FastQCFastQC Report
Thu 26 May 2016
SRR1548143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548143_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2167086
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200770.9264514652394967No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC22340.10308774086492184No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG900.045.01
TATACGC207.034671E-445.044
CGATACT351.2125929E-745.010
TCGCGAT253.8919545E-544.99999635
CGTTTTT120100.042.6019971
CGACGGT2800.042.58928328
TACGGGT1400.041.7857134
TCACGAC2950.041.1864425
CACGACG2900.041.1206926
CGGTCTA3000.040.531
GTTAGCG1300.039.807691
TAGGGAC24450.039.5705535
TACGGGA9500.038.6052634
CGTAAGG2300.038.1521722
ACGGGTC1300.038.076925
AGTAGCG1300.038.076921
GACGGTC3150.037.85714329
GCGTAAG1250.037.8000031
GTCGATC301.1402555E-437.49999639
CGCGACC601.5643309E-1037.49999610
Produced by FastQC (version 0.11.5)