FastQCFastQC Report
Thu 26 May 2016
SRR1548141_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548141_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3800492
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400771.054521361971029No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC46390.12206314340353827No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG253.89251E-545.00000411
TCGGCGA453.8562575E-1045.031
CGACGGT5850.043.84615728
ACGCGAG1550.043.548391
CGTTTTT195150.043.305151
TACGGGA17450.042.5501444
TCACGAC6250.042.1225
CCGAACG650.041.5384641
CGGTCTA6200.041.3709731
CGCGAGG5450.039.633032
GTGCGAC403.4610275E-739.37522
CACGCCG2750.039.27272826
CACGACG6650.039.2481226
CGGGACT13300.039.0789456
TCGATAG2600.038.942311
TAGGGAC44600.038.8957375
GCCGATC1100.038.8636369
CTCACGA6900.038.80434824
GACGGTC6900.038.80434829
ACGCCGG2800.038.57142627
Produced by FastQC (version 0.11.5)