FastQCFastQC Report
Thu 26 May 2016
SRR1548145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548145_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences4130173
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT686961.6632717322010482No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT44150.10689624865592798TruSeq Adapter, Index 27 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT385700.043.821621
CGACGGT4200.041.78571328
TATCGCG1200.041.2499961
TACGGGA18750.040.4400024
CGTTAGG5500.038.8636362
ACGGGAT20800.038.6177865
TAGGGAC48700.038.162225
CGGTCTA4600.038.15217231
GTTTTTT460050.037.6540572
AGGGATC50850.037.610626
CGACGTA301.1404348E-437.49999629
GACCGAT13400.037.4440279
GGGCGAT40350.037.3605967
AAGGGAT72450.037.360255
GACGGTC4700.037.34042729
TTAGGGA57800.037.2923854
AGGGATT87100.037.2244536
CGGGATC12700.037.2047236
CACGACG4850.037.11340326
TAGGGAT67150.036.95835
Produced by FastQC (version 0.11.5)