FastQCFastQC Report
Thu 26 May 2016
SRR1548146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548146_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1992661
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469522.3562462455982227No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT28400.14252298810485076No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT20560.10317861392379335No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG600.044.9999961
CGTTTTT237600.043.9678041
CGACGGT2350.043.08510628
CTATGCG1000.042.751
TACGGGA9150.041.803284
CGGTCTA2500.041.39999831
GCGTTAG1450.040.344831
AGTACGG3250.040.1538472
CGAGGGA13600.038.7132384
AGGGCGA10900.038.6009186
GTTTTTT277750.038.308732
CACGACG2650.038.20754626
ACGGGAC7100.038.028175
AGGGATT40050.037.977536
TACGGGT1550.037.7419364
TCACGAC2750.037.6363625
GACCGAT7200.037.5000049
GTCGACA301.1402226E-437.49999635
CGACGAA301.1402226E-437.49999640
GCTATCG301.1402226E-437.49999620
Produced by FastQC (version 0.11.5)