##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1548146_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1992661 Sequences flagged as poor quality 0 Sequence length 51 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.255801664206807 31.0 31.0 33.0 30.0 34.0 2 31.63655333245344 31.0 31.0 34.0 30.0 34.0 3 31.503419798952255 31.0 31.0 34.0 30.0 34.0 4 35.43856983199852 37.0 35.0 37.0 33.0 37.0 5 35.37554456076573 37.0 35.0 37.0 33.0 37.0 6 35.4546433136394 37.0 35.0 37.0 33.0 37.0 7 35.853360406009855 37.0 35.0 37.0 35.0 37.0 8 35.864933874853776 37.0 35.0 37.0 35.0 37.0 9 37.60092660015928 39.0 37.0 39.0 35.0 39.0 10 37.011734058126294 39.0 37.0 39.0 33.0 39.0 11 36.74198722211154 39.0 35.0 39.0 32.0 39.0 12 36.340748376166346 38.0 35.0 39.0 32.0 39.0 13 36.199496050758256 38.0 35.0 39.0 32.0 39.0 14 37.234191365214656 39.0 35.0 41.0 32.0 41.0 15 37.41717482301305 39.0 35.0 41.0 32.0 41.0 16 37.47208632075401 39.0 35.0 41.0 32.0 41.0 17 37.345092316254494 39.0 35.0 41.0 32.0 41.0 18 37.122727849845006 39.0 35.0 40.0 32.0 41.0 19 36.918632421671326 38.0 35.0 40.0 32.0 41.0 20 36.66693531915364 38.0 35.0 40.0 31.0 41.0 21 36.53263500414772 38.0 35.0 40.0 31.0 41.0 22 36.46170372180717 38.0 35.0 40.0 31.0 41.0 23 36.39535977268587 38.0 35.0 40.0 31.0 41.0 24 36.224267449405595 38.0 35.0 40.0 31.0 41.0 25 36.097302049872006 37.0 35.0 40.0 31.0 41.0 26 36.025243129664304 37.0 35.0 40.0 30.0 41.0 27 35.95043913641106 37.0 35.0 40.0 30.0 41.0 28 35.90382508615364 37.0 35.0 40.0 30.0 41.0 29 35.8510208209023 37.0 35.0 40.0 30.0 41.0 30 35.69242585668109 37.0 35.0 40.0 30.0 41.0 31 35.45284571735985 37.0 34.0 40.0 29.0 41.0 32 35.08812637975049 37.0 34.0 40.0 26.0 41.0 33 34.73324062647887 37.0 34.0 40.0 24.0 41.0 34 34.323167864478705 37.0 33.0 40.0 22.0 41.0 35 33.94250251297135 37.0 33.0 40.0 20.0 41.0 36 33.72214792179904 37.0 33.0 40.0 18.0 41.0 37 33.59212128907024 37.0 33.0 40.0 18.0 41.0 38 33.48655039668062 36.0 33.0 40.0 18.0 41.0 39 33.419623307727704 36.0 33.0 40.0 18.0 41.0 40 33.32329131748953 36.0 33.0 40.0 18.0 41.0 41 33.22575189658452 36.0 33.0 40.0 16.0 41.0 42 33.17735329792674 36.0 32.0 40.0 16.0 41.0 43 33.0801300371714 36.0 32.0 40.0 15.0 41.0 44 32.934293389593115 36.0 32.0 40.0 15.0 41.0 45 32.853747325812066 35.0 32.0 40.0 15.0 41.0 46 32.756599341282836 35.0 31.0 40.0 15.0 41.0 47 32.71871030747327 35.0 31.0 40.0 15.0 41.0 48 32.5866472019074 35.0 31.0 40.0 15.0 41.0 49 32.47882805956457 35.0 31.0 39.0 15.0 41.0 50 32.34987637134465 35.0 31.0 39.0 14.0 41.0 51 32.01969476995836 35.0 30.0 39.0 13.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 0.0 10 1.0 11 4.0 12 11.0 13 8.0 14 29.0 15 86.0 16 222.0 17 560.0 18 1263.0 19 2416.0 20 4328.0 21 6908.0 22 10783.0 23 16367.0 24 24562.0 25 37027.0 26 51340.0 27 58001.0 28 56377.0 29 55329.0 30 59155.0 31 67495.0 32 81600.0 33 103135.0 34 154314.0 35 202714.0 36 172868.0 37 194108.0 38 267458.0 39 364145.0 40 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.32897266519493 22.338220098652002 25.848250153939883 22.48455708221318 2 38.21503005277867 23.5016894494347 26.552986182797778 11.730294314988852 3 26.258003744741327 23.58188372231905 38.13915161685806 12.020960916081561 4 23.735045750381023 25.387308729382468 37.8652465221129 13.012398998123615 5 21.548371750137125 29.017278904941683 36.42195034679757 13.012398998123615 6 20.39433701969377 36.61832092864768 32.815968195292626 10.171373856365935 7 79.69895531653403 3.0828625641792557 15.415216135609619 1.802965983677103 8 80.26834469084305 2.637227305597891 15.149691794038223 1.944736209520837 9 75.2076243776538 5.555586223647675 16.57090694302744 2.665882455671085 10 36.14192278566198 33.65449517002641 21.48087406739029 8.722707976921312 11 29.460655876739693 20.73262837983982 34.4210580725974 15.385657670823086 12 27.005095196824747 20.853020157467828 35.824959689580915 16.316924956126506 13 21.883601877087973 26.019679212871633 35.85757938756266 16.239139522477732 14 16.590930419173155 30.277252377599602 36.25604154444735 16.875775658779894 15 16.122310819552347 26.640607709991816 42.51882282033924 14.718258650116603 16 20.321921290174295 25.58864754215594 39.86960150271421 14.219829664955554 17 19.600775044024047 25.1159630263251 35.15490090888515 20.128361020765702 18 20.541226028913094 26.683163869820305 36.602613289465694 16.172996811800903 19 22.37129145399042 27.714598720003053 33.766907667686574 16.147202158319953 20 23.8278362451014 27.670486851501586 32.642280849577524 15.859396053819491 21 21.863076559434845 26.78142443697147 35.39944827544675 15.956050728146934 22 20.777041353245735 24.15724501056627 35.79831190553737 19.267401730650622 23 18.22462526240038 27.295912350369683 35.906258013781574 18.57320437344837 24 18.656058406322 26.17354381904398 38.338683800204855 16.831713974429167 25 18.547610456570386 27.95488043375165 35.60435016292284 17.893158946755115 26 17.721278230466698 30.975062993655218 33.37597313341306 17.927685642465025 27 17.307811012510406 29.54988329675745 36.2010898993858 16.941215791346345 28 16.428835612279258 28.55909760867503 38.23771328891367 16.77435349013204 29 17.458112543980135 26.969364081497055 37.90142929479725 17.671094079725552 30 18.851626041760237 27.155446912445218 36.6581169601854 17.334810085609142 31 22.525156060162768 26.046979390874814 33.44668260180733 17.981181947155086 32 22.846334624906092 27.60308953705623 33.18166010174335 16.368915736294333 33 21.971725245789425 27.804127244925255 32.29018884797765 17.93395866130767 34 19.710477597544187 28.745933201884316 33.28769921225938 18.25588998831211 35 19.83212397894072 29.419304136528996 32.94157912459771 17.806992759932573 36 23.069603911553447 29.245767343266117 31.102329999934756 16.58229874524568 37 20.524966364072966 31.398265936855292 30.865510992587296 17.211256706484445 38 20.01228508010143 31.901060943130823 29.397474030956595 18.689179945811155 39 20.04826711618283 31.180165617734275 30.255070982972015 18.516496283110875 40 21.522275991751734 29.155887529288727 30.10742921149157 19.214407267467973 41 18.466061211616026 28.926094303045026 31.142326768075453 21.4655177172635 42 20.455160210392034 29.570559166862804 29.369621827295262 20.604658795449904 43 20.319763371692424 29.50391461467856 29.688241000350786 20.488081013278226 44 19.63685744840693 31.13951645563395 30.41310087365588 18.81052522230324 45 18.905122346450298 32.40571276298377 28.968851199476482 19.72031369108945 46 20.632711735714203 31.092443722238755 29.451221256400363 18.823623285646683 47 19.815914498251335 30.334763414348952 30.09528464701221 19.7540374403875 48 20.339084269727767 28.749345724134713 31.400223118734193 19.511346887403327 49 19.940873033596784 27.79213323289812 32.0904559280279 20.1765378054772 50 18.849367754976885 30.26626204858729 31.523174288049997 19.361195908385824 51 18.18568236142525 31.053500821263626 30.953584177138012 19.807232640173115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1419.0 1 2267.0 2 3115.0 3 36854.5 4 70594.0 5 48477.0 6 26360.0 7 26490.0 8 26620.0 9 29038.5 10 31457.0 11 33218.5 12 34980.0 13 34673.5 14 34367.0 15 31897.5 16 29428.0 17 26425.5 18 23423.0 19 21441.5 20 19460.0 21 18258.5 22 17057.0 23 17617.5 24 18178.0 25 19220.0 26 22825.5 27 25389.0 28 28263.5 29 31138.0 30 36249.0 31 41360.0 32 45645.0 33 49930.0 34 55230.0 35 60530.0 36 66344.5 37 72159.0 38 78713.5 39 85268.0 40 95529.0 41 105790.0 42 118509.5 43 131229.0 44 133832.0 45 136435.0 46 133313.0 47 130191.0 48 134165.0 49 138139.0 50 135792.0 51 133445.0 52 124615.0 53 115785.0 54 103088.0 55 90391.0 56 81628.0 57 72865.0 58 66643.5 59 60422.0 60 54605.0 61 48788.0 62 42862.5 63 36937.0 64 31697.0 65 26457.0 66 21921.5 67 17386.0 68 13825.0 69 10264.0 70 8420.0 71 6576.0 72 5384.0 73 4192.0 74 3359.5 75 1878.0 76 1229.0 77 889.5 78 550.0 79 394.0 80 238.0 81 208.0 82 178.0 83 117.5 84 57.0 85 55.5 86 54.0 87 35.5 88 17.0 89 13.0 90 9.0 91 8.0 92 7.0 93 4.5 94 2.0 95 3.5 96 5.0 97 3.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1992661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.91532265126297 #Duplication Level Percentage of deduplicated Percentage of total 1 78.16575169529129 21.038564271572703 2 6.723258641604161 3.6191735121333592 3 2.7988082426315626 2.2599248066832835 4 1.7189870553311786 1.8506836491033236 5 1.151815815299068 1.5500747151800962 6 0.7647369240212891 1.2349884632020423 7 0.5962265563462829 1.1233341096118132 8 0.5009604671301958 1.078681008666931 9 0.42543212645378753 1.030557865474494 >10 5.917707395378873 38.71282775890834 >50 1.1572615335184728 20.416602743802784 >100 0.07584614153435053 2.8205120757414317 >500 0.0018867090937955528 0.3426219979058843 >1k 0.0011320254562773317 0.5371654800427516 >5k 0.0 0.0 >10k+ 1.8867090937955527E-4 2.3842875419708136 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46952 2.3562462455982227 No Hit CGTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT 2840 0.14252298810485076 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT 2056 0.10317861392379335 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.018415074114463E-5 0.0 0.0 0.04456352585813643 0.0 2 5.018415074114463E-5 0.0 0.0 0.14051562207520496 0.0 3 5.018415074114463E-5 0.0 0.0 0.28062977094448077 0.0 4 5.018415074114463E-5 0.0 0.0 0.37392210717226865 0.0 5 5.018415074114463E-5 0.0 0.0 0.6757295897295125 0.0 6 5.018415074114463E-5 0.0 0.0 1.2590701579445776 0.0 7 5.018415074114463E-5 0.0 0.0 1.6893490663991517 0.0 8 5.018415074114463E-5 0.0 0.0 2.3643760780182883 0.0 9 5.018415074114463E-5 0.0 0.0 2.814427541864873 0.0 10 5.018415074114463E-5 0.0 0.0 3.2167036942058886 0.0 11 5.018415074114463E-5 0.0 0.0 3.505162192665988 0.0 12 5.018415074114463E-5 0.0 0.0 3.7221584604706974 0.0 13 5.018415074114463E-5 0.0 0.0 3.845962760349101 0.0 14 5.018415074114463E-5 0.0 0.0 3.916722412894115 0.0 15 5.018415074114463E-5 0.0 0.0 3.9811086782950036 0.0 16 5.018415074114463E-5 0.0 0.0 4.062657923249364 0.0 17 5.018415074114463E-5 0.0 0.0 4.162725119827206 0.0 18 5.018415074114463E-5 0.0 0.0 4.279051981245179 0.0 19 5.018415074114463E-5 0.0 0.0 4.358844780923599 0.0 20 5.018415074114463E-5 0.0 0.0 4.443204338319464 0.0 21 5.018415074114463E-5 0.0 0.0 4.533134336447595 0.0 22 5.018415074114463E-5 0.0 0.0 4.63992620922475 0.0 23 5.018415074114463E-5 0.0 0.0 4.753292205748996 0.0 24 5.018415074114463E-5 0.0 0.0 4.841616311053411 0.0 25 5.018415074114463E-5 0.0 0.0 4.922864451103323 0.0 26 5.018415074114463E-5 0.0 0.0 4.999846938340239 0.0 27 5.018415074114463E-5 0.0 0.0 5.084005759133139 0.0 28 5.018415074114463E-5 0.0 0.0 5.172982258397188 0.0 29 5.018415074114463E-5 0.0 0.0 5.270339510835009 0.0 30 5.018415074114463E-5 0.0 0.0 5.410202738950579 0.0 31 5.018415074114463E-5 0.0 0.0 5.513531905326596 0.0 32 5.018415074114463E-5 0.0 0.0 5.621779118475245 0.0 33 5.018415074114463E-5 0.0 0.0 5.728470622950918 0.0 34 5.018415074114463E-5 0.0 0.0 5.834409365165475 0.0 35 5.018415074114463E-5 0.0 0.0 5.950987147337154 0.0 36 5.018415074114463E-5 0.0 0.0 6.063299276695836 0.0 37 5.018415074114463E-5 0.0 0.0 6.184845289790887 0.0 38 5.018415074114463E-5 0.0 0.0 6.3275188303479615 0.0 39 5.018415074114463E-5 0.0 0.0 6.509135271880164 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGATAG 60 0.0 44.999996 1 CGTTTTT 23760 0.0 43.967804 1 CGACGGT 235 0.0 43.085106 28 CTATGCG 100 0.0 42.75 1 TACGGGA 915 0.0 41.80328 4 CGGTCTA 250 0.0 41.399998 31 GCGTTAG 145 0.0 40.34483 1 AGTACGG 325 0.0 40.153847 2 CGAGGGA 1360 0.0 38.713238 4 AGGGCGA 1090 0.0 38.600918 6 GTTTTTT 27775 0.0 38.30873 2 CACGACG 265 0.0 38.207546 26 ACGGGAC 710 0.0 38.02817 5 AGGGATT 4005 0.0 37.97753 6 TACGGGT 155 0.0 37.741936 4 TCACGAC 275 0.0 37.63636 25 GACCGAT 720 0.0 37.500004 9 GTCGACA 30 1.1402226E-4 37.499996 35 CGACGAA 30 1.1402226E-4 37.499996 40 GCTATCG 30 1.1402226E-4 37.499996 20 >>END_MODULE