356.25119E-638.57142617
TACACCG356.25119E-638.5714261
CGTAAGG4050.038.3333322
TTGCGAG2000.038.251
CGTATGG2600.038.076922
TTACGCG659.094947E-1238.076921
ACATACG2900.038.01724217
TATCGCG1250.037.81
ATAGGGC6550.037.786264
ACGGGAC6550.037.786265
Produced by FastQC (version 0.11.5)