##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548141_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3800492
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.112970899557215	31.0	31.0	33.0	30.0	34.0
2	31.48808364811714	31.0	31.0	34.0	30.0	34.0
3	31.53545277821924	31.0	31.0	34.0	30.0	34.0
4	35.36607234010754	37.0	35.0	37.0	33.0	37.0
5	35.22476326749273	37.0	35.0	37.0	33.0	37.0
6	35.27608267561148	37.0	35.0	37.0	32.0	37.0
7	35.7810688721355	37.0	35.0	37.0	35.0	37.0
8	35.7976940880286	37.0	35.0	37.0	35.0	37.0
9	37.533527238052336	39.0	37.0	39.0	35.0	39.0
10	36.78848922718427	39.0	37.0	39.0	32.0	39.0
11	36.39198056462163	38.0	35.0	39.0	32.0	39.0
12	35.90610426229025	37.0	35.0	39.0	31.0	39.0
13	35.69204908206622	37.0	35.0	39.0	30.0	39.0
14	36.64790506071319	38.0	35.0	41.0	31.0	41.0
15	36.9226979033241	39.0	35.0	41.0	31.0	41.0
16	37.026430525310936	39.0	35.0	41.0	32.0	41.0
17	36.89431289422527	38.0	35.0	41.0	31.0	41.0
18	36.79530860741188	38.0	35.0	40.0	31.0	41.0
19	36.694888451284726	38.0	35.0	40.0	31.0	41.0
20	36.548744741470315	38.0	35.0	40.0	31.0	41.0
21	36.40043999566372	38.0	35.0	40.0	31.0	41.0
22	36.321891481418724	38.0	35.0	40.0	30.0	41.0
23	36.283883244590434	38.0	35.0	40.0	30.0	41.0
24	36.117512153689574	38.0	34.0	40.0	30.0	41.0
25	36.0135211441045	38.0	34.0	40.0	30.0	41.0
26	35.934549526745485	38.0	34.0	40.0	30.0	41.0
27	35.906799698565344	38.0	34.0	40.0	30.0	41.0
28	35.92183485717113	38.0	34.0	40.0	30.0	41.0
29	35.95643300919986	38.0	35.0	40.0	30.0	41.0
30	35.858950367478734	38.0	34.0	40.0	30.0	41.0
31	35.709413412789715	38.0	34.0	40.0	29.0	41.0
32	35.52723673671725	38.0	34.0	40.0	29.0	41.0
33	35.371244038929696	38.0	34.0	40.0	27.0	41.0
34	35.20004067894367	38.0	34.0	40.0	27.0	41.0
35	35.01850181502816	38.0	34.0	40.0	25.0	41.0
36	34.852706702184875	38.0	34.0	40.0	24.0	41.0
37	34.76297963526827	37.0	34.0	40.0	24.0	41.0
38	34.68858479375828	37.0	33.0	40.0	24.0	41.0
39	34.67177670680533	37.0	33.0	40.0	24.0	41.0
40	34.5651826131985	37.0	33.0	40.0	24.0	41.0
41	34.514773087273966	37.0	33.0	40.0	23.0	41.0
42	34.461787842205695	37.0	33.0	40.0	23.0	41.0
43	34.371664247681615	37.0	33.0	40.0	23.0	41.0
44	34.24261358792493	37.0	33.0	40.0	23.0	41.0
45	34.169103368721736	37.0	33.0	40.0	23.0	41.0
46	34.0521629831085	37.0	33.0	40.0	23.0	41.0
47	34.02283599070857	36.0	33.0	40.0	23.0	41.0
48	33.924304274288694	36.0	33.0	40.0	23.0	41.0
49	33.8206942680053	36.0	33.0	40.0	23.0	41.0
50	33.67112679095233	36.0	32.0	40.0	22.0	41.0
51	33.33008515739541	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	2.0
11	4.0
12	8.0
13	8.0
14	38.0
15	109.0
16	330.0
17	844.0
18	2043.0
19	4060.0
20	6923.0
21	11176.0
22	17145.0
23	24818.0
24	36455.0
25	51600.0
26	68715.0
27	79443.0
28	86984.0
29	96512.0
30	114448.0
31	136686.0
32	167671.0
33	211118.0
34	317812.0
35	413427.0
36	301168.0
37	364182.0
38	516705.0
39	769943.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.034850224655123	24.062174055359147	30.36959425253362	14.533381467452108
2	31.178279022821254	25.149533270955445	30.69905159647751	12.973136109745791
3	27.805741993405064	25.090014661259648	34.2430401116487	12.861203233686586
4	25.15040157958496	27.22155447242094	33.60259671642514	14.025447231568966
5	22.805389407476717	31.88442443767807	31.723839965983352	13.586346188861864
6	21.29145384334449	40.095229775513275	28.355960228307282	10.257356152834948
7	87.85615125620578	3.0577356826431945	7.813725170320053	1.2723878908309767
8	89.20466087022417	2.201504436793973	7.3311560713718125	1.2626786216100443
9	84.90798033517765	4.905917444372991	8.50650389475889	1.6795983256904634
10	43.22913980610931	35.95950208551945	13.063677018659689	7.747681089711543
11	31.22985129293786	23.781789305174172	30.15417477526594	14.834184626622026
12	30.178566354040477	23.04683183124711	30.85595233459247	15.918649480119942
13	22.332871638724672	31.15420319263927	30.394959389468525	16.11796577916754
14	16.137410629992115	34.96168390829398	31.861637914249	17.039267547464906
15	15.151722461197128	28.097177944329317	41.65358064166429	15.097518952809269
16	19.29055501235103	26.07783413305435	39.12448704009902	15.507123814495596
17	19.502185506508106	25.068596381731627	30.86952952407215	24.559688587688118
18	20.82298818153018	26.731959967288443	34.86545952471417	17.579592326467207
19	23.709377627949223	27.697203414715776	30.40214266994905	18.19127628738595
20	25.68801618316786	28.22150395264613	29.8260856752231	16.264394188962903
21	22.415834581417354	27.109226910621047	33.024829416822875	17.450109091138728
22	21.628199717299758	24.36642413666441	32.25884964367771	21.746526502358115
23	18.104340174903673	28.813216815086047	32.199462595895476	20.882980414114805
24	18.69029062552954	26.47841384747027	37.595869166413195	17.235426360587
25	18.210089641025426	28.436160370815145	34.7556842640374	18.598065724122034
26	17.71273298299273	33.787257018301844	30.081578911362	18.41843108734343
27	17.146990442290104	32.649141216453025	33.17859898139504	17.025269359861827
28	15.597138475755246	30.13196712425654	37.309011570080926	16.961882829907285
29	16.867894998858045	27.36585157921659	36.250438101172165	19.51581532075321
30	18.5206546941817	29.998510719138473	33.57007461139242	17.91075997528741
31	23.44583280270028	28.59379785564606	29.722204388273937	18.23816495337972
32	23.323006600198077	28.457789149404867	31.007011723745244	17.212192526651812
33	22.724768266845448	29.587274489723963	29.475683674640017	18.212273568790568
34	18.62982477005609	29.961515509044617	30.869108525948747	20.539551194950548
35	18.18448769264611	29.54164881810039	31.628247079588647	20.645616409664854
36	24.12324509563499	27.92564752142617	30.527179112599107	17.423928270339736
37	19.156151361455308	31.12796974707485	32.10008072639016	17.61579816507968
38	18.51283991651607	31.31768202643237	29.48034096638014	20.68913709067142
39	18.873582683505187	30.7319420748682	31.063030786540267	19.33144445508634
40	20.999912642889395	28.807033405148598	29.670895241984457	20.52215870997755
41	18.32220670376362	27.543670661588028	30.627402978351228	23.506719656297133
42	20.826935038937062	27.904123992367303	29.619901844287526	21.649039124408105
43	21.084875326668232	28.095336077539436	29.461290801296254	21.35849779449608
44	18.948546661853253	30.886711509983446	30.359963920460824	19.804777907702476
45	18.762465491310074	33.675666203217894	27.563378636239726	19.9984896692323
46	21.034829174748953	31.39001476650918	28.65636870173651	18.918787357005357
47	19.421432803963278	30.546571338658257	29.20040352670128	20.831592330677186
48	20.788308461115037	28.77430080105418	31.14452023580105	19.29287050202974
49	21.008779915863528	27.270679690945276	31.19901318039875	20.52152721279245
50	18.398091615506623	31.608302293492528	30.21329869922105	19.7803073917798
51	18.446269588253312	32.12023601154798	28.50167820376941	20.931816196429303
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1895.0
1	3317.5
2	4740.0
3	31251.0
4	57762.0
5	39877.5
6	21993.0
7	22088.5
8	22184.0
9	24217.5
10	26251.0
11	27407.0
12	28563.0
13	28541.0
14	28519.0
15	26957.5
16	25396.0
17	24142.0
18	22888.0
19	22428.0
20	21968.0
21	21753.5
22	21539.0
23	25232.5
24	28926.0
25	32492.0
26	44164.0
27	52270.0
28	63563.0
29	74856.0
30	86934.5
31	99013.0
32	114297.0
33	129581.0
34	142944.5
35	156308.0
36	168364.5
37	180421.0
38	193707.0
39	206993.0
40	240057.0
41	273121.0
42	302803.5
43	332486.0
44	345864.5
45	359243.0
46	347214.0
47	335185.0
48	319250.0
49	303315.0
50	277677.0
51	252039.0
52	225999.0
53	199959.0
54	179858.5
55	159758.0
56	136005.0
57	112252.0
58	96593.5
59	80935.0
60	70252.5
61	59570.0
62	48641.0
63	37712.0
64	29658.0
65	21604.0
66	16505.5
67	11407.0
68	8681.0
69	5955.0
70	4587.0
71	3219.0
72	2551.5
73	1884.0
74	1526.0
75	989.0
76	810.0
77	582.5
78	355.0
79	254.0
80	153.0
81	132.0
82	111.0
83	90.0
84	69.0
85	50.0
86	31.0
87	24.0
88	17.0
89	12.0
90	7.0
91	4.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3800492.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.57818034851778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09505185670143	16.48210422131086
2	8.340464744977814	3.4326317542521556
3	2.8764913986929463	1.7757887631979083
4	1.44006983706081	1.1853606728599186
5	0.8839331467966706	0.9094867855407365
6	0.6073845372203297	0.7499321128692574
7	0.4279184928025862	0.6164048743550268
8	0.32773918413487957	0.5395420830722902
9	0.23640412898188198	0.4378290121191085
>10	2.473558267846509	11.953576633201665
>50	0.9813922787429824	14.821387294867527
>100	1.3036569307968733	45.07463951834971
>500	0.004773961392088546	0.6320513063702926
>1k	0.0010322078685596856	0.32517254144578767
>5k	0.0	0.0
>10k+	1.290259835699607E-4	1.0640924261878688
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40077	1.054521361971029	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4639	0.12206314340353827	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.262476542510812E-5	0.0	0.0	0.03502178139040945	0.0
2	5.262476542510812E-5	0.0	0.0	0.12624681225483436	0.0
3	5.262476542510812E-5	0.0	0.0	0.1945537577766247	0.0
4	5.262476542510812E-5	0.0	0.0	0.28217399220942974	0.0
5	5.262476542510812E-5	0.0	0.0	0.5223007968441981	0.0
6	5.262476542510812E-5	0.0	0.0	0.7843984410439491	0.0
7	5.262476542510812E-5	0.0	0.0	0.9740054708706136	0.0
8	5.262476542510812E-5	0.0	0.0	1.3471413701173427	0.0
9	5.262476542510812E-5	0.0	0.0	1.48217651819817	0.0
10	5.262476542510812E-5	0.0	0.0	1.7213034522898614	0.0
11	5.262476542510812E-5	0.0	0.0	2.0620224960347238	0.0
12	5.262476542510812E-5	0.0	0.0	2.318278791272288	0.0
13	7.893714813766218E-5	0.0	0.0	2.4270278690232736	0.0
14	7.893714813766218E-5	0.0	0.0	2.4755479027452236	0.0
15	7.893714813766218E-5	0.0	0.0	2.544907343575516	0.0
16	7.893714813766218E-5	0.0	0.0	2.7018343940731886	2.631238271255406E-5
17	7.893714813766218E-5	0.0	0.0	2.911438834761394	2.631238271255406E-5
18	7.893714813766218E-5	0.0	0.0	3.152433948025677	2.631238271255406E-5
19	7.893714813766218E-5	0.0	0.0	3.296967866265736	2.631238271255406E-5
20	7.893714813766218E-5	0.0	0.0	3.4481588173320716	2.631238271255406E-5
21	7.893714813766218E-5	0.0	0.0	3.6399760873065907	2.631238271255406E-5
22	7.893714813766218E-5	0.0	0.0	3.868683317844111	2.631238271255406E-5
23	7.893714813766218E-5	0.0	0.0	4.102810899220417	2.631238271255406E-5
24	7.893714813766218E-5	0.0	0.0	4.278314491913152	2.631238271255406E-5
25	7.893714813766218E-5	0.0	0.0	4.430873686880541	2.631238271255406E-5
26	7.893714813766218E-5	0.0	0.0	4.583169758020803	2.631238271255406E-5
27	7.893714813766218E-5	0.0	0.0	4.738018130284185	2.631238271255406E-5
28	7.893714813766218E-5	0.0	0.0	4.907496187335745	2.631238271255406E-5
29	7.893714813766218E-5	0.0	0.0	5.092024927298887	2.631238271255406E-5
30	7.893714813766218E-5	0.0	0.0	5.317627296676325	2.631238271255406E-5
31	7.893714813766218E-5	0.0	0.0	5.514128170773679	2.631238271255406E-5
32	7.893714813766218E-5	0.0	0.0	5.696499295354391	2.631238271255406E-5
33	7.893714813766218E-5	0.0	0.0	5.887606130995671	2.631238271255406E-5
34	7.893714813766218E-5	0.0	0.0	6.088606422536872	2.631238271255406E-5
35	7.893714813766218E-5	0.0	0.0	6.324865306912894	2.631238271255406E-5
36	7.893714813766218E-5	0.0	0.0	6.5293651453548645	2.631238271255406E-5
37	7.893714813766218E-5	0.0	0.0	6.7455476817212086	2.631238271255406E-5
38	7.893714813766218E-5	0.0	0.0	6.972281483555287	2.631238271255406E-5
39	7.893714813766218E-5	0.0	0.0	7.249903433555445	2.631238271255406E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGCG	25	3.89251E-5	45.000004	11
TCGGCGA	45	3.8562575E-10	45.0	31
CGACGGT	585	0.0	43.846157	28
ACGCGAG	155	0.0	43.54839	1
CGTTTTT	19515	0.0	43.30515	1
TACGGGA	1745	0.0	42.550144	4
TCACGAC	625	0.0	42.12	25
CCGAACG	65	0.0	41.538464	1
CGGTCTA	620	0.0	41.37097	31
CGCGAGG	545	0.0	39.63303	2
GTGCGAC	40	3.4610275E-7	39.375	22
CACGCCG	275	0.0	39.272728	26
CACGACG	665	0.0	39.24812	26
CGGGACT	1330	0.0	39.078945	6
TCGATAG	260	0.0	38.94231	1
TAGGGAC	4460	0.0	38.895737	5
GCCGATC	110	0.0	38.863636	9
CTCACGA	690	0.0	38.804348	24
GACGGTC	690	0.0	38.804348	29
ACGCCGG	280	0.0	38.571426	27
>>END_MODULE