FastQCFastQC Report
Thu 26 May 2016
SRR1548142_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548142_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2558855
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196420.7676089500968206No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC34870.1362718872308122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG302.1665492E-645.0000041
TAACGAC253.8921513E-545.028
TACGACG800.045.01
GTACGTA207.034911E-445.031
TAATCCG207.034911E-445.01
CGTTTTT128050.043.313161
CGACGGT3550.043.0985928
TTAGACG1550.040.645161
CACGACG3800.040.26315726
TCCGATT403.4604454E-739.37529
TCGTGCG403.4604454E-739.3751
CGGTCTA4000.039.37531
GTTGCGA403.4604454E-739.37537
TCACGAC3950.039.303825
TCGATAG750.039.01
AGTACGG2950.038.8983042
GTACGGG10100.038.762383
CGGCGAA356.2512954E-638.57142611
TAGTACG1400.038.5714261
TAACGGG5050.038.3168333
Produced by FastQC (version 0.11.5)