FastQCFastQC Report
Thu 26 May 2016
SRR1548144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548144_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1783756
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100550.5636981739654976No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCTA207.034334E-445.00000419
ACTCGAT207.034334E-445.00000440
CAACGCG502.1827873E-1145.01
TAATACG302.166178E-644.9999961
TAACGCG302.166178E-644.9999961
TATAGCG850.042.352941
TCGGACG603.6379788E-1241.2499961
CGTTTTT71700.041.234311
GCGATAT950.040.2631579
CGGTCTA3000.039.00000431
GACCGAT5200.038.942319
AGGGCGA11000.038.8636366
GGCGATA5000.038.78
ATCGGTT356.2502277E-638.5714328
TAAGGGA24850.038.480894
TATACGG2650.038.2075462
GGGCGAT21300.038.13387
ACGAGGG6750.037.6666683
CGGGATA4600.037.663046
CGAACCG301.14017465E-437.49999619
Produced by FastQC (version 0.11.5)