##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548142_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2558855
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081072589107237	31.0	31.0	33.0	30.0	34.0
2	31.455925013336042	31.0	31.0	34.0	30.0	34.0
3	31.505986075803435	31.0	31.0	34.0	30.0	34.0
4	35.348828284525695	37.0	35.0	37.0	33.0	37.0
5	35.20784686900977	37.0	35.0	37.0	32.0	37.0
6	35.25960791056938	37.0	35.0	37.0	32.0	37.0
7	35.74243323674065	37.0	35.0	37.0	35.0	37.0
8	35.74157464959914	37.0	35.0	37.0	35.0	37.0
9	37.475735436357276	39.0	37.0	39.0	35.0	39.0
10	36.67310144576383	39.0	37.0	39.0	32.0	39.0
11	36.374903228201674	38.0	35.0	39.0	32.0	39.0
12	35.77998245308937	37.0	35.0	39.0	31.0	39.0
13	35.51737007372438	37.0	35.0	39.0	30.0	39.0
14	36.45269075426314	38.0	35.0	40.0	30.0	41.0
15	36.73936076878135	38.0	35.0	41.0	31.0	41.0
16	36.83912218550875	38.0	35.0	41.0	31.0	41.0
17	36.721442989149445	38.0	35.0	40.0	31.0	41.0
18	36.61096115254674	38.0	35.0	40.0	31.0	41.0
19	36.519496415388915	38.0	35.0	40.0	31.0	41.0
20	36.371292238129946	38.0	35.0	40.0	31.0	41.0
21	36.21822768386642	38.0	34.0	40.0	30.0	41.0
22	36.138981302183986	38.0	34.0	40.0	30.0	41.0
23	36.084564385242615	38.0	34.0	40.0	30.0	41.0
24	35.93612455570949	37.0	34.0	40.0	30.0	41.0
25	35.81885022793398	37.0	34.0	40.0	30.0	41.0
26	35.76207600665141	37.0	34.0	40.0	30.0	41.0
27	35.73344445074066	37.0	34.0	40.0	30.0	41.0
28	35.75137825316401	37.0	34.0	40.0	30.0	41.0
29	35.784277342795896	37.0	34.0	40.0	30.0	41.0
30	35.689902710391955	37.0	34.0	40.0	29.0	41.0
31	35.52549949098327	37.0	34.0	40.0	29.0	41.0
32	35.335431276879696	37.0	34.0	40.0	28.0	41.0
33	35.17219107764996	37.0	34.0	40.0	27.0	41.0
34	34.995438584835796	37.0	34.0	40.0	26.0	41.0
35	34.82715863149729	37.0	34.0	40.0	25.0	41.0
36	34.62671311973519	37.0	33.0	40.0	24.0	41.0
37	34.53435188785609	37.0	33.0	40.0	24.0	41.0
38	34.45024122117119	37.0	33.0	40.0	24.0	41.0
39	34.428350180061	37.0	33.0	40.0	23.0	41.0
40	34.30363072546119	37.0	33.0	40.0	23.0	41.0
41	34.26458279191279	37.0	33.0	40.0	23.0	41.0
42	34.20708402781713	37.0	33.0	40.0	23.0	41.0
43	34.11986572119171	36.0	33.0	40.0	23.0	41.0
44	33.99301054573237	36.0	33.0	40.0	23.0	41.0
45	33.91010080680617	36.0	33.0	40.0	23.0	41.0
46	33.80121147935307	36.0	33.0	40.0	23.0	41.0
47	33.76049834789388	36.0	33.0	40.0	23.0	41.0
48	33.652222185313356	36.0	32.0	40.0	23.0	41.0
49	33.551479470309964	36.0	32.0	40.0	22.0	41.0
50	33.40534496874579	35.0	32.0	40.0	21.0	41.0
51	33.058301076067224	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	11.0
13	11.0
14	37.0
15	98.0
16	286.0
17	699.0
18	1518.0
19	2997.0
20	5052.0
21	8201.0
22	12533.0
23	18410.0
24	26110.0
25	36947.0
26	48375.0
27	55544.0
28	59807.0
29	66979.0
30	79523.0
31	96029.0
32	116972.0
33	148694.0
34	226492.0
35	294584.0
36	197702.0
37	242173.0
38	339908.0
39	473091.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.695973394350208	24.300009183795098	29.489674092514033	14.514343329340662
2	31.638369505110685	25.250707836122015	29.860855734303037	13.250066924464262
3	27.78058936516528	24.885974390889675	33.88390510599467	13.449531137950371
4	25.280682180115715	27.079729019424704	33.023403045502775	14.616185754956806
5	22.99559764035086	31.394979395081002	31.663888731483418	13.94553423308472
6	20.9510503721391	40.73943228514316	28.124063301750198	10.185454040967542
7	86.83891818801769	3.7696157070252125	7.919049731227444	1.4724163737296565
8	87.91408657387777	2.756350008109096	7.749364461839376	1.5801989561737575
9	83.58922252335518	5.064804375394464	8.997539915313684	2.3484331859366785
10	43.09759638588353	28.537607640917518	16.88360614415432	11.48118982904463
11	35.6695084324825	23.576912329928813	25.6011380089923	15.152441228596384
12	32.881659961193584	22.25471939597984	28.799834300888485	16.063786341938094
13	22.264723870637454	32.542680222208766	28.61948019719757	16.57311570995621
14	16.832684931346247	34.95551721375381	30.917617449992278	17.294180404907664
15	16.142884219699827	26.61838986577981	42.036809432343766	15.201916482176598
16	19.489498232607943	24.121296439227702	40.05428209101336	16.334923237150992
17	19.755242090700722	24.316891734779812	29.63942857254514	26.28843760197432
18	21.958571314122917	26.174675782723135	33.927010322976486	17.939742580177462
19	25.742373053572788	27.845735690377143	28.36870397111208	18.04318728493799
20	26.71640245344109	27.1206848375543	28.609592962477358	17.553319746527254
21	22.780149715400054	27.586283708924498	31.742205009662527	17.89136156601292
22	22.49353714845116	24.521436345552992	30.795922394977442	22.189104111018406
23	19.22508309380563	28.5992758479867	30.117454877279094	22.05818618092858
24	20.156319916525163	26.169908025269113	36.01255248929697	17.661219568908752
25	18.690781619122614	27.598203102559545	33.62277268543939	20.088242592878455
26	18.128928759152043	32.697671419443466	29.509917521704043	19.66348229970045
27	18.422849282198484	32.07973878941949	31.779526389732908	17.71788553864912
28	16.69742912357285	29.34918156753704	36.30100181526503	17.65238749362508
29	17.83434387646037	26.332089938663973	35.68900934206901	20.144556842806647
30	19.052974865711423	29.800125446733013	32.53181598801026	18.615083699545306
31	24.80105359623738	27.531884377973743	28.81913981057934	18.847922215209536
32	25.209869257929817	27.60832481715455	30.329346524128958	16.852459400786678
33	24.2635475632656	28.55273159284133	28.42662050018465	18.757100343708416
34	19.45764804961594	29.362077960650367	30.166695650984522	21.013578338749166
35	19.897493214738624	28.47554863405703	31.037358506050555	20.58959964515379
36	25.568779786271595	27.110680362896687	29.533834468932397	17.786705381899324
37	20.79633273475832	30.617756770117882	30.523300460557557	18.062610034566244
38	20.289699885300262	31.58791725205219	27.10474020606873	21.017642656578822
39	20.32448106672711	30.508059268696353	29.576939685914205	19.590519978662332
40	22.53675960537037	28.342833024927167	27.652641513489435	21.467765856213035
41	19.131760103640104	27.26363940121656	29.18688241420479	24.417718080938545
42	21.62017777482507	27.103685046632187	28.098622235335725	23.17751494320702
43	21.204484036805525	27.078908339862945	29.114662612770164	22.601945010561366
44	19.289760459267917	30.442131343901863	29.20442932483474	21.063678871995485
45	19.09170312503053	33.3591391462197	26.67001451821225	20.879143210537528
46	21.81706270968851	31.06881788925125	27.489013640866716	19.625105760193524
47	20.725402572635026	29.14502775655518	29.001799633038992	21.127770037770798
48	21.64081200380639	26.991525506525377	30.5504610460538	20.81720144361443
49	20.606755755992427	26.368199839381283	31.14713416743036	21.877910237195934
50	19.674151134003296	30.68352837499585	29.3761076731585	20.266212817842355
51	19.16431372625647	30.947318234132066	27.74154846601312	22.146819573598346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1433.0
1	2318.0
2	3203.0
3	17055.0
4	30907.0
5	22523.5
6	14140.0
7	14224.0
8	14308.0
9	15399.5
10	16491.0
11	17071.5
12	17652.0
13	17259.5
14	16867.0
15	16113.5
16	15360.0
17	14429.0
18	13498.0
19	13057.0
20	12616.0
21	13331.5
22	14047.0
23	14971.0
24	15895.0
25	17319.5
26	23567.5
27	28391.0
28	33695.0
29	38999.0
30	45663.0
31	52327.0
32	64235.0
33	76143.0
34	81439.5
35	86736.0
36	95027.5
37	103319.0
38	118038.5
39	132758.0
40	155579.0
41	178400.0
42	204074.0
43	229748.0
44	230725.5
45	231703.0
46	221535.0
47	211367.0
48	205176.5
49	198986.0
50	194262.5
51	189539.0
52	173425.0
53	157311.0
54	139107.5
55	120904.0
56	106525.0
57	92146.0
58	82204.5
59	72263.0
60	61860.0
61	51457.0
62	44940.0
63	38423.0
64	31584.5
65	24746.0
66	19993.5
67	15241.0
68	12573.5
69	9906.0
70	7839.0
71	5772.0
72	4616.5
73	3461.0
74	2512.0
75	1305.5
76	1048.0
77	771.0
78	494.0
79	401.5
80	309.0
81	217.5
82	126.0
83	91.0
84	56.0
85	37.5
86	19.0
87	17.5
88	16.0
89	12.5
90	9.0
91	4.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	3.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2558855.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.132922754156045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.14127196039588	18.770347764338055
2	7.6605152059936374	3.5442021303457714
3	2.5222835247849287	1.7504336982879058
4	1.2503863416546221	1.1570036261739258
5	0.7662171701129145	0.8862421304565046
6	0.5193041484902559	0.720781365176272
7	0.39269179954617245	0.6358876345564496
8	0.30560644268763143	0.5655656185492315
9	0.2656192340370815	0.5530094300698112
>10	3.0095453737481592	16.49510682537361
>50	1.1832362709648847	20.006064934898177
>100	0.9805977050477347	33.67131763932804
>500	0.0023842197192451156	0.3328542125361954
>1k	1.7030140851750827E-4	0.13737278247292145
>5k	0.0	0.0
>10k+	1.7030140851750827E-4	0.7738102074370872
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19642	0.7676089500968206	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3487	0.1362718872308122	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9079979131291145E-5	0.0	0.0	0.03556278100947494	0.0
2	3.9079979131291145E-5	0.0	0.0	0.11485605866686467	0.0
3	3.9079979131291145E-5	0.0	0.0	0.18027594373264605	0.0
4	3.9079979131291145E-5	0.0	0.0	0.2634381393240336	0.0
5	3.9079979131291145E-5	0.0	0.0	0.47278958753036027	0.0
6	3.9079979131291145E-5	0.0	0.0	0.727786451362035	0.0
7	3.9079979131291145E-5	0.0	0.0	0.8983314802909895	0.0
8	3.9079979131291145E-5	0.0	0.0	1.2797911565915223	0.0
9	3.9079979131291145E-5	0.0	0.0	1.4184860025284747	0.0
10	3.9079979131291145E-5	0.0	0.0	1.6674645495739304	0.0
11	3.9079979131291145E-5	0.0	0.0	1.9729918264223647	0.0
12	3.9079979131291145E-5	0.0	0.0	2.2088785804588382	0.0
13	3.9079979131291145E-5	0.0	0.0	2.313183044760254	0.0
14	3.9079979131291145E-5	0.0	0.0	2.3610169392169547	0.0
15	3.9079979131291145E-5	0.0	0.0	2.4292115028010577	0.0
16	3.9079979131291145E-5	0.0	0.0	2.5926048955489858	0.0
17	3.9079979131291145E-5	0.0	0.0	2.7871841116436844	0.0
18	3.9079979131291145E-5	0.0	0.0	3.031433981214254	0.0
19	3.9079979131291145E-5	0.0	0.0	3.1692690676103177	0.0
20	3.9079979131291145E-5	0.0	0.0	3.3151546297074277	0.0
21	3.9079979131291145E-5	0.0	0.0	3.520285440167575	0.0
22	3.9079979131291145E-5	0.0	0.0	3.7391333233028052	0.0
23	3.9079979131291145E-5	0.0	0.0	3.9878383104943422	0.0
24	3.9079979131291145E-5	0.0	0.0	4.168270574143514	0.0
25	3.9079979131291145E-5	0.0	0.0	4.334712205263682	0.0
26	3.9079979131291145E-5	0.0	0.0	4.501466476216902	0.0
27	3.9079979131291145E-5	0.0	0.0	4.657161113075966	0.0
28	3.9079979131291145E-5	0.0	0.0	4.824814223549205	0.0
29	3.9079979131291145E-5	0.0	0.0	5.009193565090636	0.0
30	3.9079979131291145E-5	0.0	0.0	5.22632192914409	0.0
31	3.9079979131291145E-5	0.0	0.0	5.440714694658353	0.0
32	3.9079979131291145E-5	0.0	0.0	5.635098510857395	0.0
33	3.9079979131291145E-5	0.0	0.0	5.835188004009606	0.0
34	3.9079979131291145E-5	0.0	0.0	6.043132572967206	0.0
35	3.9079979131291145E-5	0.0	0.0	6.279410126794993	0.0
36	3.9079979131291145E-5	0.0	0.0	6.5031430073216345	0.0
37	3.9079979131291145E-5	0.0	0.0	6.742820519333843	0.0
38	3.9079979131291145E-5	0.0	0.0	6.9874221087165935	0.0
39	3.9079979131291145E-5	0.0	0.0	7.272002516750656	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGACCG	30	2.1665492E-6	45.000004	1
TAACGAC	25	3.8921513E-5	45.0	28
TACGACG	80	0.0	45.0	1
GTACGTA	20	7.034911E-4	45.0	31
TAATCCG	20	7.034911E-4	45.0	1
CGTTTTT	12805	0.0	43.31316	1
CGACGGT	355	0.0	43.09859	28
TTAGACG	155	0.0	40.64516	1
CACGACG	380	0.0	40.263157	26
TCCGATT	40	3.4604454E-7	39.375	29
TCGTGCG	40	3.4604454E-7	39.375	1
CGGTCTA	400	0.0	39.375	31
GTTGCGA	40	3.4604454E-7	39.375	37
TCACGAC	395	0.0	39.3038	25
TCGATAG	75	0.0	39.0	1
AGTACGG	295	0.0	38.898304	2
GTACGGG	1010	0.0	38.76238	3
CGGCGAA	35	6.2512954E-6	38.571426	11
TAGTACG	140	0.0	38.571426	1
TAACGGG	505	0.0	38.316833	3
>>END_MODULE