##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548145_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	4130173
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.093163409862008	31.0	31.0	33.0	30.0	34.0
2	31.473011178950614	31.0	31.0	34.0	30.0	34.0
3	31.41708664503884	31.0	31.0	34.0	30.0	34.0
4	35.31756877980656	37.0	35.0	37.0	33.0	37.0
5	35.214005563447344	37.0	35.0	37.0	32.0	37.0
6	35.27175496038544	37.0	35.0	37.0	32.0	37.0
7	35.7370006050594	37.0	35.0	37.0	35.0	37.0
8	35.740162215965285	37.0	35.0	37.0	35.0	37.0
9	37.45237645009059	39.0	37.0	39.0	35.0	39.0
10	36.76572482557026	39.0	37.0	39.0	32.0	39.0
11	36.47679770314706	39.0	35.0	39.0	32.0	39.0
12	36.126336838674796	38.0	35.0	39.0	31.0	39.0
13	35.98706010619894	38.0	35.0	39.0	31.0	39.0
14	36.98867577701951	39.0	35.0	41.0	31.0	41.0
15	37.15489375384518	39.0	35.0	41.0	31.0	41.0
16	37.20048240110039	39.0	35.0	41.0	32.0	41.0
17	37.04902385444871	39.0	35.0	41.0	31.0	41.0
18	36.858991378811496	39.0	35.0	40.0	31.0	41.0
19	36.704621089721904	38.0	35.0	40.0	31.0	41.0
20	36.505115645276845	38.0	35.0	40.0	31.0	41.0
21	36.36500359670164	38.0	35.0	40.0	30.0	41.0
22	36.29865455999059	38.0	35.0	40.0	30.0	41.0
23	36.224143153325535	38.0	35.0	40.0	30.0	41.0
24	36.06702818501792	38.0	35.0	40.0	30.0	41.0
25	35.95155965621779	38.0	34.0	40.0	30.0	41.0
26	35.88816400668931	38.0	34.0	40.0	30.0	41.0
27	35.85304199121926	38.0	34.0	40.0	30.0	41.0
28	35.81373855284028	38.0	34.0	40.0	30.0	41.0
29	35.78838683028532	38.0	34.0	40.0	29.0	41.0
30	35.65076813973652	38.0	34.0	40.0	29.0	41.0
31	35.46816222952404	38.0	34.0	40.0	27.0	41.0
32	35.19524194264986	38.0	34.0	40.0	26.0	41.0
33	34.903930900715295	38.0	34.0	40.0	24.0	41.0
34	34.577062026215366	38.0	33.0	40.0	23.0	41.0
35	34.28487983433139	38.0	33.0	40.0	22.0	41.0
36	34.12175712736488	37.0	33.0	40.0	21.0	41.0
37	34.03399567039928	37.0	33.0	40.0	20.0	41.0
38	33.931611097162275	37.0	33.0	40.0	19.0	41.0
39	33.85566294680635	37.0	33.0	40.0	18.0	41.0
40	33.73300561501903	37.0	33.0	40.0	18.0	41.0
41	33.63492473559824	37.0	33.0	40.0	18.0	41.0
42	33.56059104545984	37.0	33.0	40.0	18.0	41.0
43	33.44125318721516	37.0	32.0	40.0	18.0	41.0
44	33.28786978172585	37.0	32.0	40.0	18.0	41.0
45	33.20634583587661	36.0	32.0	40.0	18.0	41.0
46	33.090523568867454	36.0	31.0	40.0	18.0	41.0
47	33.063990297742976	36.0	31.0	40.0	18.0	41.0
48	32.942226148880444	36.0	31.0	40.0	17.0	41.0
49	32.828985613919805	36.0	31.0	40.0	17.0	41.0
50	32.707085635395906	35.0	31.0	40.0	15.0	41.0
51	32.38125691103012	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	16.0
13	35.0
14	67.0
15	215.0
16	510.0
17	1378.0
18	3026.0
19	6093.0
20	10017.0
21	15646.0
22	23619.0
23	34090.0
24	49642.0
25	71598.0
26	98793.0
27	114198.0
28	116692.0
29	118829.0
30	129975.0
31	149546.0
32	177481.0
33	218645.0
34	311408.0
35	384639.0
36	331858.0
37	400678.0
38	564197.0
39	797149.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.171714356759388	23.574363592033553	27.016713343484643	19.237208707722413
2	35.988613552023125	23.782320982680385	27.728814265165163	12.500251200131327
3	26.870811464798205	23.924615264300066	36.37097041697769	12.833602853924036
4	24.273147880246178	25.902014273978352	35.8869713205718	13.937866525203665
5	22.136820903143768	29.941191325399686	34.31999095437407	13.601996817082481
6	20.432776060470108	38.1858822862868	31.260530733216257	10.120810920026836
7	82.18537092756164	3.1876630833623674	12.817477621397458	1.8094883676785454
8	82.8977381819115	2.5341069248188877	12.676006549846702	1.8921483434229027
9	78.064332898404	5.163149340233448	14.071783433768998	2.700734327593542
10	35.935177533725586	34.75132881842964	19.389187813682383	9.924305834162395
11	27.798520788354388	22.12180942541632	33.831270506102285	16.248399280127007
12	27.450811382477198	20.762762237804566	34.55593748736433	17.230488892353904
13	22.771903259258146	24.530764207697835	34.41308632834508	18.28424620469893
14	17.312640414820397	28.42704167597822	35.2241419427225	19.036175966478886
15	16.82864131841451	26.416544779117	40.43106668897404	16.323747213494446
16	21.147395036479104	25.289424922394293	37.95172744579949	15.61145259532712
17	21.063936062726672	24.70371095835453	33.949255878627845	20.283097100290956
18	21.287025991405205	25.784513142669812	35.72479893699368	17.203661928931307
19	22.78911803452301	26.935336606965375	32.86421658366368	17.41132877484793
20	24.605022598326993	26.968725038878517	32.18124277118658	16.245009591607907
21	23.11404389113967	25.807345116052037	34.73469513262519	16.3439158601831
22	21.847172019186605	22.847735433842605	35.52810015464243	19.77699239232836
23	19.45548043629165	25.776765283197577	35.12511461384305	19.642639666667716
24	19.354104537509688	25.05430159947295	37.300374584793424	18.291219278223938
25	19.41233454385567	27.021773664202442	34.77249015961317	18.793401632328717
26	18.795604929866133	29.20475728256419	32.99159623579933	19.00804155177035
27	17.991716085500535	28.373896202410894	35.04453687533186	18.589850836756717
28	17.016865879468	27.747384915837664	37.10200517024347	18.133744034450856
29	18.35138624943798	25.776281041980564	36.4049157262904	19.467416982291056
30	19.50150756396887	26.43032144174106	35.29757712328273	18.77059387100734
31	21.65822109630759	26.06232232887097	33.02706690494563	19.252389669875814
32	22.288654736738632	26.380444596388575	32.70468331471829	18.626217352154498
33	21.435639620906922	27.212831036375473	32.32697516544707	19.02455417727054
34	19.005935102476336	27.418996734519354	33.83647609918519	19.73859206381912
35	18.822867710384045	27.591168699228824	33.38339580448567	20.20256778590146
36	20.95701560200989	27.947400750525464	32.062240492105296	19.033343155359354
37	19.687020374207084	29.39947067592568	31.697970036606215	19.215538913261017
38	19.659660745445773	29.080597834521704	30.920908155663213	20.338833264369313
39	19.293768081869693	28.870921387554464	31.286728183056738	20.548582347519098
40	20.84329155219406	27.1934129635732	31.20443138822514	20.7588640960076
41	18.752459037430153	27.5063780621296	31.11017383533329	22.630989065106956
42	20.13247386973863	28.269445371900886	29.839645942191765	21.758434816168716
43	20.259490340961506	27.766221899179527	30.170987994933867	21.803299764925104
44	19.909650273729454	28.762984020281955	30.343934745590563	20.983430960398028
45	19.505744674617745	29.561909392173163	29.804853210749283	21.12749272245981
46	20.31055357729567	29.34862534813917	29.977775749345128	20.363045325220032
47	19.932191702381473	28.376559529104473	30.77125340754491	20.91999536096914
48	19.79483668117534	27.315320689956568	31.972437958409976	20.917404670458115
49	19.857279586109346	26.51663743867388	32.15565062286737	21.470432352349405
50	19.039831987667345	28.173299278262675	32.08868006255428	20.698188671515698
51	18.528497474560993	28.58238625839644	31.43870728901671	21.45040897802586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2692.0
1	4248.5
2	5805.0
3	58415.0
4	111025.0
5	78073.0
6	45121.0
7	44249.5
8	43378.0
9	44625.0
10	45872.0
11	45713.5
12	45555.0
13	44349.5
14	43144.0
15	40320.5
16	37497.0
17	35196.0
18	32895.0
19	30898.0
20	28901.0
21	29346.0
22	29791.0
23	30211.0
24	30631.0
25	35541.0
26	47175.0
27	53899.0
28	61004.5
29	68110.0
30	78282.5
31	88455.0
32	101405.0
33	114355.0
34	126516.0
35	138677.0
36	150873.5
37	163070.0
38	176026.5
39	188983.0
40	213680.0
41	238377.0
42	259475.5
43	280574.0
44	296894.0
45	313214.0
46	317517.0
47	321820.0
48	314159.0
49	306498.0
50	295110.5
51	283723.0
52	259066.0
53	234409.0
54	211177.5
55	187946.0
56	173066.5
57	158187.0
58	147027.5
59	135868.0
60	122698.0
61	109528.0
62	94371.5
63	79215.0
64	65836.5
65	52458.0
66	41513.5
67	30569.0
68	23992.5
69	17416.0
70	14056.0
71	10696.0
72	7832.0
73	4968.0
74	4229.0
75	2428.0
76	1366.0
77	1020.5
78	675.0
79	586.5
80	498.0
81	346.5
82	195.0
83	148.5
84	102.0
85	63.5
86	25.0
87	22.5
88	20.0
89	17.5
90	15.0
91	9.5
92	4.0
93	4.5
94	5.0
95	3.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4130173.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.59719524313824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.66882996043906	20.08824155815577
2	6.054400608746867	2.978425477070434
3	2.128706320367401	1.5708061493213799
4	1.2003209073011394	1.1809811084522783
5	0.797816831703221	0.9812028188833043
6	0.5959845097639932	0.8795728409130581
7	0.45732331123261827	0.787420954293903
8	0.3631642356636449	0.7146257287954992
9	0.3237705092806102	0.7167461787670922
>10	4.389953626768709	27.02080143411014
>50	1.6250659755808305	28.19086485845824
>100	0.3909680812468471	12.17850809272156
>500	0.002396835830321876	0.41850992177822777
>1k	0.001198417915152175	0.605791699296686
>5k	0.0	0.0
>10k+	9.986815959601459E-5	1.6875011789824073
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	68696	1.6632717322010482	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTT	4415	0.10689624865592798	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.684824340287925E-5	0.0	0.0	0.045736582947009725	0.0
2	9.684824340287925E-5	0.0	0.0	0.1472819661549286	0.0
3	9.684824340287925E-5	0.0	0.0	0.2706424161893461	0.0
4	9.684824340287925E-5	0.0	0.0	0.38330113532774535	0.0
5	9.684824340287925E-5	0.0	0.0	0.6861940165702503	0.0
6	9.684824340287925E-5	0.0	0.0	1.2221764076226347	0.0
7	9.684824340287925E-5	0.0	0.0	1.5786263674669319	0.0
8	9.684824340287925E-5	0.0	0.0	2.1370049148062322	0.0
9	9.684824340287925E-5	0.0	0.0	2.4518827661698435	0.0
10	9.684824340287925E-5	0.0	0.0	2.800439594176806	0.0
11	9.684824340287925E-5	0.0	0.0	3.0870861825884774	0.0
12	9.684824340287925E-5	0.0	0.0	3.30782754136449	0.0
13	9.684824340287925E-5	0.0	0.0	3.4192030212778013	0.0
14	9.684824340287925E-5	0.0	0.0	3.4875052449376818	0.0
15	9.684824340287925E-5	0.0	0.0	3.55471792585928	0.0
16	9.684824340287925E-5	0.0	0.0	3.6623163242798786	0.0
17	9.684824340287925E-5	0.0	0.0	3.79487735743757	0.0
18	9.684824340287925E-5	0.0	0.0	3.957243437502497	0.0
19	9.684824340287925E-5	0.0	0.0	4.05588337340833	0.0
20	9.684824340287925E-5	0.0	0.0	4.169195818189698	0.0
21	9.684824340287925E-5	0.0	0.0	4.302483213172911	0.0
22	9.684824340287925E-5	0.0	0.0	4.455237105080102	0.0
23	9.684824340287925E-5	0.0	0.0	4.617530548962477	0.0
24	9.684824340287925E-5	0.0	0.0	4.747355619244036	0.0
25	0.0017674804421025463	0.0	0.0	4.858416342366288	0.0
26	0.0017674804421025463	0.0	0.0	4.9695981257927935	0.0
27	0.0017674804421025463	0.0	0.0	5.0852591404766825	0.0
28	0.0017674804421025463	0.0	0.0	5.208982771423861	0.0
29	0.0017674804421025463	0.0	0.0	5.342197530224521	0.0
30	0.0017674804421025463	0.0	0.0	5.511560895875306	0.0
31	0.0017674804421025463	0.0	0.0	5.662305186731888	0.0
32	0.0017674804421025463	0.0	0.0	5.808400761905131	0.0
33	0.0017674804421025463	0.0	0.0	5.95268043251457	0.0
34	0.0017674804421025463	0.0	0.0	6.108242923480445	0.0
35	0.0017674804421025463	0.0	0.0	6.271383789492595	0.0
36	0.0017674804421025463	0.0	0.0	6.433047719792851	0.0
37	0.0017674804421025463	0.0	0.0	6.60219317689598	0.0
38	0.0017674804421025463	0.0	0.0	6.7956475430932315	0.0
39	0.0017674804421025463	0.0	0.0	7.027478025738874	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	38570	0.0	43.82162	1
CGACGGT	420	0.0	41.785713	28
TATCGCG	120	0.0	41.249996	1
TACGGGA	1875	0.0	40.440002	4
CGTTAGG	550	0.0	38.863636	2
ACGGGAT	2080	0.0	38.617786	5
TAGGGAC	4870	0.0	38.16222	5
CGGTCTA	460	0.0	38.152172	31
GTTTTTT	46005	0.0	37.654057	2
AGGGATC	5085	0.0	37.61062	6
CGACGTA	30	1.1404348E-4	37.499996	29
GACCGAT	1340	0.0	37.444027	9
GGGCGAT	4035	0.0	37.360596	7
AAGGGAT	7245	0.0	37.36025	5
GACGGTC	470	0.0	37.340427	29
TTAGGGA	5780	0.0	37.292385	4
AGGGATT	8710	0.0	37.224453	6
CGGGATC	1270	0.0	37.204723	6
CACGACG	485	0.0	37.113403	26
TAGGGAT	6715	0.0	36.9583	5
>>END_MODULE