##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548144_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1783756
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.141477870291677	31.0	31.0	33.0	30.0	34.0
2	31.527748189774833	31.0	31.0	34.0	30.0	34.0
3	31.63210383034451	31.0	31.0	34.0	30.0	34.0
4	35.44766997279897	37.0	35.0	37.0	33.0	37.0
5	35.29979548772366	37.0	35.0	37.0	33.0	37.0
6	35.341326392174714	37.0	35.0	37.0	33.0	37.0
7	35.77397917652414	37.0	35.0	37.0	35.0	37.0
8	35.775084708895164	37.0	35.0	37.0	35.0	37.0
9	37.510880972509696	39.0	37.0	39.0	35.0	39.0
10	36.78179470734786	39.0	37.0	39.0	32.0	39.0
11	36.47888051953294	39.0	35.0	39.0	32.0	39.0
12	35.95716622677092	37.0	35.0	39.0	31.0	39.0
13	35.74854128030964	37.0	35.0	39.0	30.0	39.0
14	36.71209066710918	39.0	35.0	41.0	31.0	41.0
15	36.954873312269164	39.0	35.0	41.0	31.0	41.0
16	37.01710603916679	39.0	35.0	41.0	32.0	41.0
17	36.881349242833664	38.0	35.0	41.0	31.0	41.0
18	36.77834692637334	38.0	35.0	40.0	31.0	41.0
19	36.72074712012181	38.0	35.0	40.0	31.0	41.0
20	36.61729126629427	38.0	35.0	40.0	31.0	41.0
21	36.47956166650596	38.0	35.0	40.0	31.0	41.0
22	36.400833409950685	38.0	35.0	40.0	30.0	41.0
23	36.33096006404463	38.0	35.0	40.0	30.0	41.0
24	36.17895777225136	38.0	35.0	40.0	30.0	41.0
25	36.07791592572078	38.0	34.0	40.0	30.0	41.0
26	36.014537862801866	38.0	34.0	40.0	30.0	41.0
27	36.00854320882453	38.0	34.0	40.0	30.0	41.0
28	36.016685578072334	38.0	35.0	40.0	30.0	41.0
29	36.046346585519544	38.0	35.0	40.0	30.0	41.0
30	35.960915618503876	38.0	35.0	40.0	30.0	41.0
31	35.83890397565586	38.0	34.0	40.0	29.0	41.0
32	35.685046609513854	38.0	34.0	40.0	29.0	41.0
33	35.55371642758315	38.0	34.0	40.0	28.0	41.0
34	35.40701586988355	38.0	34.0	40.0	27.0	41.0
35	35.248448778868855	38.0	34.0	40.0	26.0	41.0
36	35.10106483173708	38.0	34.0	40.0	25.0	41.0
37	35.02824825816984	38.0	34.0	40.0	25.0	41.0
38	34.942923247349974	38.0	34.0	40.0	25.0	41.0
39	34.91392040166929	38.0	34.0	40.0	25.0	41.0
40	34.80660359376507	37.0	34.0	40.0	24.0	41.0
41	34.75560782977044	37.0	34.0	40.0	24.0	41.0
42	34.705801690365725	37.0	33.0	40.0	24.0	41.0
43	34.60784434642406	37.0	33.0	40.0	24.0	41.0
44	34.47797064172454	37.0	33.0	40.0	24.0	41.0
45	34.39809704914798	37.0	33.0	40.0	23.0	41.0
46	34.284264215509296	37.0	33.0	40.0	23.0	41.0
47	34.26028728144432	37.0	33.0	40.0	23.0	41.0
48	34.141659509484484	36.0	33.0	40.0	23.0	41.0
49	34.038674572082726	36.0	33.0	40.0	23.0	41.0
50	33.913670928086574	36.0	33.0	40.0	23.0	41.0
51	33.55674711115197	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	5.0
12	2.0
13	9.0
14	23.0
15	60.0
16	206.0
17	439.0
18	979.0
19	1938.0
20	3223.0
21	5172.0
22	7864.0
23	11184.0
24	15771.0
25	22386.0
26	29446.0
27	34875.0
28	39052.0
29	44215.0
30	51843.0
31	62924.0
32	77046.0
33	97021.0
34	147685.0
35	188057.0
36	140104.0
37	175146.0
38	253501.0
39	373522.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.761431496236035	23.351456140862314	29.899885410336392	13.98722695256526
2	30.96735203693779	25.608659480332513	30.230423892056983	13.193564590672716
3	28.6837998022151	25.37891953832251	32.830218931288805	13.107061728173584
4	25.5114488752946	27.502192003839088	32.29511211174623	14.691247009120081
5	23.92591811884585	31.999499931604998	30.39143246049348	13.68314948905568
6	21.90361237747764	41.099287122229725	27.000778133332137	9.996322366960504
7	88.47224620407724	3.434214096546837	6.6816313441973	1.4119083551786231
8	89.92244454959086	2.5340909855383806	6.258423237258907	1.2850412276118484
9	85.1126499364263	5.435048291358235	7.498222851107439	1.954078921108044
10	40.07067110075593	36.28265300859535	13.874319133334378	9.772356757314341
11	34.19228863140474	22.416687035670797	27.561448987417563	15.829575345506896
12	32.10052271723263	22.035468976698606	29.10493363442085	16.759074671647916
13	24.422062210302308	29.076118034080896	28.880463471461344	17.621356284155457
14	18.489524351985363	33.00894292717165	30.60665247937498	17.894880241468
15	18.468164928387065	27.508751196912584	38.531054695821624	15.492029178878724
16	22.673728918080723	25.428926377822975	35.85294177006272	16.044402934033577
17	22.866019791944638	24.10682851241986	29.38950170314774	23.63764999248776
18	23.38840065569506	26.765151735999765	32.018841141949906	17.827606466355263
19	25.82606589690518	27.418099784948165	28.80119253978683	17.95464177835982
20	27.420005875243024	27.36119738349864	27.836991157983494	17.381805583274843
21	24.953020480379603	27.171821706556276	30.813519337846657	17.061638475217464
22	23.807292028730387	23.83509852244365	30.714066273638323	21.643543175187638
23	20.75732331103581	26.836125568743707	30.99958738751264	21.406963732707837
24	20.821513704789222	25.783122803791546	35.10356797678607	18.291795514633165
25	20.231578758529754	27.701154193735018	32.64235691428649	19.424910133448744
26	20.0720838500333	31.8997104985211	28.23362612375235	19.794579527693248
27	18.862669557944024	31.166202103875197	31.758996185576947	18.212132152603832
28	17.239409425952875	29.379018206526002	34.92703037859438	18.45454198892674
29	18.719152171036846	26.808431197988963	34.79343587351633	19.678980757457857
30	20.36220200520699	28.73201267437923	32.973287826361904	17.932497494051876
31	25.194925763389165	26.899138671432638	29.312753538039953	18.59318202713824
32	24.88742854964468	27.47696433817181	29.802730866777743	17.832876245405764
33	23.739065208470215	28.24158685380736	29.067316381836978	18.952031555885444
34	20.491704022299015	28.653246296018064	30.4520349195742	20.403014762108718
35	20.055321467734377	28.543926411459864	31.46966289111291	19.93108922969285
36	24.736398924516582	28.387627007281264	29.123994537369462	17.751979530832692
37	21.275051072007606	30.664451864492676	29.60029286516766	18.460204198332058
38	21.049123310587323	31.377385696249938	26.853000074001155	20.720490919161588
39	21.01980315693402	29.96979407497438	28.94560691036218	20.06479585772942
40	21.925083924034453	28.562314576657343	28.18933755513646	21.323263944171735
41	19.69860227519907	27.35273210013029	29.562899858500824	23.385765766169815
42	21.84093564366427	27.608708814434262	27.58168718143064	22.968668360470826
43	21.606878967751193	27.617398343719657	28.2724206674007	22.503302021128448
44	19.70258264022658	29.85974539118579	29.26672706356699	21.17094490502064
45	20.0504441190387	31.7891011999399	27.64380330045141	20.51665138056999
46	22.03692657515938	30.321467734376228	28.0926315034119	19.54897418705249
47	20.717743906677818	29.18734400893396	28.63911880324439	21.455793281143833
48	21.33178528901935	27.903872502741407	30.46823668708052	20.296105521158722
49	21.12071381960313	26.72013436815349	31.021619548862063	21.137532263381313
50	20.20685564617582	30.005449175784133	29.43435088655623	20.353344291483815
51	19.399121852988863	30.744956148710923	28.257452252438114	21.598469745862104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	775.0
1	1517.5
2	2260.0
3	10210.5
4	18161.0
5	13348.5
6	8536.0
7	8532.0
8	8528.0
9	8913.0
10	9298.0
11	9555.5
12	9813.0
13	9518.5
14	9224.0
15	8739.0
16	8254.0
17	7714.0
18	7174.0
19	7605.5
20	8037.0
21	8926.0
22	9815.0
23	10665.5
24	11516.0
25	13937.0
26	19114.0
27	21870.0
28	24943.5
29	28017.0
30	33814.5
31	39612.0
32	46982.0
33	54352.0
34	59738.5
35	65125.0
36	69951.0
37	74777.0
38	84695.5
39	94614.0
40	108839.5
41	123065.0
42	136826.5
43	150588.0
44	152118.0
45	153648.0
46	149691.5
47	145735.0
48	141296.5
49	136858.0
50	130518.5
51	124179.0
52	113507.0
53	102835.0
54	92811.0
55	82787.0
56	74446.0
57	66105.0
58	61771.0
59	57437.0
60	50711.5
61	43986.0
62	38785.0
63	33584.0
64	28523.0
65	23462.0
66	18331.0
67	13200.0
68	11276.0
69	9352.0
70	7178.5
71	5005.0
72	3951.0
73	2897.0
74	2205.5
75	1101.0
76	688.0
77	577.0
78	466.0
79	289.0
80	112.0
81	84.5
82	57.0
83	42.0
84	27.0
85	20.5
86	14.0
87	18.5
88	23.0
89	16.0
90	9.0
91	6.0
92	3.0
93	3.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1783756.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.571589515883726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16053571405443	17.507617613128033
2	5.797440598333599	2.501200176599435
3	1.7732801366375346	1.1475741361264533
4	0.9768423667197321	0.8428817022640969
5	0.6953807992521542	0.7500234579347309
6	0.49630994915336585	0.6423716697471321
7	0.41892162145651163	0.632576368017181
8	0.3791957702004255	0.6543884400738363
9	0.33182378865931794	0.6442169904507744
>10	5.467903336937123	29.93704194701494
>50	1.997778827426827	30.22289446860398
>100	0.5032832485582048	13.777219085698441
>500	7.823055665393293E-4	0.08702171280087549
>1k	2.6076852217977645E-4	0.08735922429202303
>5k	0.0	0.0
>10k+	2.6076852217977645E-4	0.565613007248095
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10055	0.5636981739654976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6061479260616365E-5	0.0	0.0	0.01788361188413662	0.0
2	5.6061479260616365E-5	0.0	0.0	0.05701452440804684	0.0
3	5.6061479260616365E-5	0.0	0.0	0.09143627267406529	0.0
4	5.6061479260616365E-5	0.0	0.0	0.1305671851979755	0.0
5	5.6061479260616365E-5	0.0	0.0	0.23624307360423735	0.0
6	5.6061479260616365E-5	0.0	0.0	0.3586813443094235	0.0
7	5.6061479260616365E-5	0.0	0.0	0.4433341779929542	0.0
8	5.6061479260616365E-5	0.0	0.0	0.630691641681934	0.0
9	5.6061479260616365E-5	0.0	0.0	0.7040200565548203	0.0
10	5.6061479260616365E-5	0.0	0.0	0.829149278264516	0.0
11	5.6061479260616365E-5	0.0	0.0	0.9782728130977555	0.0
12	5.6061479260616365E-5	0.0	0.0	1.095833735107268	0.0
13	5.6061479260616365E-5	0.0	0.0	1.1484754641329868	0.0
14	5.6061479260616365E-5	0.0	0.0	1.1730864535283974	0.0
15	5.6061479260616365E-5	0.0	0.0	1.205882418895858	0.0
16	5.6061479260616365E-5	0.0	0.0	1.2744456080315918	0.0
17	5.6061479260616365E-5	0.0	0.0	1.370871352359852	0.0
18	5.6061479260616365E-5	0.0	0.0	1.4831624953188665	0.0
19	5.6061479260616365E-5	0.0	0.0	1.549595348242697	0.0
20	5.6061479260616365E-5	0.0	0.0	1.6223070868437164	0.0
21	5.6061479260616365E-5	0.0	0.0	1.7175555401075036	0.0
22	5.6061479260616365E-5	0.0	0.0	1.8258663180390142	0.0
23	5.6061479260616365E-5	0.0	0.0	1.945277268864127	0.0
24	5.6061479260616365E-5	0.0	0.0	2.0289770574002275	0.0
25	5.6061479260616365E-5	0.0	0.0	2.1088086038673453	0.0
26	5.6061479260616365E-5	0.0	0.0	2.1871825518736867	0.0
27	5.6061479260616365E-5	0.0	0.0	2.271330832243872	0.0
28	5.6061479260616365E-5	0.0	0.0	2.3577776332637423	0.0
29	5.6061479260616365E-5	0.0	0.0	2.453250332444572	0.0
30	5.6061479260616365E-5	0.0	0.0	2.5718764225600363	0.0
31	5.6061479260616365E-5	0.0	0.0	2.6800750775330258	0.0
32	5.6061479260616365E-5	0.0	0.0	2.7811539246399173	0.0
33	5.6061479260616365E-5	0.0	0.0	2.887278304880264	0.0
34	5.6061479260616365E-5	0.0	0.0	3.007249870497983	0.0
35	5.6061479260616365E-5	0.0	0.0	3.1408443755760316	0.0
36	5.6061479260616365E-5	0.0	0.0	3.260535633797448	0.0
37	5.6061479260616365E-5	0.0	0.0	3.3882436835531315	0.0
38	5.6061479260616365E-5	0.0	0.0	3.530695902354358	0.0
39	5.6061479260616365E-5	0.0	0.0	3.7113259885320637	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCTA	20	7.034334E-4	45.000004	19
ACTCGAT	20	7.034334E-4	45.000004	40
CAACGCG	50	2.1827873E-11	45.0	1
TAATACG	30	2.166178E-6	44.999996	1
TAACGCG	30	2.166178E-6	44.999996	1
TATAGCG	85	0.0	42.35294	1
TCGGACG	60	3.6379788E-12	41.249996	1
CGTTTTT	7170	0.0	41.23431	1
GCGATAT	95	0.0	40.263157	9
CGGTCTA	300	0.0	39.000004	31
GACCGAT	520	0.0	38.94231	9
AGGGCGA	1100	0.0	38.863636	6
GGCGATA	500	0.0	38.7	8
ATCGGTT	35	6.2502277E-6	38.57143	28
TAAGGGA	2485	0.0	38.48089	4
TATACGG	265	0.0	38.207546	2
GGGCGAT	2130	0.0	38.1338	7
ACGAGGG	675	0.0	37.666668	3
CGGGATA	460	0.0	37.66304	6
CGAACCG	30	1.14017465E-4	37.499996	19
>>END_MODULE